<p><strong>🎤Dr. Christopher Reid (Professor of Chemistry and Biochemistry at Bryant University in Rhode Island)</strong></p><p><strong>In this episode of Concentrating on Chromatography, host Dave Oliva sits down with Chris Reid to explore how chemical biology and LC-MS are being used to tackle one of the most urgent global challenges: antimicrobial resistance.</strong></p><p>As antibiotic resistance continues to rise—and new drug development struggles to keep pace—researchers are looking beyond traditional approaches. Instead of targeting how bacteria build their cell walls, Chris’s work focuses on how they break them down, opening the door to entirely new antimicrobial strategies.</p><h4><strong>What You’ll Learn in This Episode:</strong></h4><ul><li>Why antimicrobial resistance is accelerating globally</li><li>What peptidoglycan is and why it’s essential to bacterial survival</li><li>How targeting cell wall degradation differs from traditional antibiotics</li><li>The strategy behind designing small molecule probes and inhibitors</li><li>How LC-MS is used to analyze peptidoglycan fragments and enzyme activity</li><li>Key analytical challenges: complex mixtures, low-abundance species, and structural similarity</li><li>The importance of sample preparation and reproducibility in LC-MS workflows</li><li>How this research could lead to new antibiotic targets</li></ul><h3><strong>Video Transcription</strong></h3><h4><strong>The Growing Challenge of Antibiotic Resistance</strong></h4><p>According to Reid, antibiotic resistance is increasing across a broad spectrum of bacterial pathogens that were once readily treated with existing therapies. In some cases, clinicians must now rely on only a handful of last-line antibiotics, while resistance is also rising in diseases such as tuberculosis.</p><p>One of the major obstacles is the limited commercial incentive to develop entirely new antibiotics. Because bacterial resistance can emerge relatively quickly after a drug reaches the market, pharmaceutical companies often favor modifying existing antibiotics or combining them with resistance-modifying agents rather than investing in completely new drug classes. As a result, genuinely novel antibacterial targets remain urgently needed.</p><h4><strong>Why the Bacterial Cell Wall Remains an Attractive Target</strong></h4><p>The bacterial cell wall consists primarily of <strong>peptidoglycan</strong>, a highly cross-linked polymer that provides structural integrity, maintains cell shape, and enables bacterial growth and division.</p><p>Many widely used antibiotics—including the β-lactam antibiotics such as penicillins—target enzymes responsible for synthesizing or cross-linking peptidoglycan. These pathways have proven highly successful therapeutic targets because peptidoglycan is unique to bacteria and absent from human cells, minimizing unwanted effects on the host.</p><p>However, most existing antibiotics target only specific stages of peptidoglycan biosynthesis. Reid's research explores another part of this complex pathway: enzymes responsible for degrading the carbohydrate backbone of peptidoglycan during normal bacterial growth and remodeling. These enzymes may represent an underexplored class of antimicrobial targets capable of overcoming existing resistance mechanisms.</p><h4><strong>Targeting the Glycan Backbone</strong></h4><p>A key advantage of focusing on the carbohydrate backbone is its remarkable conservation across both Gram-positive and Gram-negative bacteria.</p><p>Whereas the peptide components of peptidoglycan vary considerably between bacterial species—and frequently acquire mutations associated with antibiotic resistance—the glycan backbone remains highly conserved and tolerates relatively few structural modifications.</p><p>This biological constraint makes enzymes acting on the glycan backbone particularly attractive targets. Reid's group developed a series of <strong>small-molecule probes and inhibitors</strong> designed to recognize enzymes known as <strong>N-acetylglucosaminidases</strong>, which cleave glycosidic bonds within peptidoglycan and generate soluble degradation products.</p><p>Rather than beginning with purified enzymes, the researchers adopted a <strong>top-down phenotypic screening strategy</strong>. Candidate compounds were first evaluated for their ability to inhibit bacterial growth before progressively narrowing the list of potential molecular targets through genetic studies, microscopy, enzyme assays, and biochemical characterization. This iterative workflow ultimately identified compounds capable of inhibiting the desired enzyme class while simultaneously providing valuable chemical probes for studying bacterial cell wall biology.</p><h4><strong>LC-MS as a Central Analytical Tool</strong></h4><p>Liquid chromatography coupled with mass spectrometry plays a central role throughout the project by enabling detailed characterization of structural changes occurring within peptidoglycan after bacterial cells are treated with candidate inhibitors.</p><p>Because intact peptidoglycan is an insoluble polymer, sample preparation begins with isolation of the bacterial cell wall through detergent extraction and differential centrifugation. Additional purification steps remove proteins, nucleic acids, and teichoic acids before enzymatic digestion converts the insoluble material into soluble fragments suitable for chromatographic analysis.</p><p>The resulting digest is analyzed by LC-MS to examine changes in the distribution of peptidoglycan fragments. Chromatographic retention times provide an immediate indication of changes in cross-linking, with larger oligomers such as trimers and tetramers generally eluting later than monomeric fragments. Mass spectrometry then confirms the identity of these fragments by measuring their intact molecular masses, while tandem MS experiments can provide further structural information when required.</p><h4><strong>Sample Preparation Determines Data Quality</strong></h4><p>Reid emphasized that sample preparation represents one of the most critical—and challenging—stages of the entire workflow.</p><p>Because peptidoglycan analysis involves multiple purification, digestion, and reduction steps, even minor procedural differences between samples can introduce analytical artifacts that complicate biological interpretation.</p><p>Maintaining strict consistency throughout sample preparation is therefore essential. Appropriate controls must accompany every experiment, ensuring that observed chromatographic and mass spectrometric differences truly reflect biological responses rather than experimental variability. The complexity of bacterial cell wall composition further increases the analytical challenge, as subtle differences in amino acid substitutions and low-abundance cross-linked species can complicate LC-MS data interpretation.</p><h4><strong>Chemical Biology for Future Antibiotic Discovery</strong></h4><p>Although the identified compounds may eventually serve as starting points for antibiotic development, Reid views them primarily as <strong>chemical biology tools</strong>.</p><p>By selectively inhibiting individual enzymes, these probes allow researchers to investigate previously unexplored aspects of bacterial cell wall remodeling that cannot easily be studied using classical genetic approaches alone.</p><p>The research team is now expanding this strategy by combining their probes with <strong>proximity-labeling proteomics and mass spectrometry</strong>, aiming to map protein interaction networks associated with bacterial cell wall degradation. Such studies may reveal entirely new antibacterial targets and provide deeper insight into the biological processes governing bacterial growth and division.</p><p>Ultimately, the work illustrates how integrating synthetic chemistry, microbiology, molecular biology, and advanced LC-MS can accelerate the search for innovative therapeutic strategies against antimicrobial resistance. As existing antibiotics become progressively less effective, multidisciplinary approaches such as these may play an increasingly important role in identifying the next generation of antibacterial targets.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

 Fighting Antibiotic Resistance with LC-MS: Targeting Bacterial Cell Walls

<p><strong>This study compares a commercial ELISA assay with a validated UHPLC-MS/MS method for determining total ergot alkaloids in wheat and durum samples. In addition to method performance, the authors investigated cross-reactivity and matrix effects to explain differences between immunoassay and chromatographic results.</strong></p><p>While ELISA demonstrated good repeatability and is well suited for high-throughput screening, systematic biases were observed due to compound- and matrix-dependent cross-reactivity. The findings support ELISA as an effective screening tool, whereas UHPLC-MS/MS remains the preferred method for accurate quantification and regulatory confirmation of ergot alkaloids.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jafc.6c05234"><strong>Comparative Evaluation of Total Ergot Alkaloids Determination by ELISA and UHPLC-MS/MS in Wheat: Implications of Cross-Reactivity and Matrix Effects</strong></a></h4><p>Chamali Kodikara*, Dainna Drul, Nandika Bandara, and Sheryl A. Tittlemier*</p><p>J. Agric. Food Chem. 2026, 74, 25, 20015–20027</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1021/acs.jafc.6c05234">https://doi.org/10.1021/acs.jafc.6c05234</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p><strong>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.</strong></p><div><p>Ergot alkaloids (EAs) are a structurally diverse group of indole-derived mycotoxins produced primarily by phytopathogenic fungi of the genus <i>Claviceps</i>, most notably <i>Claviceps purpurea</i>. These fungi infect the flowering heads of cereal crops, replacing the developing grain with hardened fungal structures (sclerotia) that contain ergot alkaloids. Cereals such as rye, wheat, barley, oats, and triticale are particularly susceptible, with infection severity strongly influenced by environmental conditions during flowering, including rainfall and humidity. <a href="javascript:void(0);">(1−3)</a> Although improvements in agronomic practices and grain cleaning have reduced the prevalence of visible ergot sclerotia, ergot alkaloids can persist in cereals and cereal-based foods even in the absence of detectable sclerotia. This may occur because sclerotia can fragment during harvesting, handling, cleaning, or milling, producing small particles that are not visually detected but may still contribute ergot alkaloids to grain or processed products, continuing to pose food and feed safety concerns. <a href="javascript:void(0);">(4)</a> <a href="javascript:void(0);">(5)</a> Ergot alkaloids share a common tetracyclic ergoline backbone and occur as structurally related compounds, including ergometrine, ergotamine, ergosine, ergocristine, ergocryptine, and ergocornine, together with their corresponding C-8 epimers. These epimers occur as <i>R-</i> and <i>S-</i>configurations, commonly referred to as -ine and -inine forms, respectively. Epimerization at the C-8 position can occur during fungal metabolism and during sample extraction, storage, and analysis, complicating accurate quantification. <a href="javascript:void(0);">(6,7)</a> Although S-epimers were historically considered biologically inactive, recent evidence indicates that they may contribute to overall exposure and toxicity, supporting their inclusion in analytical and regulatory frameworks. <a href="javascript:void(0);">(3)</a></p><p>Dietary exposure to elevated levels of ergot alkaloids has been associated with a range of adverse health effects, collectively referred to as ergotism, including vasoconstriction, ischemia, neurological disturbances, and hallucinations. <a href="javascript:void(0);">(3)</a> These effects arise primarily from interactions with adrenergic, serotonergic, and dopaminergic receptors. <a href="javascript:void(0);">(8−10)</a> In response, regulatory limits for ergot alkaloids in food have been introduced or revised in several jurisdictions. In the European Union, maximum levels have been established for the sum of 12 ergot alkaloids in cereal milling products under Regulation (EU) 2021/1399, which was subsequently consolidated under Regulation (EU) 2023/915. This sum includes ergometrine, ergosine, ergotamine, ergocornine, ergocryptine, and ergocristine together with their corresponding -inine epimers, increasing the demand for reliable analytical methods to support routine monitoring and enforcement. <a href="javascript:void(0);">(1,11)</a></p><p>Liquid chromatography-tandem mass spectrometry (LC-MS/MS), particularly ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), is widely regarded as the reference technique for confirmatory determination of ergot alkaloids due to its sensitivity, selectivity, and ability to resolve epimeric forms. Recent methods routinely achieve low microgram-per-kilogram limits of quantification across a range of cereal matrices. <a href="javascript:void(0);">(12−17)</a> However, LC-MS/MS approaches are resource-intensive and typically confined to centralized laboratories, limiting their suitability for high-throughput screening or rapid decision-making in grain-handling and quality-control settings. Consequently, rapid screening methods, most notably enzyme-linked immunosorbent assays (ELISAs), are commonly employed as complementary tools to chromatographic analysis. <a href="javascript:void(0);">(18−21)</a> ELISAs offer reduced analysis time, simplified workflows, and lower per-sample costs, making them attractive for preliminary screening and sample triage. <a href="javascript:void(0);">(22−24)</a></p><p>In contrast to other regulated mycotoxins, however, the commercial availability of ELISAs for ergot alkaloids is limited. While multiple kits have been evaluated, performance has varied substantially across assays and matrices. <a href="javascript:void(0);">(1)</a> At present, the Randox Food Diagnostics Ergot Alkaloids ELISA is the only widely marketed microplate ELISA that quantifies the sum of 12 major ergot alkaloids, comprising ergometrine, ergosine, ergotamine, ergocornine, ergocryptine, and ergocristine together with their corresponding -inine epimers, consistent with the analyte scope used for EU maximum levels for ergot alkaloids in specified cereal products. <a href="javascript:void(0);">(25)</a></p><p>In addition, cereal matrix effects may influence antibody binding and signal generation, potentially resulting in biased quantification relative to LC-MS/MS reference methods. These limitations are particularly relevant given the compositional variability of ergot alkaloids across cereal species, geographic regions, and growing conditions. <a href="javascript:void(0);">(12)</a> Recent reviews emphasize the need for systematic, matrix-specific evaluation of immunoassay performance before such methods can be confidently integrated into regulatory or industrial monitoring programs. <a href="javascript:void(0);">(1,6,11)</a> However, independent evaluations of commercially available ergot alkaloid ELISAs, particularly those benchmarked against validated UHPLC-MS/MS methods using naturally infested wheat samples, remain limited.</p><p>In this context, we hypothesized that measurements of total ergot alkaloids obtained using a commercially available ELISA are influenced by matrix effects and antibody cross-reactivity, resulting in systematic differences compared to UHPLC-MS/MS, which provides compound-specific quantification. To test this, the objective of the present study was to conduct a comprehensive performance evaluation of a commercially available ELISA for the detection of ergot alkaloids in wheat, using UHPLC-MS/MS as a reference method. Specific aims included assessment of repeatability and intermediate precision, extraction efficiency and extract stability, the influence of test portion mass, method accuracy relative to UHPLC-MS/MS, and characterization of antibody cross-reactivity toward individual ergot alkaloids in both solvent and wheat matrix. The results provide data-driven guidance on the appropriate use of ELISA-based screening within tiered analytical strategies for regulatory monitoring and food safety decision-making.</p><h4><strong>2. Materials and Methods</strong></h4><h5><strong>2.3. UHPLC-MS/MS Reference Method</strong></h5><p>UHPLC-MS/MS analysis of ergot alkaloids was performed using the validated Canadian Grain Commission (CGC) method described by Tittlemier et al. (2015). Briefly, ground grain (10 g) was extracted with 50 mL of acetonitrile/aqueous ammonium carbonate (84:16, v/v; 3.03 mM ammonium carbonate) to provide alkaline extraction conditions, then shaken for 30 min and centrifuged at 2450<i>g</i> for 10 min at room temperature. An aliquot of extract (464 μL) was combined with internal standard solution (dihydroergotamine; 20 μL of 4 ng/μL) and aqueous ammonium carbonate (1536 μL), vortex-mixed, and filtered through a 0.45 μm polytetrafluoroethylene syringe filter before injection into the UHPLC-MS/MS system. As in the validated reference method, dihydroergotamine was added after extraction to correct for postextraction analytical variability; extraction recovery was evaluated during method validation. Chromatographic separation was achieved using a reversed-phase <strong>BEH C18 column</strong> (2.1 × 100 mm, 1.7 μm; Waters, Milford, MA, USA) with the autosampler maintained at 15 °C and the column at 30 °C. Mobile phases consisted of (A) 3.03 mM aqueous ammonium carbonate and (B) acetonitrile. The gradient program was as follows: initial, 75% A/25% B; 1.00 min, 60% A/40% B; 5.00 min, 40% A/60% B; 8.00 min, 22% A/78% B; 10.50–11.00 min, 10% A/90% B; and 13.00–16.00 min, 75% A/25% B, with a total run time of 16 min. Detection was performed using positive electrospray ionization in multiple reaction monitoring mode on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/554"><strong>Waters Xevo triple quadrupole mass spectrometer</strong></a> (Waters, Milford, MA, USA). Under the validated chromatographic conditions, dihydroergotamine did not coelute with ergotamine, ergotaminine, or the other target ergot alkaloids. Identification and quantification were based on retention-time criteria and compound-specific MRM transitions, thereby minimizing potential isotopic or coelution interference. Precautions to minimize epimerization, including controlled handling of standards and extracts and reduced light exposure, were followed as described in the reference method. UHPLC-MS/MS quantification was performed using analyte-specific, matrix-matched calibration curves prepared according to the validated CGC reference method, with calibration levels of 0.046, 0.2, 1, 2, 10, and 20 μg/kg. Dihydroergotamine was used as the internal standard, and concentrations were calculated from internal-standard-normalized analyte responses with method dilution factors applied. Total ergot alkaloid concentration was calculated as the arithmetic sum of all quantified ergot alkaloids and epimers included in the validated method. <a href="javascript:void(0);">(26)</a></p><h4><strong>3. Results and Discussion</strong></h4><h5><strong>3.4. Comparison of ELISA and UHPLC-MS/MS Results</strong></h5><p>For method comparison, UHPLC-MS/MS was used as the chemical reference method, providing compound-specific quantification of individual ergot alkaloids and their epimers. A total ergot alkaloid concentration was calculated as the arithmetic sum of all quantified alkaloids included in the method. This summed value represents a chemically defined total based on compound-specific quantification of each analyte, rather than an immunoreactivity-weighted response governed by antibody affinity. As such, the UHPLC-MS/MS total serves as a chemically defined comparator against which the immunoreactivity-weighted ELISA response can be interpreted. Agreement between the commercial ELISA and the validated UHPLC-MS/MS reference method was evaluated using wheat and durum wheat cargo samples (<i>n</i> = 102) collected over multiple crop years (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jafc.6c05234#fig5">Figure 5</a>). Linear regression analysis demonstrated a positive association between ELISA and UHPLC-MS/MS results across the full concentration range, indicating that the ELISA was responsive to increasing ergot alkaloid levels. However, the regression slope deviated substantially from unity, with ELISA results systematically lower than UHPLC-MS/MS values, indicating an approximate 2-fold underestimation.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/J_Agric_Food_Chem_2026_74_25_20015_20027_Figure_5_Linear_regression_analysis_comparing_total_ergot_alkaloid_concentrations_determined_by_ELISA_and_UHPLC_MS_MS_5d7b5c5c61.jpeg" alt="J. Agric. Food Chem. 2026, 74, 25, 20015–20027: Figure 5. Linear regression analysis comparing total ergot alkaloid concentrations determined by ELISA and UHPLC-MS/MS for wheat and durum wheat cargo samples (n = 102). Each point represents the mean of duplicate measurements (extracted from the same test portion) ELISA determinations for an individual cargo sample, plotted against the corresponding UHPLC-MS/MS result. Statistical analysis was performed using linear regression." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_J_Agric_Food_Chem_2026_74_25_20015_20027_Figure_5_Linear_regression_analysis_comparing_total_ergot_alkaloid_concentrations_determined_by_ELISA_and_UHPLC_MS_MS_5d7b5c5c61.jpeg 197w," sizes="100vw" width="992" height="784"></p><p>Bland-Altman analysis confirmed this proportional bias, showing a consistent negative mean difference between ELISA and UHPLC-MS/MS results across the concentration range (<a target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acs.jafc.6c05234#fig6">Figure 6</a>). Importantly, the magnitude of disagreement did not increase randomly with concentration; rather, it reflected a systematic offset, indicating that the discrepancy was not driven by analytical imprecision or outliers but by fundamental differences in method response characteristics. This pattern indicates method-level bias rather than random analytical variability, which may arise from combined workflow effects including extraction, matrix response, and immunochemical cross-reactivity. The magnitude of the ELISA-UHPLC-MS/MS bias was substantially greater than the intermediate precision observed for the ELISA quality-control material, supporting the interpretation that the disagreement reflects systematic differences in method response rather than insufficient ELISA repeatability. Such behavior is well documented when immunoassays are compared with chromatographic methods for complex mycotoxin mixtures, including ergot alkaloids and other structurally diverse mycotoxins in cereals and feed. <a href="javascript:void(0);">(28,30,33)</a> These studies consistently report matrix- and profile-dependent bias in ELISA relative to LC-MS/MS, arising from differential antibody cross-reactivity and the summed immunoreactivity response of the assay, because ELISA produces an immunoreactivity-weighted total that depends on antibody affinity toward individual alkaloids and their relative abundance in the sample, whereas UHPLC-MS/MS quantifies compound-specific concentrations of each alkaloid and its epimers using compound-specific transitions and response factors, and total ergot alkaloid concentration is calculated as the sum of these targeted analytes. In contrast, ELISA produces an immunoreactivity-weighted total, determined by antibody affinity toward individual alkaloids and their competitive binding behavior within the assay format. Consequently, agreement between ELISA and UHPLC-MS/MS depends not only on total concentration, but also on the relative distribution of individual alkaloids within each sample. Although differences in extraction solvent composition and pH may influence ergot alkaloid recovery, stability, and epimeric distribution, each method was applied according to its intended analytical workflow. The ELISA was performed using the manufacturer-recommended acidic methanol/water extraction, whereas UHPLC-MS/MS was performed using the validated acetonitrile/aqueous ammonium carbonate extraction procedure. Therefore, the comparison reflects the performance of the complete methods as used in practice, rather than an isolated comparison of extraction solvents. The sequential extraction experiment further showed that repeating the ELISA extraction under the same kit conditions did not substantially increase the ELISA-measured response, supporting the adequacy of the kit extraction within the ELISA workflow while recognizing that it does not directly compare acidic and ammonium carbonate-based extraction conditions. Previous ELISA-LC-MS/MS comparison studies for ergot alkaloids have reported both under- and overestimation depending on assay design and matrix, including differences in alkaloid profile, cereal type, and antibody cross-reactivity. Turner et al. observed substantial overestimation of ergot alkaloids by ELISA relative to HPLC in rye field samples, attributed to strong antibody cross-reactivity toward alkaloids prevalent in rye matrices. Similar profile-dependent bias has also been reported in interlaboratory and method-comparison studies, <a href="javascript:void(0);">(33)</a> demonstrating that this behavior is not unique to a single data set.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/J_Agric_Food_Chem_2026_74_25_20015_20027_Figure_6_Bland_Altman_plot_comparing_ELISA_and_UHPLC_MS_MS_measurements_of_total_ergot_alkaloids_1be40cce50.jpeg" alt="J. Agric. Food Chem. 2026, 74, 25, 20015–20027: Figure 6. Bland-Altman plot comparing ELISA and UHPLC-MS/MS measurements of total ergot alkaloids for wheat and durum wheat cargo samples (n = 102)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_J_Agric_Food_Chem_2026_74_25_20015_20027_Figure_6_Bland_Altman_plot_comparing_ELISA_and_UHPLC_MS_MS_measurements_of_total_ergot_alkaloids_1be40cce50.jpeg 230w," sizes="100vw" width="1437" height="977"></p><p>This study provides a comprehensive evaluation of a commercially available ELISA for total ergot alkaloids, using wheat samples as the primary application matrix, and integrates performance metrics with mechanistic insights into method behavior. While the assay demonstrated strong repeatability, calibration stability, and robustness to procedural factors such as test portion mass and extraction conditions, systematic differences relative to UHPLC-MS/MS were consistently observed. By linking these differences to compound-specific antibody cross-reactivity and matrix-dependent signal modulation, this work clarifies that ELISA-derived results reflect an immunochemically weighted response rather than a stoichiometric total. This distinction is critical for the appropriate interpretation of ELISA data and supports its use within tiered analytical strategies as a rapid screening tool, complemented by UHPLC-MS/MS for confirmatory quantification and regulatory decision-making.</p></div>

Comparative Evaluation of Total Ergot Alkaloids Determination by ELISA and UHPLC-MS/MS in Wheat: Implications of Cross-Reactivity and Matrix Effects

<p><strong>In the week of July 6, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Waters Corporation: Characterizing Next-Generation Antibody Therapeutics: LC Strategies for Multispecifics, ADCs &amp; AOCs</strong></h5><ul><li>Tu, 7.7.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6317">Registration</a></li></ul><p>In this webinar, we share a pragmatic characterization roadmap using LC-based methods across intact, subunit, and peptide-mapping workflows.</p><h5><strong>2. Separation Science: Advances in Antibiotic Extraction from Water: An Automated SPE Approach</strong></h5><ul><li>We, 8.7.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6319">Registration</a></li></ul><p>Streamline water analysis workflows and gain practical insights for modern environmental monitoring.</p><h5><strong>3. SCIEX: Analysis of per- and polyfluoroalkyl substances (PFAS) in food substances using LC-MS/MS solutions</strong></h5><ul><li>We, 8.7.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6322">Registration</a></li></ul><p>Master PFAS analysis in food safety. Learn how SCIEX LC-MS/MS solutions and QTRAP workflows overcome analytical challenges to ensure accurate and compliant result confirmation.</p><h5><strong>4. Wiley: From liquids to solids: expand what your UV-Vis-NIR Spectrometer can do</strong></h5><ul><li>We, 8.7.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6367">Registration</a></li></ul><p>Discover how the Agilent Cary UV-Vis-NIR spectrometer, together with Specac accessories, enables reliable analysis of liquids, powders, films, pellets, and highly scattering solid samples.</p><h5><strong>5. Thermo Fisher Scientific: Advanced tools to maximize your sample throughput with Chromeleon Software and Vanquish UHPLC</strong></h5><ul><li>Th, 9.7.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6326">Registration</a></li></ul><p>Learn how Chromeleon software and the Vanquish Duo UHPLC system streamline sample analysis, automate routine workflows, increase throughput, and reduce errors through practical demonstrations.</p><h5><strong>6. Bruker: 4D-Metabolomics™ in Action: Real-World Strategies for Deep Discovery and Confident Annotation</strong></h5><ul><li>Th, 9.7.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6328">Registration</a></li></ul><p>Explore how next-generation mass spectrometry technologies, ion mobility, and computational tools are helping researchers move from complex datasets to confident insight.</p><h5><strong>7. Waters Corporation: Cracking the Code on Nitrogen Methods</strong></h5><ul><li>Th, 9.7.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6327">Registration</a></li></ul><p>Learn the fundamentals of nitrogen analysis for wastewater testing. Explore analytical methods, QA/QC, sample handling, compliance reporting, and troubleshooting for accurate environmental monitoring.</p><h5><strong>8. SelectScience: Next-generation metabolomics: From annotation to discovery at scale</strong></h5><ul><li>Th, 9.7.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6281">Registration</a></li></ul><p>Explore how next-generation mass spectrometry, ion mobility, and advanced computational tools accelerate confident small-molecule annotation and discovery in metabolomics.</p><h5><strong>9. SCIEX: LC-MS/HRMS Approaches for Monitoring Organic Micropollutants in Wastewater and Environmental Systems</strong></h5><ul><li>Fr, 10.7.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6370">Registration</a></li></ul><p>Discover how advanced LC-MS/HRMS workflows enable trace-level detection of organic micropollutants, suspect and non-target screening, and environmental monitoring of wastewater systems.</p>

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<p><strong>Advances in metabolomics and lipidomics are generating unprecedented volumes of complex data, but confident identification and biological interpretation of small molecules remain significant challenges. Limitations in annotation, data dimensionality, and reproducibility can slow discovery and reduce confidence in downstream decision‑making across clinical, translational, and applied research.</strong></p><p>In this live broadcast event, leading experts explore how next‑generation mass spectrometry technologies, ion mobility, isotope‑based filtering, and computational tools are helping researchers move from complex datasets to confident insight. Through two short, focused scientific talks, speakers will share real‑world examples of how multi‑dimensional data and advanced workflows are being used to improve metabolite and lipid annotation, accelerate small‑molecule discovery, and enable more robust and reproducible analyses.</p><p>Broadcast as a single, high‑impact session and presented in partnership with Bruker, this webinar provides practical and conceptual guidance for scientists looking to push the boundaries of metabolomics and lipidomics research using modern analytical platforms such as timsMetabo™.</p><h5><strong>This broadcast is designed for:</strong></h5><ul><li>Scientists using mass spectrometry in metabolomics, lipidomics, and small‑molecule analysis</li><li>Researchers in clinical, translational, biopharmaceutical, and toxicology settings</li><li>Teams working in biomarker discovery, exposomics, and pathway analysis</li><li>Lab leaders evaluating next‑generation MS and ion mobility platforms</li></ul><blockquote><p><strong>Speaker: </strong>Dr. Michael Witting (Deputy Head Metabolomics and Proteomics Core &amp; Executive Manager Metabolomics, Helmholtz Munich)</p><p><strong>Speaker:</strong> Prof. Pieter Dorrestein (Skaggs School of Pharmacy and Pharmaceutical Sciences, Departments of Pharmacology and Pediatrics)</p><p><strong>Speaker: </strong>Pelle Simpson (Principal Scientist, Enveda)</p><p><strong>Moderator</strong>: Cameron Smith-Craig (Pharma and Applied Sciences Editor, SelectScience)</p></blockquote>

Bruker: Next‑generation metabolomics: From annotation to discovery at scale

Next‑generation metabolomics: From annotation to discovery at scale

Advance small-molecule discovery with Bruker's next-gen metabolomics. Learn how ion mobility and timsMetabo™ improve metabolite and lipid annotation

Next-Generation Metabolomics and Lipidomics Discovery

<p><strong>Modern biopharma pipelines are rapidly expanding beyond conventional monoclonal antibodies into multi‑specific formats and bioconjugates such as antibody–drug conjugates (ADCs) and antibody–oligonucleotide conjugates (AOCs). While these modalities unlock new therapeutic possibilities, their structural complexity significantly increases analytical demands. Molecular assembly, chain pairing, size, charge, and hydrophobic heterogeneity, post-translational modifications, and stability can all vary in subtle yet clinically meaningful ways. &nbsp;</strong></p><p>In this webinar, we present a practical, end‑to‑end characterization framework that connects LC-based strategies across structural levels—intact, subunit, and peptide—and shows how orthogonal separations support confident decisions from development through QC transfer. Topics include approaches to verify structural integrity, reveal mispaired or incorrectly assembled species, monitor size variants and aggregation, assign charge variants, and apply fit‑for‑purpose workflows to conjugated antibodies where additional heterogeneity must be controlled.</p><p>Attendees will leave with a clear “analytical toolbox” perspective—understanding which techniques to apply, what questions each method answers, and how to assemble robust characterization strategies for emerging antibody modalities.</p><h4><strong>What you'll learn</strong></h4><ul><li>How multispecific formats and conjugates change analytical strategy vs. traditional mAbs</li><li>How LC separates and quantifies variants, while LC–MS enables molecular confirmation</li><li>How to align intact, subunit, and peptide workflows to specific analytical questions</li><li>How conjugation introduces new heterogeneity—and how to manage it effectively (ADC/AOC)</li></ul><h4><strong>Why attend?</strong></h4><p>As antibody therapeutics evolve from conventional mAbs to multi‑specific architectures and bioconjugates (ADCs and AOCs), analytical teams face complexity—more variants, more heterogeneity, and a greater need for orthogonal evidence to support confident development and control strategies.</p><p>In this webinar, we share a pragmatic characterization roadmap using LC-based methods across intact, subunit, and peptide‑mapping workflows—showing how each layer answers different questions, and how to assemble them into a cohesive package.</p><h4><strong>Key takeaways include:</strong></h4><ul><li>Selecting LC/LC‑MS approaches for size, charge, and hydrophobic variants,</li><li>Detecting mispaired or incorrectly assembled multi‑specific species</li><li>Handling the added complexity of conjugated antibodies (ADC/AOC) within fit‑for‑purpose workflows</li></ul><p>Ideal for scientists in analytical development, CMC, and QC supporting next‑generation antibody therapeutics.</p><h4><strong>Who should attend?</strong></h4><p>Analytical scientists in biopharma R&amp;D, process development, and QC working with advanced antibody modalities.</p><p><i><strong>You can select the language of the content in the top right corner of your screen. This webinar will be delivered in English.</strong></i></p><blockquote><p><strong>Speaker:</strong> Ali Tiss, Ph.D., MSc., Eng. (Chat) (Chemistry Market Development Manager, Biopharma &amp; Cell and Gene Therapies, Waters Corporation)</p><p>Ali joined Waters in 2021 and has since supported colleagues and users in the biomolecule separation field (proteins, peptides, Abs, conjugates, Cell &amp; Gene Therapy products). Ali completed his Ph.D. in biochemistry and structural biology at the Mediterranean University (CNRS, Marseille, France), where he contributed to the R&amp;D of the anti-obesity drug, Xenical®, developed by Roche Ltd. Then, he earned over two decades of research experience in biochemistry, liquid chromatography, mass spectrometry, and proteomics, focusing on detecting and characterizing biomarkers related to human diseases such as cancer, diabetes, and obesity. Ali is passionate about supporting customer business and innovative applications.</p></blockquote>

Characterizing Next-Generation Antibody Therapeutics: LC Strategies for Multispecifics, ADCs & AOCs

In this webinar, we share a pragmatic characterization roadmap using LC-based methods across intact, subunit, and peptide‑mapping workflows

LC Strategies for Multispecifics, ADCs & AOCs

<p><strong>The presence of antibiotics in aquatic environments is a growing analytical challenge, with trace-level contamination posing significant risks to ecosystems and contributing to antimicrobial resistance. Accurate, reliable and high-throughput sample preparation is therefore essential for effective water quality monitoring.</strong></p><p>This webinar will demonstrate the use of the Biotage® PrepXpert-8 automated solid-phase extraction (SPE) system for the simultaneous extraction and enrichment of multiple antibiotic classes, including fluoroquinolones, tetracyclines, sulfonamides and macrolides from water matrices.</p><p>We will cover method development considerations, sorbent selection and the advantages of full automation in reducing operator variability and improving reproducibility. Quantitative data and method performance metrics will be presented and discussed.</p><p>Whether you are new to SPE or looking to streamline existing workflows, this webinar offers practical insights for modern environmental water analysis.</p><p><strong>By attending this presentation, you will:</strong></p><ul><li>Gain practical knowledge of automated SPE method development for extracting multiple antibiotic classes from water matrices using the Biotage® PrepXpert-8.</li><li>Learn key sorbent selection strategies and see real quantitative recovery data to support your own method implementation.</li><li>Leave equipped to streamline your sample preparation workflows and improve reproducibility in water analysis.</li></ul><h5><strong>Who should attend?</strong></h5><p>This webinar is ideal for analytical and environmental chemists, water quality scientists and laboratory managers working in environmental monitoring, regulatory compliance, or pharmaceutical research who are involved in trace-level contaminant analysis.</p><h5><strong>What you need to know:</strong></h5><p><strong>Start times:</strong></p><ul><li><strong>Broadcast 1:</strong> 8 am BST (London/Lisbon) / 9 am CEST (Paris/Berlin) / 12:30 pm IST (New Delhi) / 3 pm CST (Beijing) / 4 pm JST (Tokyo)</li><li><strong>Broadcast 2: </strong>8 am CDT (Chicago) / 9 am EDT (New York) / 2 pm BST (London/Lisbon) / 3 pm CEST (Paris/Berlin)</li><li><strong>Broadcast 3: </strong>10 am PDT (Los Angeles) / 12 pm CDT (Chicago) / 1 pm EDT (New York)</li></ul><p><strong>Duration:</strong> 1 hour</p><blockquote><p><strong>Presenter:</strong> Vainius Skukauskas (Applications Chemist, Biotage EMEA Application Laboratory, Cardiff, UK)</p><p>Dr. Vainius Skukauskas is an Applications Chemist at the Biotage EMEA Application Laboratory in Cardiff, UK. With a Ph.D. in chemistry and extensive expertise in analytical method development, he specializes in developing and optimizing automated sample preparation workflows across a wide range of industries, with a particular focus on the environmental and food sectors. He works closely with customers to translate complex methods into robust, high-throughput solutions, and regularly delivers technical demonstrations, training, and workshops across EMEA.</p></blockquote>

Separation Science: Advances in Antibiotic Extraction from Water - An Automated SPE Approach

Advances in Antibiotic Extraction from Water: An Automated SPE Approach

Advance water quality monitoring. Discover automated SPE method development and sorbent selection for trace-level antibiotic extraction with Biotage

Automated SPE Approach for Antibiotic Extraction from Water

<p><strong>Join us for an insightful webinar on the analysis of Per- and Polyfluoroalkyl Substances (PFAS) in food substances using advanced LC-MS/MS technology. This session will cover the importance of PFAS testing in food safety, key analytical challenges, sample preparation strategies, method development approaches, and best practices for accurate and sensitive detection using LC-MS/MS systems. Experts will also discuss workflow optimization, regulatory considerations, and real-world applications across different food matrices, helping laboratories and researchers strengthen their PFAS analysis capabilities.</strong></p><h5><strong>Key highlights:</strong></h5><ul><li>PFAS in food and it’s regulation around the world</li><li>Analytical challenges &amp; SCIEX LC-MS/MS solutions</li><li>QTRAP workflow to confirm the true or false results in food samples<br>&nbsp;</li></ul><blockquote><p><strong>Expert:</strong> Kankan Jana (Application Support Specialist, SCIEX)</p></blockquote>

SCIEX: Analysis of per- and polyfluoroalkyl substances (PFAS) in food substances using LC-MS/MS solutions

Analysis of per- and polyfluoroalkyl substances (PFAS) in food substances using LC-MS/MS solutions

Advance food safety testing. Discover SCIEX LC-MS/MS methods and QTRAP workflows for accurate detection and regulatory compliance of PFAS in food

PFAS Analysis in Food Safety: SCIEX LC-MS/MS Solutions

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Leverage unique capabilities and powerful tools to streamline sample analysis. Are you using your Thermo Scientific Vanquish UHPLC systems to their fullest potential?</strong></p><p>Join this webinar to discover the multiple tools in Thermo Scientific Chromeleon software that drive sample throughput from acquisition to data processing and deliver reduced errors through advanced automation.</p><h4><strong>Why attend</strong></h4><p>Uncover the Chromeleon software features that drive sample throughput and deliver unique capabilities in combination with the <strong>Thermo Scientific Vanquish Duo UHPLC system</strong>. In this webinar, you will see how Chromeleon software can help:</p><ul><li>Increase sample throughput through streamlined workflows</li><li>Leverage the full capabilities of the Vanquish Duo UHPLC system&nbsp;</li><li>Deliver automation to common analyses to reduce errors</li></ul><p>Through practical examples and live software demonstrations, attendees will learn how to improve day-to-day laboratory efficiency and reduce errors.</p><h4><strong>What you will learn</strong></h4><p>By attending, participants will be able to:</p><ul><li>Streamline analysis in Chromeleon software from acquisition to result</li><li>Understand and leverage the unique capabilities of Vanquish Duo UHPLC systems &nbsp;</li><li>Drive automation in common workflows to reduce errors</li></ul><p>Save your spot to discover practical ways to optimize Vanquish UHPLC system workflows with Chromeleon software.</p><p><i>Cannot attend on July 9? Please register anyway to automatically receive the link to the recording.</i></p><blockquote><p><strong>Presenter: </strong>Darren Barrington-Light (Senior Manager, Product Marketing, Thermo Fisher Scientific)</p><p>Darren Barrington-Light’s background includes over 25 years of experience in the field of analytical chemistry, chromatography hardware and computer software. Having worked in chromatography throughout his career, Darren has a unique understanding of the requirements of organizations that rely on chromatography data to manage their day to day operations. As capabilities have advanced, he has worked with clients across multiple industries to show them how software solutions can help them to improve their day to day operations and drive fast, informed decisions.</p></blockquote></div></div></div>

Thermo Fisher Scientific: Advanced tools to maximize your sample throughput with Chromeleon Software and Vanquish UHPLC

Advanced tools to maximize your sample throughput with Chromeleon Software and Vanquish UHPLC

Discover how Chromeleon software optimizes Vanquish UHPLC workflows with automation, higher throughput, and reduced errors from acquisition to results.

Sample throughput with Chromeleon and Vanquish UHPLC

<h4><strong>A Practical Webinar for Wastewater Testing and Compliance Professionals</strong></h4><p><strong>Nitrogen analysis plays a critical role in wastewater treatment, compliance monitoring, and nutrient management, yet selecting the right method and interpreting results correctly can be complex. In this webinar, we’ll break down the most common nitrogen parameters, including ammonia, nitrate/nitrite, TKN, and total nitrogen, and explain how these measurements support both regulatory reporting and process control.</strong></p><p>Attendees will gain practical insight into commonly used analytical methods, sample handling and QA/QC best practices, and how to avoid common reporting and interpretation mistakes. We’ll also review real-world scenarios involving nitrification, denitrification, permit compliance, and troubleshooting elevated nitrogen results.</p><p>Whether you work in a laboratory, wastewater treatment facility, or environmental compliance role, this session will provide the clarity and confidence needed to improve data quality, optimize operations, and make informed nitrogen testing decisions.</p><h4><strong>Key takeaways</strong></h4><ul><li>Understand key nitrogen methods and reporting requirements</li><li>Improve testing accuracy with better QA/QC and sample handling</li><li>Gain practical insights for compliance, troubleshooting, and process optimization</li></ul><h4><strong>Who should attend</strong></h4><p>This webinar is designed for you if you are:</p><ul><li>Analytical Chemist</li><li>QA Manager</li><li>Process Engineer</li><li>Wastewater Operator</li></ul><blockquote><p><strong>Speaker:</strong> Sam Johnson (Inorganic Chemist, Waters ERA)</p><p>Sam has been with Waters ERA for 11 years, specializing in the manufacturing and analytical verification of inorganic standards and reagents.</p><p>He holds a Bachelor of Science in Geology with a minor in Watershed Science from Colorado State University in Fort Collins.</p><p>Throughout his career, Sam has supported the environmental testing industry by providing solutions for wastewater, drinking water, and soil testing laboratories.</p><p><strong>Speaker: </strong>Christy Abbas, PhD (Sr. Product Marketing Manager, Waters ERA)</p><p>Christy has been with Waters ERA for six and a half years. Her career spans roles at an instrument manufacturer, a biopharmaceutical laboratory, and a mining company. She earned her PhD in Applied Chemistry from the Colorado School of Mines.</p><p>Throughout her career, Christy has supported the nuclear, pharmaceutical, and chemical industries, as well as academia. Drawing on her background in analytical chemistry, product development, marketing, and sales, she is focused on expanding Waters ERA’s product portfolio to better meet evolving customer needs.</p></blockquote>

Cracking the Code on Nitrogen Methods

Discover practical approaches to nitrogen analysis in wastewater, including testing methods, QA/QC, compliance requirements, and process optimization.

Cracking Code on Nitrogen Methods

<p><strong>Advances in metabolomics and lipidomics are generating unprecedented volumes of complex data, but confident identification and biological interpretation of small molecules remain significant challenges. Limitations in annotation, data dimensionality, and reproducibility can slow discovery and reduce confidence in downstream decision making across clinical, translational, and applied research.</strong></p><p>In this live broadcast event, leading experts explore how next generation mass spectrometry technologies, ion mobility, and computational tools are helping researchers move from complex datasets to confident insight. Through three short, focused scientific talks, speakers will share real world examples of how multi dimensional data and advanced workflows are being used to improve metabolite and lipid annotation, accelerate small molecule discovery, and enable more robust and reproducible analyses.</p><h4><strong>Who Should Attend</strong></h4><ul><li>Untargeted metabolomics and natural products researchers focused on spectral annotation and structure elucidation</li><li>Microbiome, exposomics, and environmental health scientists&nbsp;</li><li>Drug discovery scientists using AI/ML and computational chemistry</li><li>Core facility managers and method developers building reproducible, multi-dimensional LC-MS/MS workflows</li><li>Cheminformatics and computational scientists working with spectral repositories and ML-based metabolite prediction</li><li>Lab leaders evaluating next-generation ion mobility MS platforms for 4D-metabolomics and 4D-lipidomics</li></ul><h4><strong>Key Learning Objectives</strong></h4><ul><li>How to boost identification confidence and speed using timsMetabo™, isotope‑based filtering, and MassQL</li><li>Applying multi‑dimensional data (including ion mobility and CCS) to improve metabolite annotation</li><li>Tips for accelerating small‑molecule discovery and lipidomics workflows with robust, reproducible approaches</li><li>Navigating current bottlenecks in non‑targeted and targeted metabolomics and lipidomics</li></ul><blockquote><p><strong>Speaker: </strong>PD Dr. Michael Witting, Deputy Head Metabolomics and Proteomics Core &amp; Executive Manager Metabolomics, Helmholtz Munich, Munich, Germany</p><p><strong>Speaker: </strong>Pieter Dorrestein, Professor, Skaggs School of Pharmacy and Pharmaceutical Sciences Departments of Pharmacology and Pediatrics, UC San Diego, San Diego, CA, USA</p><p><strong>Speaker: </strong>Pelle Simpson, Principal Scientist, Enveda, Boulder, CO, USA</p></blockquote>

Bruker: 4D-Metabolomics™ in Action: Real‑World Strategies for Deep Discovery and Confident Annotation

4D-Metabolomics™ in Action: Real‑World Strategies for Deep Discovery and Confident Annotation

Next generation mass spectrometry technologies, ion mobility, and computational tools are helping researchers move from complex datasets to confident insight. 

4D-Metabolomics™ in Action

<p><strong>From high-throughput extraction and anti-clogging strategies to up-concentration and LC-MS/MS analysis.</strong></p><p>PFAS analysis in aqueous matrices requires robust sample preparation, reliable clean-up, and consistent recoveries - especially as ultra-short-chain PFAS such as TFA are gaining increasing regulatory attention.</p><p>In this Velaris webinar, experts from PromoChrom and LCTech will present automated SPE-based workflows for PFAS analysis in drinking water, environmental water, wastewater, and other aqueous matrices compliance with methods such as EPA 533, EPA 537.1, EPA 1633, and EU Method 17892.</p><p>The session will cover fully automated SPE with the PromoChrom SPE-03 platform, anti-clogging solutions for challenging samples, optimized PFAS SPE cartridges, and Velaris solutions for the extraction of ultra-short-chain PFAS.</p><p>The webinar will also introduce the D-EVA vacuum concentrator for unattended sample concentration and show how automated sample preparation can support high throughput, reproducibility, low background levels.</p><blockquote><p><strong>Presenter: </strong>Keith Keegan (Lead Applications Engineer, PromoChrom)</p><p>Keith Keegan will focus on the PromoChrom SPE-03 platform for fully automated SPE, workflow efficiency, anti-clogging strategies, real-world laboratory use, and future-oriented tools such as real-time monitoring and remote fleet management.</p></blockquote><blockquote><p><strong>Presenter: </strong>Dr. Suman Kharel (Application Specialist, LCTech)</p><p>Suman Kharel will present optimized SPE cartridges for PFAS analysis, Velaris solutions for ultra-short-chain PFAS, performance data for aqueous matrices, and the use of D-EVA for unattended concentration before LC-MS/MS analysis.</p></blockquote>

GERSTEL: Automated SPE Sample Preparation for PFAS and Ultra-Short-Chain PFAS in Aqueous Matrices

Automated SPE Sample Preparation for PFAS and Ultra-Short-Chain PFAS in Aqueous Matrices

Master automated PFAS sample prep. Learn SPE-03 extraction techniques and D-EVA concentration for ultra-short-chain PFAS in compliance with EPA 1633

Automated SPE for Ultra-Short-Chain PFAS in Water

<h4><strong>Your UV-Vis-NIR can do far more than measure liquids</strong></h4><p><strong>Analysis of solid samples and heterogeneous samples by UV-Vis and UV-Vis-NIR spectroscopies is possible with suitable sample accessories.</strong></p><p>In this webinar, experts from Agilent and Specac will demonstrate how the Cary UV-Vis-NIR system can analyze a range of samples, from liquids to powders, films, pellets, and highly scattering solids.</p><p>Illustrated with real examples, we will show how diffuse reflectance spectroscopy (DRS) and remote sampling with fiber optic probes can be used for bandgap determination, optical property characterization, photocatalysis research, and in situ reaction monitoring.</p><p>We will highlight the Harrick Praying Mantis for operando reaction monitoring and the FiberMate2 for remote studies in more complex experimental set ups.</p><h5><strong>What You'll Learn</strong></h5><p>See how Agilent Cary UV-Vis-NIR system, paired with the right accessories, handles liquids, powders, pellets, and films without compromise. Expert-led case studies cover bandgap determination, photocatalysis, and operando monitoring under real conditions.</p><h5><strong>Key Takeaways &nbsp;</strong></h5><ul><li>Extend your UV-Vis system to solids (powders, pellets, thin films) without buying new instrumentation</li><li>Apply Diffuse reflectance (DRS) for high-sensitivity analysis of micro-quantities with minimal sample prep</li><li>Monitor different temperature measurement&nbsp;</li><li>Enable angle-resolved solid measurements for thin films and surface treatments.&nbsp;</li><li>Integrate fiber optic probes for remote, in situ monitoring</li></ul><h5><strong>Who Should Attend</strong></h5><p>Academic researchers in materials science, photonics, optical engineering, or catalysis who want to get more analytical range from their existing UV-Vis setup.&nbsp;</p><blockquote><p><strong>Presenter: </strong>Dr. Thibault Brulé (Product Specialist Molecular Spectroscopy, AGILENT TECHNOLOGIES)</p><p>PhD in Physics since 2014, I swapped lab benches for real-world challenges. Over 7 years, I honed my skills in technical and application support, focusing on Raman spectroscopy, before joining Agilent end of 2024, where I now help customers navigate the world of molecular spectroscopy (UV/Vis/NIR, FTIR and Raman). My mission: turn complex analytical problems into clear, effective solutions—with a dash of fun whenever possible!</p><p><strong>Presenter:&nbsp;</strong>Bisirat Araya (Applications Scientist, SPECAC LTD)</p><p>Bisirat holds a B.A. and M.S. in Chemical Engineering from Manhattan University and is an Applications Scientist at Specac-Harrick with over 12 years of experience at Specac and Harrick Scientific. She specialises in technical customer support, sample evaluations, equipment setup, and technical documentation.</p><p>Bisirat collaborates with customers to optimise equipment performance and works with applications and engineering teams to develop new products.</p></blockquote>

Agilent Technologies: From liquids to solids: expand what your UV-Vis-NIR Spectrometer can do

From liquids to solids: expand what your UV-Vis-NIR Spectrometer can do

In this webinar, experts from Agilent and Specac will demonstrate how the Cary UV-Vis-NIR system can analyze a range of samples, from liquids to powders,

What your UV-Vis-NIR Spectrometer can do

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Join us for an insightful webinar exploring how advanced LC-MS/HRMS workflows are enabling sensitive detection and comprehensive analysis of trace organic micropollutants in wastewater and Environmental systems.</strong></p><p>The session will highlight analytical strategies for identifying emerging contaminants, monitoring their transformation and fate during wastewater treatment, and addressing challenges associated with complex environmental matrices. Attendees will also gain insights into high-resolution screening workflows, environmental risk assessment, and future perspectives in environmental monitoring.</p><h5><strong>Key highlights:</strong></h5><ul><li>Trace-level detection and monitoring of organic micropollutants using LC-MS/HRMS</li><li>Understanding the fate, transport, transformation, and removal of contaminants in wastewater treatment plants and aquatic environments</li><li>Targeted, suspect, and non-target screening approaches for identification of known and unknown contaminants</li></ul><blockquote><p><strong>Presenter: </strong>Dr. Vellanki, Bhanu Prakash (Professor at IIT Roorkee , Specialization : Environmental Engineering)</p><p>Dr. Bhanu Prakash Vellanki is a faculty member at IIT Roorkee specializing in Environmental Engineering and sustainable water treatment technologies. He was one of the 9 global winners of the Clean Technology Start-up Challenge in 2022 among 127 applicants from 21 different countries and Awarded with Manush Labs and Aditya Birla Group’s Hindalco on sustainability initiatives.</p></blockquote></div></div></div>

SCIEX: LC-MS/HRMS Approaches for Monitoring Organic Micropollutants in Wastewater and Environmental Systems

LC-MS/HRMS Approaches for Monitoring Organic Micropollutants in Wastewater and Environmental Systems

Learn how LC-MS/HRMS supports trace organic micropollutant analysis, wastewater monitoring, and targeted, suspect, and non-target screening.

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