ISC 2026 Short Course 1 - Modern HPLC Separation in Theory and Practice
International Symposium on Chromatography: ISC 2026 Short Course 1 - Modern HPLC Separation in Theory and Practice
ISC 2026
The International Symposium on Chromatography (ISC) represents the oldest conference series on separation science. ISC symposia have been organised since 1956 in each even year. ISC is one of the premier meetings series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.
The major focus of the symposium will be on the impact of chromatography and separation science to meet the needs of the pharmaceutical, environmental, food and health industry, as well as science and medicine. The symposium programme will reflect these themes and highlight new challenges and emerging opportunities for the science and marketing of separation and detection systems and methods.
As an integral part of the scientific programme of lectures and poster sessions, an international exhibition and vendor seminars on instrumentation and services for chromatography, separation science and mass spectrometry will be organised. ISC 2026 will provide the perfect forum for attendees from academia, industry and government research institutions for scientific exchange and networking.
ISC 2026 SYMPOSIUM KEY DATES
6-10 SEPTEMBER 2026
- 15 January 2026: Opening of Early-bird Registration & Abstract submission
- 11 March 2026: Deadline for abstract submission (orals)
- 21 April 2026: Sending acceptance letters (orals)
- 15 May 2026: Deadline of early-bird registration and payment & Author registration deadline (orals)
- 1 June 2026: Preliminary detailed program on web
- 1 June 2026: Deadline for abstract submission (posters)
- 16 June 2026: Sending acceptance letters (posters)
- Authors who submit their abstracts with a poster preference by 5 May 2026 will be notified of the outcome by 12 May 2026 and will have the opportunity to register at the early-bird rate until 15 May 2026.
- 30 June 2026: Author registration deadline (posters)
- 30 July 2026: Deadline for abstract submission (late-breaking posters)
- without the possibility of competing for the Best Poster Award
- 11 August 2026: Sending acceptance letters (late-breaking posters)
- 18 August 2026: Author registration deadline (late-breaking posters)
- 25 August 2026: Deadline for late registration & payment
- 6-10 September 2026: Symposium
REGISTRATION
ABSTRACT SUBMISSION
SCIENTIFIC PROGRAM
SHORT COURSES
SC 1 Modern HPLC Separation in Theory and Practice
🗓️ SATURDAY, 5 SEPTEMBER 2026
🕗 9:30 – 17:00
High-performance liquid chromatography (HPLC) is one of the most powerful and widely applied separation techniques in modern analytical chemistry. This short course provides a comprehensive introduction to contemporary HPLC, combining fundamental chromatographic theory with practical guidance relevant to everyday analytical practice.
In the first part, we will focus on the overview of key separation principles, including compound retention, selectivity, separation efficiency, and gradient elution. We will cover HPLC instrumentation, column technologies, and stationary phase chemistry, with emphasis on how these factors influence separation efficiency and selectivity, but also method robustness.
Subsequently, the course introduces different separation modes and recent developments in liquid chromatography, including ultra-high-performance liquid chromatography (UHPLC), high-temperature LC, supercritical fluid chromatography (SFC), and two-dimensional liquid chromatography (2D-LC). Practical strategies for method development and optimization are emphasized throughout the course, enabling participants to apply modern HPLC solutions effectively in their own work.
Participants will gain a solid understanding of HPLC theory, practical method development, and an overview of current trends in UHPLC, SFC, and multidimensional LC. The course concludes with an interactive knowledge quiz, offering participants an opportunity to test their understanding and compete for a copy of the book Modern HPLC Separations in Theory and Practice, authored by the course instructors. The course also offers a unique opportunity to meet all the book authors in person and to have your own book copy signed.
Lecturers
- Lucie Nováková
- Lucie Nováková is a Full Professor in Analytical Chemistry at the Charles University, Faculty of Pharmacy in Hradec Králové, Czech Republic. Her research is focused on separation techniques, including UHPLC, SFC and their coupling to mass spectrometry. She is involved in a wide scope of research projects in pharmaceutical analysis, doping control, plant analysis, and bioanalytical methods. She authored the book on HPLC theory and practice in Czech and in English and ten book chapters. She published almost 180 peer-reviewed scientific articles with more than 6600 citations and an h-index of 44. She is also widely involved in teaching and education activities.
- Jiří Urban
- Jiri Urban received his Ph.D. in 2007 under the supervision of Pavel Jandera at the University of Pardubice, Czech Republic, where he remained until 2016. From 2009 to 2011, he conducted postdoctoral research at the University of California, Berkeley, USA, in the groups of Frantisek Svec and Jean M. J. Fréchet. In 2017, he joined the Department of Chemistry at Masaryk University in Brno, Czech Republic, where he was appointed Associate Professor in 2018 and subsequently became a Full Professor in 2025. His research focuses on the use of multifunctional polymer monoliths for developing new analytical methods applicable to metabolomics and proteomics. He also investigates retention modeling in chromatographic method development and the design of novel instrumental setups for two-dimensional liquid chromatography.
- Petr Česla
- Petr Česla is an Associate Professor and Head of the Department of Analytical Chemistry at the Faculty of Chemical Technology, University of Pardubice, Czech Republic. His research focuses on the development of liquid chromatographic separations, including two-dimensional separations, retention modeling, and optimization procedures. The main application areas of his work include the analysis of naturally occurring plant metabolites, pharmaceuticals, and industrial samples such as surfactants, oligomers and synthetic dyes. He also represents the Czech Republic in the Central European Group of Separation Sciences.
- Michal Douša
- Michal Douša has served as the Head of the Separation Methods Department at the pharmaceutical company Zentiva since 2007. His research focuses on separation techniques, particularly HILIC, chiral separation, and derivatization techniques. He has prior experience in HPLC analysis of soil, food, and feed. He is the author of a book on HPLC theory and practice and one book chapter. He has published over 67 articles, accumulating more than 1039 citations (h-index 20). He is also actively involved in teaching and educational activities, including HPLC and validation training courses. He serves as the Chairman of the Czech Chromatography School association.
