LCMS
More information
WebinarsAbout usContact usTerms of use
LabRulez s.r.o. All rights reserved. Content available under a CC BY-SA 4.0 Attribution-ShareAlike
Author
University of Amsterdam
University of Amsterdam
The University of Amsterdam is one of the largest comprehensive universities in Europe. With some 40,000 students, 6,000 staff, 3,000 PhD candidates, and an annual budget of more than 850 million euros, it is also one of Amsterdam’s biggest employers. There is an inseparable link between the university and its host city. The freedom and creativity with which Amsterdam has long been associated have worked their way into the fabric of the university. This is a place where individuality, authenticity and diversity are celebrated as sources of stimulation, inspiration and innovation. Professional and personal development, challenges and collaboration are undertaken in a versatile and multidimensional (inter)national ecosystem with leading stakeholders. Staff at the UvA make a meaningful contribution to uncovering solutions to some of the world’s most significant problems and to developing the next generation of talent.
Tags
Article
Scientists
Science and research
Video
LinkedIn Logo

Bob Pirok receives LCGC Emerging Leader award

Dr. Bob Pirok receives the 2026 Emerging Leader in Chromatography Award for advances in LC×LC, automated method development and chemometrics at the Van ’t Hoff Institute.
Video placeholder
  • Photo: Van 't Hoff Institute for Molecular Sciences: Bob Pirok receives LCGC Emerging Leader award
  • Video: LabRulez: Bob Pirok: Unsupervised optimization of heart-cut and comprehensive 2D-LC separations (MDCW 2023)

"Education is central to how I approach research"

Analytical scientist Dr Bob Pirok of the Van ‘t Hoff Institute for Molecular Sciences has been awarded the 19th annual ‘Emerging Leader in Chromatography Award’ by LCGC International, a well-known global publisher in the field of separation science. Pirok will receive the award at the upcoming Pittcon 2026 conference held 7-11 March in San Antonio, Texas.

With the Emerging Leader in Chromatography Award, LGCG International recognises the achievements and aspirations of talented early-career separation scientists who have made strides toward the advancement and applications of chromatography techniques. Pirok is honoured for his work in the development of comprehensive two-dimensional liquid chromatography (LC×LC), automated method development, and chemometric data analysis.

Already in his early career, Pirok has emerged as a prominent figure in addressing long-standing challenges in LC×LC, which include method complexity, data interpretation, and experimental optimisation. His research is characterised by close interaction between theory, instrumentation, and application, as well as sustained collaboration with academic and industrial partners. On a mission to transform advanced chromatographic techniques into practical and robust analytical tools, Pirok has authored or co-authored more than 50 peer-reviewed publications and established an internationally visible research program at the interface of separation science, statistics, and artificial intelligence. All this within a few years of receiving his PhD in 2019.

Automated method development

Central to Pirok’s research is the novel modular AutoLC platform, which enables prototype automated method development. With a capacity to run unsupervised (i.e. without human input), it is able to tackle samples of unknown composition by autonomous analysis of (2D-)LC(-MS) data. It is flexible, able to support different optimization strategies and capable of integrating tools developed by scientists in the global chromatographic community.

Accessibility and sustainability are key motivations behind AutoLC, says Pirok: “State-of-the-art separation technology is often overly sophisticated. We aim to make it more robust, automated, and usable beyond a small group of specialists, as efficiently as possible. By providing more detailed and reliable molecular information, such technologies can better inform molecular design and accelerate the development of new materials and applications.

HIMS: From left to right: Bob Pirok, Prof. Peter Schoenmakers, PhD candidate Gerben van Henten, and postdoc researcher Tijmen Bos in the AutoLC lab at the Van ‘t Hoff Institute for Molecular Sciences. The AutoLC workflow builds on concepts originally introduced by Schoenmakers in 1978, which have now been adapted and extended within a modern, automated framework by the AutoLC team. Schoenmakers, who retired in 2022, was awarded the 2023 LCGC Lifetime Achievement Award.HIMS: From left to right: Bob Pirok, Prof. Peter Schoenmakers, PhD candidate Gerben van Henten, and postdoc researcher Tijmen Bos in the AutoLC lab at the Van ‘t Hoff Institute for Molecular Sciences. The AutoLC workflow builds on concepts originally introduced by Schoenmakers in 1978, which have now been adapted and extended within a modern, automated framework by the AutoLC team. Schoenmakers, who retired in 2022, was awarded the 2023 LCGC Lifetime Achievement Award.

He is especially grateful to his students who continuously challenge assumptions and push the technology further. “They show why research and education belong together”, he says. “In fact, education is central to how I approach research. Many of the ideas behind AutoLC grew directly from discussions with students, in the lab, in courses, or while trying to explain complex concepts in a clear and accessible way. Seeing students grow into independent scientists, capable of questioning methods and building new ones, is one of the most rewarding aspects of my work.” 

About Bob Pirok

Bob Pirok (Den Helder, 1987) completed his MSc in analytical chemistry at the University of Amsterdam, followed by doctoral research conducted in close collaboration with industrial partners including Shell, DSM, and Heineken. After earning his PhD in 2019 with Prof. Peter Schoenmakers, Pirok was appointed tenure-track assistant professor at the Van ’t Hoff Institute for Molecular Sciences. There, he co-founded the Chemometrics and Advanced Separations Team (CAST) and was appointed associate professor in 2025.

Pirok is also a visiting researcher at Gustavus Adolphus College in the United States and Stellenbosch University in South Africa. Pirok has received the Shimadzu Young Scientist Award (2015), the Csaba Horváth Young-Scientist Award (HPLC 2017), and the Journal of Chromatography Young-Scientist Award (2018). He is a driving force within the University of Amsterdam’s analytical chemistry programs, where he teaches courses in chemometrics, statistics, and separation science and supervises many MSc and BSc students. Pirok serves on the Early Career Board of Analytical Chemistry and is active in professional networks such as the European Chemistry Thematic Network.

HIMS: Bob Pirok speaking at the Balaton 2025 symposium in Siófok, Hungary, in September 2025HIMS: Bob Pirok speaking at the Balaton 2025 symposium in Siófok, Hungary, in September 2025

University of Amsterdam
LinkedIn Logo
 

Related content

Overcoming Strong Solvent Effects in the Analysis of Vepdegestrant

Applications
| 2026 | Agilent Technologies
Instrumentation
HPLC
Manufacturer
Agilent Technologies
Industries
Pharma & Biopharma

Identification of Double Bond Positions and Relative Acyl Chain Positions in Egg Yolk Phosphatidylcholines Using OAD-TOF System

Applications
| 2026 | Shimadzu
Instrumentation
LC/MS, LC/MS/MS, LC/TOF, LC/HRMS
Manufacturer
Shimadzu
Industries
Food & Agriculture

High Molecular-Weight Polysaccharide Characterization by SEC-MALS Using GTxResolve™ 1000 and 2000 Å SEC Columns

Applications
| 2026 | Waters
Instrumentation
GPC/SEC, Consumables, LC columns
Manufacturer
Waters
Industries
Pharma & Biopharma, Food & Agriculture

Development and Optimization for a Comprehensive LC/MS/MS Method for the Detection of 74 PFAS Compounds

Applications
| 2026 | Agilent Technologies
Instrumentation
LC/MS, LC/MS/MS, LC/QQQ
Manufacturer
Agilent Technologies
Industries
Food & Agriculture

PFAS in Biota: Risk Context & Robust Analytical Solutions

Others
| 2026 | ALS Europe
Instrumentation
Laboratory analysis, LC/MS, LC/MS/MS
Manufacturer
Industries
Environmental
 

Related articles

Why Is It Necessary to Dry the SPE Sorbent Before Elution?
Article | Academy

Why Is It Necessary to Dry the SPE Sorbent Before Elution?

Proper drying of the SPE sorbent is a critical step to achieve high sensitivity, good reproducibility, and maximum analyte recovery, especially for complex sample matrices.
Phenomenex
tag
share
more
 Czech scientists develop a material for producing chemicals from water and sunlight, inspired by human enzymes
Article | Science and research

Czech scientists develop a material for producing chemicals from water and sunlight, inspired by human enzymes

Czech scientists from CATRIN and CEET developed an enzyme-inspired photocatalyst that produces hydrogen peroxide from sunlight, water and oxygen—without toxic solvents or precious metals.
CATRIN
tag
share
more
Oligos Made Easy - Part 3: IP-RP
Article | Science and research

Oligos Made Easy - Part 3: IP-RP

Discover how ion-pair reversed-phase (IP-RP) chromatography enables high-resolution oligonucleotide analysis. Learn about ion-pair selection, method setup, LC-MS compatibility, and robust separation strategies.
KNAUER
tag
share
more
News from LabRulezLCMS Library - Week 08, 2026
Article | Application

News from LabRulezLCMS Library - Week 08, 2026

This week we bring you application notes by Agilent Technologies, KNAUER, Shimadzu and Waters Corporation and presentation by MDCW / Clemson University!
LabRulez
tag
share
more
 

Related content

Overcoming Strong Solvent Effects in the Analysis of Vepdegestrant

Applications
| 2026 | Agilent Technologies
Instrumentation
HPLC
Manufacturer
Agilent Technologies
Industries
Pharma & Biopharma

Identification of Double Bond Positions and Relative Acyl Chain Positions in Egg Yolk Phosphatidylcholines Using OAD-TOF System

Applications
| 2026 | Shimadzu
Instrumentation
LC/MS, LC/MS/MS, LC/TOF, LC/HRMS
Manufacturer
Shimadzu
Industries
Food & Agriculture

High Molecular-Weight Polysaccharide Characterization by SEC-MALS Using GTxResolve™ 1000 and 2000 Å SEC Columns

Applications
| 2026 | Waters
Instrumentation
GPC/SEC, Consumables, LC columns
Manufacturer
Waters
Industries
Pharma & Biopharma, Food & Agriculture

Development and Optimization for a Comprehensive LC/MS/MS Method for the Detection of 74 PFAS Compounds

Applications
| 2026 | Agilent Technologies
Instrumentation
LC/MS, LC/MS/MS, LC/QQQ
Manufacturer
Agilent Technologies
Industries
Food & Agriculture

PFAS in Biota: Risk Context & Robust Analytical Solutions

Others
| 2026 | ALS Europe
Instrumentation
Laboratory analysis, LC/MS, LC/MS/MS
Manufacturer
Industries
Environmental
 

Related articles

Why Is It Necessary to Dry the SPE Sorbent Before Elution?
Article | Academy

Why Is It Necessary to Dry the SPE Sorbent Before Elution?

Proper drying of the SPE sorbent is a critical step to achieve high sensitivity, good reproducibility, and maximum analyte recovery, especially for complex sample matrices.
Phenomenex
tag
share
more
 Czech scientists develop a material for producing chemicals from water and sunlight, inspired by human enzymes
Article | Science and research

Czech scientists develop a material for producing chemicals from water and sunlight, inspired by human enzymes

Czech scientists from CATRIN and CEET developed an enzyme-inspired photocatalyst that produces hydrogen peroxide from sunlight, water and oxygen—without toxic solvents or precious metals.
CATRIN
tag
share
more
Oligos Made Easy - Part 3: IP-RP
Article | Science and research

Oligos Made Easy - Part 3: IP-RP

Discover how ion-pair reversed-phase (IP-RP) chromatography enables high-resolution oligonucleotide analysis. Learn about ion-pair selection, method setup, LC-MS compatibility, and robust separation strategies.
KNAUER
tag
share
more
News from LabRulezLCMS Library - Week 08, 2026
Article | Application

News from LabRulezLCMS Library - Week 08, 2026

This week we bring you application notes by Agilent Technologies, KNAUER, Shimadzu and Waters Corporation and presentation by MDCW / Clemson University!
LabRulez
tag
share
more
 

Related content

Overcoming Strong Solvent Effects in the Analysis of Vepdegestrant

Applications
| 2026 | Agilent Technologies
Instrumentation
HPLC
Manufacturer
Agilent Technologies
Industries
Pharma & Biopharma

Identification of Double Bond Positions and Relative Acyl Chain Positions in Egg Yolk Phosphatidylcholines Using OAD-TOF System

Applications
| 2026 | Shimadzu
Instrumentation
LC/MS, LC/MS/MS, LC/TOF, LC/HRMS
Manufacturer
Shimadzu
Industries
Food & Agriculture

High Molecular-Weight Polysaccharide Characterization by SEC-MALS Using GTxResolve™ 1000 and 2000 Å SEC Columns

Applications
| 2026 | Waters
Instrumentation
GPC/SEC, Consumables, LC columns
Manufacturer
Waters
Industries
Pharma & Biopharma, Food & Agriculture

Development and Optimization for a Comprehensive LC/MS/MS Method for the Detection of 74 PFAS Compounds

Applications
| 2026 | Agilent Technologies
Instrumentation
LC/MS, LC/MS/MS, LC/QQQ
Manufacturer
Agilent Technologies
Industries
Food & Agriculture

PFAS in Biota: Risk Context & Robust Analytical Solutions

Others
| 2026 | ALS Europe
Instrumentation
Laboratory analysis, LC/MS, LC/MS/MS
Manufacturer
Industries
Environmental
 

Related articles

Why Is It Necessary to Dry the SPE Sorbent Before Elution?
Article | Academy

Why Is It Necessary to Dry the SPE Sorbent Before Elution?

Proper drying of the SPE sorbent is a critical step to achieve high sensitivity, good reproducibility, and maximum analyte recovery, especially for complex sample matrices.
Phenomenex
tag
share
more
 Czech scientists develop a material for producing chemicals from water and sunlight, inspired by human enzymes
Article | Science and research

Czech scientists develop a material for producing chemicals from water and sunlight, inspired by human enzymes

Czech scientists from CATRIN and CEET developed an enzyme-inspired photocatalyst that produces hydrogen peroxide from sunlight, water and oxygen—without toxic solvents or precious metals.
CATRIN
tag
share
more
Oligos Made Easy - Part 3: IP-RP
Article | Science and research

Oligos Made Easy - Part 3: IP-RP

Discover how ion-pair reversed-phase (IP-RP) chromatography enables high-resolution oligonucleotide analysis. Learn about ion-pair selection, method setup, LC-MS compatibility, and robust separation strategies.
KNAUER
tag
share
more
News from LabRulezLCMS Library - Week 08, 2026
Article | Application

News from LabRulezLCMS Library - Week 08, 2026

This week we bring you application notes by Agilent Technologies, KNAUER, Shimadzu and Waters Corporation and presentation by MDCW / Clemson University!
LabRulez
tag
share
more
 

Related content

Overcoming Strong Solvent Effects in the Analysis of Vepdegestrant

Applications
| 2026 | Agilent Technologies
Instrumentation
HPLC
Manufacturer
Agilent Technologies
Industries
Pharma & Biopharma

Identification of Double Bond Positions and Relative Acyl Chain Positions in Egg Yolk Phosphatidylcholines Using OAD-TOF System

Applications
| 2026 | Shimadzu
Instrumentation
LC/MS, LC/MS/MS, LC/TOF, LC/HRMS
Manufacturer
Shimadzu
Industries
Food & Agriculture

High Molecular-Weight Polysaccharide Characterization by SEC-MALS Using GTxResolve™ 1000 and 2000 Å SEC Columns

Applications
| 2026 | Waters
Instrumentation
GPC/SEC, Consumables, LC columns
Manufacturer
Waters
Industries
Pharma & Biopharma, Food & Agriculture

Development and Optimization for a Comprehensive LC/MS/MS Method for the Detection of 74 PFAS Compounds

Applications
| 2026 | Agilent Technologies
Instrumentation
LC/MS, LC/MS/MS, LC/QQQ
Manufacturer
Agilent Technologies
Industries
Food & Agriculture

PFAS in Biota: Risk Context & Robust Analytical Solutions

Others
| 2026 | ALS Europe
Instrumentation
Laboratory analysis, LC/MS, LC/MS/MS
Manufacturer
Industries
Environmental
 

Related articles

Why Is It Necessary to Dry the SPE Sorbent Before Elution?
Article | Academy

Why Is It Necessary to Dry the SPE Sorbent Before Elution?

Proper drying of the SPE sorbent is a critical step to achieve high sensitivity, good reproducibility, and maximum analyte recovery, especially for complex sample matrices.
Phenomenex
tag
share
more
 Czech scientists develop a material for producing chemicals from water and sunlight, inspired by human enzymes
Article | Science and research

Czech scientists develop a material for producing chemicals from water and sunlight, inspired by human enzymes

Czech scientists from CATRIN and CEET developed an enzyme-inspired photocatalyst that produces hydrogen peroxide from sunlight, water and oxygen—without toxic solvents or precious metals.
CATRIN
tag
share
more
Oligos Made Easy - Part 3: IP-RP
Article | Science and research

Oligos Made Easy - Part 3: IP-RP

Discover how ion-pair reversed-phase (IP-RP) chromatography enables high-resolution oligonucleotide analysis. Learn about ion-pair selection, method setup, LC-MS compatibility, and robust separation strategies.
KNAUER
tag
share
more
News from LabRulezLCMS Library - Week 08, 2026
Article | Application

News from LabRulezLCMS Library - Week 08, 2026

This week we bring you application notes by Agilent Technologies, KNAUER, Shimadzu and Waters Corporation and presentation by MDCW / Clemson University!
LabRulez
tag
share
more
Other projects
GCMS
ICPMS
Follow us
FacebookX (Twitter)LinkedInYouTube
More information
WebinarsAbout usContact usTerms of use
LabRulez s.r.o. All rights reserved. Content available under a CC BY-SA 4.0 Attribution-ShareAlike