<p><strong>Symposium &quot;Advances in Chromatography and Electrophoresis &amp; Chiranal 2022&quot; belongs to the periodical meetings of specialists working in the field of separation methods.</strong></p>
<p>The biennial conference is organized by Department of Analytical Chemistry, Faculty of Science, <a href="https://lcms.cz/companies/17"><strong>Palacky University</strong></a> in cooperation with Regional Centre of Advanced Technologies and Materials, Czech Chemical Society and <a href="https://lcms.cz/companies/21"><strong>Czech Society for Mass Spectrometry</strong></a>. It is organized as a meeting of the scientists from the universities, academic institutes and experts from the routine analytical laboratories.</p>
<p>The scientific program will deal with all areas of separation science <strong>from fundamental to applied reseach</strong> in chemistry, physics, biology, medicine, forensic science and related disciplines. Official languages of the conference are <strong>English</strong>, <strong>Czech</strong> and <strong>Slovak</strong>.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UPOL_Katedra_analyticke_chemie_Pr_F_UP_Advances_in_Chromatography_and_Electrophoresis_and_Chiranal_2022_Programme_5817ff6a89/UPOL-Katedra-analyticke-chemie-PrF-UP-Advances-in-Chromatography-and-Electrophoresis-and-Chiranal-2022-Programme.webp" alt="UPOL: Katedra analytické chemie PřF UP - Advances in Chromatography and Electrophoresis &amp; Chiranal 2022 - Programme"/></p>
<h3><strong>Programme</strong></h3>
<h3><strong>Monday 13th June</strong></h3>
<h5><strong>14:00 – 17:30 Registration</strong></h5>
<ul>
<li>Fort Science, foyer, ground floor</li>
<li>Individual arrival of participants and accommodation</li>
</ul>
<h5><strong>16:00 – 16:15 Opening of conference</strong></h5>
<ul>
<li>Fort Science, Laudon Hall, ground floor</li>
<li>Presence of official representatives of Palacký University and Czech Chemical Society</li>
<li><strong>Foreword: Petr Bednář</strong></li>
</ul>
<h5><strong>16:15 – 17:15 Plenary Lecture</strong> (Chairmen: <a href="https://lcms.cz/articles?search=Karel+Lemr">Karel Lemr</a>, Juraj Ševčík)</h5>
<ul>
<li>Fort Science, Laudon Hall, ground floor</li>
</ul>
<h5><strong>Innovations in microchip electrophoresis: from design to applications. Honoring the memory of Prof. Milan Hutta</strong></h5>
<ul>
<li><strong>Marián Masár</strong></li>
</ul>
<h5><strong>17:30 – ?? Welcome party + banquet</strong></h5>
<ul>
<li>Fort Science, Laudon Hall, ground floor</li>
</ul>
<h3><strong>Tuesday 14th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
<li>Installation of posters (Faculty of Science, seminary rooms, 2nd floor)</li>
</ul>
<h5><strong>09:00 – 11:00 Lectures I: Electromigration methods and chip technologies</strong> (Chairmen: Michael Lämmerhofer, Petr Bednář)</h5>
<ul>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>09:00 – 09:30 Electrokinetic and microchip design for proteomics, interactomics and diagnostics</strong></h5>
<ul>
<li><strong>Anne Varenne</strong></li>
</ul>
<h5><strong>09:30 – 10:00 Non-invasive samples in clinical diagnostics: Can capillary electrophoresis be a suitable diagnostic tool?</strong></h5>
<ul>
<li><strong>Petr Kubáň</strong></li>
</ul>
<h5><strong>10:00 – 10:20 Chiral capillary electrophoresis with diquats as both passive and active players in the enantioseparation games</strong></h5>
<ul>
<li><strong>Václav Kašička</strong></li>
</ul>
<h5><strong>10:20 – 10:40 Simul 6 – A fast simulation software for electrophoresis</strong></h5>
<ul>
<li><strong>Bohuslav Gaš</strong></li>
</ul>
<h5><strong>10:40 – 11:00 Monolithic silicas in high performance liquid chromatography – unique material with lots of benefits</strong></h5>
<ul>
<li><strong>Egidijus Machtejevas</strong> (Merck)</li>
</ul>
<h5><strong>11:00 – 11:20 How we had fun during the lockdown, challenges that new products brought</strong></h5>
<ul>
<li><strong>Luděk Vlk</strong> (Chromservis)</li>
</ul>
<h5><strong>11:20 – 11:40 Coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>11:40 – 13:40 Lectures II: Multidimensional chromatography, mass spectrometry of small molecules</strong> (Chairmen: Petr Kubáň, Petr Barták)</h5>
<ul>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>11:40 – 12:10 Multidimensional liquid chromatography and pharmaceutical applications</strong></h5>
<ul>
<li><strong>Michael Lämmerhofer</strong></li>
</ul>
<h5><strong>12:10 – 12:40 Novel approaches to overcome dilution in comprehensive two-dimensional liquid chromatography</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Petr+%C4%8Cesla"><strong>Petr Česla</strong></a></li>
</ul>
<h5><strong>12:40 – 13:00 Application of flow-modulated comprehensive two-dimensional gas chromatography in chiral analysis of wine</strong></h5>
<ul>
<li><strong>Olga Vyviurska</strong></li>
</ul>
<h5><strong>13:00 – 13:20 Pharmaceutical analysis in the development of water-soluble prodrug of ICRF–193, a protective agent against anthracycline cardiotoxicity</strong></h5>
<ul>
<li><strong>Petra Štěrbová-Kovaříková</strong></li>
</ul>
<h5><strong>13:20 – 13:40 Achieving robust profiling, sensitive quantitation and confident unknown identification for small molecules applications with <a href="https://lcms.cz/products/403">Orbitrap Exploris 120 MS</a></strong></h5>
<ul>
<li><strong>Maciej Bromirski</strong> (<a href="https://lcms.cz/companies/42">Pragolab</a>)</li>
</ul>
<h5><strong>13:40 – 14:40 Lunch</strong></h5>
<ul>
<li>Mensa</li>
</ul>
<h5><strong>14:40 – 16:00 Poster session I (odd poster numbers) and coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, seminary rooms, 2nd floor</li>
</ul>
<h5><strong>16:00 – 17:50 Lectures III: Current trends in chromatography</strong> (Chairmen: Václav Kašička, Ondřej Kurka)</h5>
<ul>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>16:00 – 16:30 Comparison of lipid class and lipid species separation approaches in the lipidomic analysis</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Michal+Hol%C4%8Dapek"><strong>Michal Holčapek</strong></a></li>
</ul>
<h5><strong>16:30 – 17:00 Miniaturization as one of the trends in modern analytical chemistry</strong></h5>
<ul>
<li><strong>Zeineb Aturki</strong></li>
</ul>
<h5><strong>17:00 – 17:20 The possibilities of chiral separation of anionic boron clusters</strong></h5>
<ul>
<li><strong>Radim Kučera</strong></li>
</ul>
<h5><strong>17:20 – 17:40 Electron activated dissociation as potential solution to identification and quantitation of isomers using mass spectrometry without additional separation techniques</strong></h5>
<ul>
<li><strong>Tomáš Korba</strong> (Amedis)</li>
</ul>
<h5><strong>17:40 – 18:00 HPLC separation in metabolomic studies: challenges and solutions</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Ond%C5%99ej+Lacina"><strong>Ondřej Lacina</strong></a> (<a href="https://lcms.cz/companies/37">HPST</a>)</li>
</ul>
<h5><strong>18:00 – 19:00 Dinner</strong></h5>
<ul>
<li>Mensa</li>
</ul>
<h5><strong>19:30 – 21:00 Informal evening visit to the laboratories of the Department of Analytical Chemistry – including the Cyclic ion mobility MS lab</strong></h5>
<ul>
<li><a href="https://lcms.cz/products/202"><strong>SELECT SERIES Cyclic IMS instrument</strong></a>, third installed instrument with this unique technology worldwide</li>
</ul>
<h3><strong>Wednesday 15th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>09:00 – 11:20 Lectures IV:  Applications and practice of chromatography, heritage science, forensic science</strong> (Chairmen: Radim Kučera, Lukáš Kučera)</h5>
<ul>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>09:00 – 09:30 Art of chromatography – Chromatography in art</strong></h5>
<ul>
<li><strong>Václav Pitthard</strong></li>
</ul>
<h5><strong>09:30 – 10:20 Forensic applications of analytical chemistry</strong></h5>
<ul>
<li><strong>Luboš Valerián, Jan Aufart</strong></li>
</ul>
<h5><strong>10:20 – 10:40 Cyclic ion mobility – mass spectrometry of isomeric cathinones</strong></h5>
<ul>
<li><strong>Marianna Nytka</strong></li>
</ul>
<h5><strong>10:40 – 11:00 Intact protein analysis in biological fluids by liquid chromatography – triple quadrupole mass spectrometry</strong></h5>
<ul>
<li><strong>Katarína Maráková</strong></li>
</ul>
<h5><strong>11:00 – 11:20 Practical approaches for identifying equivalent and orthogonal columns</strong></h5>
<ul>
<li><strong>Heiko Behr</strong> (Phenomenex)</li>
</ul>
<h5><strong>11:20 – 13:00 Poster session II and coffee break</strong></h5>
<ul>
<li>Even poster numbers</li>
<li>Faculty of Science, foyer, seminary rooms, 2nd floor</li>
</ul>
<h5><strong>13:00 – 14:00 Lunch</strong></h5>
<ul>
<li>Mensa</li>
</ul>
<h5><strong>15:00 – 17:00 Social/sport event</strong></h5>
<ul>
<li>Bowling at Bowland Olomouc</li>
<li>A tour of Regional Office of National Heritage Institute (see details at the bottom of the page)</li>
</ul>
<h5><strong>18:00 – ?? Social dinner</strong></h5>
<ul>
<li>Faculty of Science, foyer, 6th floor</li>
</ul>
<h3><strong>Thursday 16th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>09:00 – 10:30 Lectures V: Supercritical fluid chromatography, pharmaceutical analysis</strong> (Chairmen: Dalibor Šatínský, Petr Fryčák)</h5>
<ul>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>09:00 – 09:30 Supercritical fluid chromatography mass spectrometry coupling: where are we?</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Lucie+Nov%C3%A1kov%C3%A1"><strong>Lucie Nováková</strong></a></li>
</ul>
<h5><strong>09:30 – 10:00 Reaction of basic analytes with carbon dioxide under supercritical fluid chromatography conditions: Confirmed</strong></h5>
<ul>
<li><strong>Michal Kohout</strong></li>
</ul>
<h5><strong>10:00 – 10:20 Unified chromatography – a step towards universal chromatographic methods</strong></h5>
<ul>
<li><strong>Volodymyr Pauk</strong></li>
</ul>
<h5><strong>10:20 – 10:40 Control of N-Nitrosamines in Pharmaceuticals</strong></h5>
<ul>
<li><strong>Jakub Jireš</strong></li>
</ul>
<h5><strong>10:40 – 11:00 Non-targeted UHPLC-DIA-HRMS approach for examination of impurities in levothyroxine formulations</strong></h5>
<ul>
<li><strong>Hana Chmelařová</strong></li>
</ul>
<h5><strong>11:00 – 11:20 Coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>11:20 – 13:30 Lectures VI: Novelty in sample preparation, ion mobility, data treatment</strong> (Chairmen: Lucie Nováková, Volodymyr Pauk)</h5>
<ul>
<li>Faculty of Science, assembly hall, 2nd floor)</li>
</ul>
<h5><strong>11:20 – 11:50 Advanced extraction approaches to sample extraction in flow and chromatography techniques based on nanofibers</strong></h5>
<ul>
<li><strong>Dalibor Šatínský</strong></li>
</ul>
<h5><strong>11:50 – 12:10 DisperSpin solid phase micro-extraction as a novel tool of phytohormonal analysis</strong></h5>
<ul>
<li><strong>Ivan Petřík</strong></li>
</ul>
<h5><strong>12:10 – 12:30 Monitoring of PAHs in simulated natural and artificial fires by HPLC-DAD-FLD with the application of Multi Component Integrated Calibration Method to improve quality of analytical results</strong></h5>
<ul>
<li><strong>Paweł Świt</strong></li>
</ul>
<h5><strong>12:30 – 12:50 Ion-mobility coupling with desorption electrospray ionization mass spectrometry imaging in plant isomer research</strong></h5>
<ul>
<li><strong>Chao Zhang</strong></li>
</ul>
<h5><strong>12:50 – 13:10 High resolution ion mobility separation</strong></h5>
<ul>
<li><strong>Alexandr Muck</strong> (<a href="https://lcms.cz/companies/50">Waters</a>)</li>
</ul>
<h5><strong>13:10 – 13:30 Using trapped ion mobility spectrometry to improve confidence in identification of knowns and unknowns</strong></h5>
<ul>
<li><strong>Noud van der Borg</strong> (Bruker)</li>
</ul>
<h5><strong>13:30 – 14:10 Closing of conference and farewell drink</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>14:10 – 15:10 Lunch</strong></h5>
<ul>
<li>Mensa</li>
</ul>
<h4><strong>Social programme:</strong></h4>
<h5><strong>A tour of Regional Office of National Heritage Institute</strong></h5>
<p>The Archaeological Department of the National Heritage Institute, Regional Office in Olomouc has been carrying out excavations in the territory of the former capital of Moravia for 50 years. In recent years, interdisciplinary cooperation with natural scientists – archaeobotanists, palynologists, anthropologists and, last but not least, chemists from the Faculty of Science of Palacký University – has been successfully developing. Examples of jointly researched artefacts include a prehistoric depot from the site of Benkov near Uničov, medieval silk textiles from Křížkovského Street in Olomouc or small objects of daily devotion in the modern period from the same research.</p>
<h5><strong>Sporting event:</strong></h5>
<p>Bowling in BOWLAND Bowling Center, Šantovka</p>


Advances in Chromatography and Electrophoresis & Chiranal 2022 - Programme

<p><strong>STARSS project is approaching its end after more than five years. A few more months remain before it will be concluded. The major objective of the project was to create a strong team of researchers enabling significant progress in the field of the separation science. The annual STARSS conferences have always been a good opportunity to bring people from several Czech and European institutions in Hradec Kralove where we have met face to face for a few days in order to inform each other about the progress in the research. It is clear that the focus of these conferences has been on the exchange in scientific information. However, an integral part of our previous conferences has also included informal meetings when we had time to talk about issues sometimes even completely outside of science perhaps with a drink and food. Social part of conferences is simply indispensable.</strong></p>
<p><strong>I trust that this conference will be at least as interesting as all the previous ones. Its program is rich and includes presentations by renowned “icons” of separation science from both abroad and the Czech Republic, as well as talks given by students and postdocs. Such “cross-pollination” will certainly be uplifting for all involved. I wish all those who attend this conference a pleasant stay in Hradec Králové and enjoyable conferencing.</strong></p>
<p><strong><a href="https://lcms.cz/articles?search=Franti%C5%A1ek+%C5%A0vec">Prof. František Švec</a></strong></p>
<h3><strong>Program: 6th STARSS Conference on Separation Science</strong></h3>
<ul>
<li><a href="https://portal.faf.cuni.cz/getattachment/Projects/STARSS/Events/6th-STARSS-conference/Program/2022-STARSS-booklet.pdf.aspx?lang=en-GB"><strong>Conference booklet including speakers bio</strong></a></li>
</ul>
<h3><strong>DAY 1: Monday 17. 10. 2022</strong></h3>
<h5><strong>08:00 – 09:00 REGISTRATION</strong></h5>
<h5><strong>09:00 – 09:20 Welcoming address</strong></h5>
<ul>
<li>Prof. Petr Solich, Prof. František Švec, Prof. Lucie Nováková</li>
<li>Assoc. Prof. Jaroslav Roh (the dean of the Faculty of Pharmacy, Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h4><strong>SESSION I: IMPORTANCE OF SEPARATION SCIENCE FOR CLINICAL APPLICATIONS</strong></h4>
<ul>
<li>Chairperson: Prof. František Švec</li>
</ul>
<h5><strong>09:20 – 10:00 L-01: UHPSFC/MS as a powerful tool for high-throughput lipidomic quantitation in cancer screening</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Michal+Hol%C4%8Dapek">Prof. Michal Holčapek</a> (University of Pardubice, Czech Republic)</li>
</ul>
<h5><strong>10:00 – 10:40 L-02: Selected ion flow tube mass spectrometry, SIFT-MS, analyses of volatile metabolites in exhaled breath</strong></h5>
<ul>
<li>Prof. Patrik Španěl (J. Heyrovský Institute of Physical Chemistry, Prague, Czech Republic)</li>
</ul>
<h5><strong>10:40 – 11:10 Coffee-break and discussion</strong></h5>
<h4><strong>SESSION II: BIOPHARMACEUTICALS</strong></h4>
<ul>
<li>Chairperson: Prof. Lucie Nováková</li>
</ul>
<h5><strong>11:10 – 11:50 L-03: Simplifying the characterization of complex biopharmaceutical products through the use of ultra-short columns or multi-isocratic elution mode</strong></h5>
<ul>
<li>Dr. Davy Guillarme (University of Geneva, Switzerland)</li>
</ul>
<h5><strong>11:50 – 12:20 L-04: Analysis of therapeutic proteins by multidimensional LC: How far can we go?</strong></h5>
<ul>
<li>Dr. Valentina D ́Atri (University of Geneva, Switzerland)</li>
</ul>
<h5><strong>12:20 – 12:40 L-05: pyroLC: online thermal decomposition of proteins as a tool for ultra-fast bottom-up proteomics</strong></h5>
<ul>
<li>Dr. Juraj Lenčo (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>12:40 – 13:00 L-06: RPLC-UV and HILIC-UV characterization of home-made ramucirumab-based antibody-drug conjugate</strong></h5>
<ul>
<li>Denis Naplekov (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>13:00 – 14:00 Lunch</strong></h5>
<h4><strong>SESSION III: 2D SEPARATIONS</strong></h4>
<ul>
<li>Chairperson: Assoc. Prof. Jiří Urban</li>
</ul>
<h5><strong>14:00 – 14:40 L-07: What can we expect from LC x LC compared to conventional 1D-LC</strong></h5>
<ul>
<li>Prof. Sabine Heinisch (Université Claude Bernard Lyon 1, Lyon, France)</li>
</ul>
<h5><strong>14:40 – 15:20 L-08: Modelling of analyte profiles and band broadening generated by interface loops used in multi-dimensional liquid chromatography</strong></h5>
<ul>
<li>Prof. Ken Broeckhoven (Vrije Universiteit Brussel, Belgium)</li>
</ul>
<h5><strong>15:20 – 15:50 L-09: Off-line LC x SFC in the prospect of upgrading natural or recycled products</strong></h5>
<ul>
<li>Dr. Karine Faure (Université Claude Bernard Lyon 1, Lyon, France)</li>
</ul>
<h5><strong>15:50 – 16:20 L-10: Two-dimensional liquid chromatographic separations of complex samples of industrial products and intermediates</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Petr+%C4%8Cesla"><strong>Assoc. Prof. Petr Česla</strong></a> (University of Pardubice, Czech Republic)</li>
</ul>
<h5><strong>16:20 – 16:50 Coffee-break and discussion</strong></h5>
<h4><strong>SESSION IV: IMPORTANCE OF STEROIDS</strong></h4>
<ul>
<li>Chairperson: Prof. Marcela Segundo</li>
</ul>
<h5><strong>16:50 – 17:30 L-11: An image of the future of steroid biochemistry</strong></h5>
<ul>
<li>Prof. Ruth Andrew (University of Edinburgh, UK)</li>
</ul>
<h5><strong>17:30 – 18:00 L-12: Ecdysterone metabolism: A holistic view on compound identification for doping control</strong></h5>
<ul>
<li>Prof. Maria Kristina Parr (Freie Universität Berlin, Berlin, Germany)</li>
</ul>
<h5><strong>18:00 – 18:30 L-13: New liquid chromatography-tandem mass spectrometry method for targeted profiling of steroids in mouse plasma</strong></h5>
<ul>
<li>Taťána Gazárková (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>18:30 – 19:30 Welcome drink</strong></h5>
<h3><strong>DAY 2: Tuesday 18. 10. 2022</strong></h3>
<h5><strong>08:00 – 09:00 REGISTRATION</strong></h5>
<h4><strong>SESSION V: SUPERCRITICAL FLUID CHROMATOGRAPHY AND EXTRACTIONS</strong></h4>
<ul>
<li>Chairperson: Assoc. Prof. Radim Kučera</li>
</ul>
<h5><strong>09:00 – 09:30 L-14: Supercritical fluid extraction and chromatography in life science applications</strong></h5>
<ul>
<li>Prof. Charlotta Turner (Lund University, Lund, Sweden)</li>
</ul>
<h5><strong>09:30 – 10:00 L-15: Supercritical fluid chromatography – tandem mass spectrometry for analysis of vitamin D metabolites in biological samples</strong></h5>
<ul>
<li>Dr. Margareta Sandahl (Lund University, Lund, Sweden)</li>
</ul>
<h5><strong>10:00 – 10:30 L-16: The effect of make-up solvent composition on responses in SFC-MS: Electrospray vs UniSpray</strong></h5>
<ul>
<li>Dr. Kateřina Plachká (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>10:30 – 11:00 L-17: UHPSFC-MS/MS versus 2D-UHPLC-MS/MS in chiral separation of Propranolol and its Hydroxy Metabolites</strong></h5>
<ul>
<li>Lukas Harps (Freie Universität Berlin, Berlin, Germany)</li>
</ul>
<h5><strong>11:00 – 11:30 Coffee-break and discussion</strong></h5>
<h4><strong>SESSION VI: COLUMN TECHNOLOGY</strong></h4>
<ul>
<li>Chairperson: Prof. Dalibor Šatínský</li>
</ul>
<h5><strong>11:30 – 12:10 L-18: Hypercrosslinked monolithic stationary phases in proteomics</strong></h5>
<ul>
<li>Assoc. Prof. Jiří Urban (Masaryk University, Brno, Czech Republic)</li>
</ul>
<h5><strong>12:10 – 12:40 L-19: Guidelines for tuning the macropore structure of monolithic columns for high-performance liquid chromatography</strong></h5>
<ul>
<li>Dr. Sebastiaan Eeltink (Vrije Universiteit Brussel, Belgium)</li>
</ul>
<h5><strong>12:40 – 13:10 L-20: Strategies and chemistry in column development</strong></h5>
<ul>
<li>Assoc. Prof. Michal Kohout (University of Chemistry and Technology, Prague, Czech Republic)</li>
</ul>
<h5><strong>13:10 – 14:30 Lunch</strong></h5>
<h4><strong>SESSION VII: RETENTION MECHANISMS AND STATIONARY PHASES</strong></h4>
<ul>
<li>Chairperson: Prof. Spas Kolev</li>
</ul>
<h5><strong>14:30 – 15:00 L-21: Understanding the mechanism, advantages and limitations of hydrophilic interaction liquid chromatography</strong></h5>
<ul>
<li>Prof. David McCalley (University of West England, UK)</li>
</ul>
<h5><strong>15:00 – 15:30 L-22: Study of molecular structures through retention mechanisms in liquid chromatography: New trends and applications</strong></h5>
<ul>
<li>Dr. Jean-Christophe Garrigues (Paul Sabatier University - Toulouse III, Toulouse, France)</li>
</ul>
<h5><strong>15:30 – 16:00 L-23: Problems of stationary phases in SFC</strong></h5>
<ul>
<li>Prof. Lucie Nováková (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>16:00 – 16:30 L-24: The chromatographic behaviour of pharmaceuticals on stationary phases based on metal oxides</strong></h5>
<ul>
<li>Assoc. Prof. Radim Kučera (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>16:30 – 17:00 Coffee-break and discussion</strong></h5>
<h4><strong>SESSION VIII: SAMPLE PREPARATION AND APPLICATIONS</strong></h4>
<ul>
<li>Chairperson: Assoc. Prof. Hana Sklenářová</li>
</ul>
<h5><strong>17:00 – 17:30 L-25: Glucosinolate profiling in Brassicaceae family: instrumental analysis and biological application</strong></h5>
<ul>
<li>Dr. Iva Pavlovic (Palacký University, Olomouc, Czech Republic)</li>
</ul>
<h5><strong>17:30 – 18:00 L-26: Flow programming for the development of online solid-phase extraction</strong></h5>
<ul>
<li>Assoc. Prof. Petr Chocholouš (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>18:00 – 18:30 L-27: Automated tandem homogeneous liquid-liquid extraction and centrifugation-less deproteination for complex sample analysis on HPLC</strong></h5>
<ul>
<li>Dr. Burkhard Horstkotte (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>19:00 – 22:00 Conference dinner</strong></h5>
<h3><strong>DAY 3: Wednesday 19. 10. 2022</strong></h3>
<h4><strong>WORKSHOPS: FACULTY OF PHARMACY</strong></h4>
<h5><strong>08:00 – 10:30 W1: Supercritical fluid extraction of polar and nonpolar compounds from Eucalyptus sp.</strong></h5>
<ul>
<li>Laboratory 916, 8th floor, Faculty of Pharmacy</li>
<li>Dr. Veronika Pilařová (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>08:00 – 10:30 W2: MetAmino® Kit – simple and easy sample preparation method for your LC-MC or GC-MS analysis</strong></h5>
<ul>
<li>Student’s Laboratory, 3rd floor, Faculty of Pharmacy</li>
<li>Ing. Jana Volková (Chromservis, Czech Republic)</li>
</ul>
<h5><strong>10:30 – 11:00 Coffee-break and discussion</strong></h5>
<h5><strong>11:00 – 13:30 W3: Who will help you with sample prep and liquid handling?</strong></h5>
<ul>
<li>Student’s Laboratory, 4th floor, Faculty of Pharmacy</li>
<li>Dr. Martina Riesová (<a href="https://lcms.cz/companies/50"><strong>Waters</strong></a>, Prague, Czech Republic)</li>
</ul>
<h5><strong>13:30 – 14:30 Lunch</strong></h5>
<h5><strong>14:30 – 17:00 W4: Practical application of nanofibers for advanced extraction approaches in chromatography</strong></h5>
<ul>
<li>Student’s Laboratory no. 524, 4th floor, Faculty of Pharmacy</li>
<li>Prof. Dalibor Šatínský, Dr. Ivona Lhotská (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>17:00 – 18:00 Coffee-break and discussion</strong></h5>


6th STARSS Conference on Separation Science (Program)

<p><strong>Prof. Ing. Michal Kohout, Ph.D. is originally from southern Bohemia. He started studying at UCT Prague in 2000 after high school in Soběslav. After his postdoctoral research at the Institut of Analytical Chemistry of the University of Vienna, he decided to return to his alma mater, UCT Prague, and extend, apply and transfer knowledge and experience he gained abroad. He was recently named full professor and, in addition to research and teaching, considers his mission as professor to be setting an example for students in terms of not being afraid to start one’s own business since he, with colleagues, founded a UCT Prague spin-off company, Galochrom, discussed below.</strong></p>
<h5><strong>What does your research group do? What is the focus of your research activities?</strong></h5>
<p>We have been dealing with liquid crystals for a very long time. Liquid crystals are organic materials with various applications, and as research evolves, it’s expected that liquid crystals will have some added value in real-life applications, beyond simply being liquid crystalline (e.g. being chiral, sensitive to light or to magnetic fields). We try to modify molecules so that they can handle the various demands that modern materials chemistry places on them.</p>
<p>The second area of focus, chiral stationary phases for liquid chromatography, I brought back with me to UCT Prague from my post-doc stay. In liquid chromatography, we study systems that are used in the analysis of drugs (if an active substance is chemically and optically pure), for example.</p>
<p>Together with my colleagues and students, we also work on various side projects in classical organic chemistry, such as organocatalysis.</p>
<h5><strong>Most people can only conceptualize solid crystals. Can you introduce us to the world of liquid crystals?</strong></h5>
<p>It’s quite simple: liquid crystals are basically crystals too. One can imagine a crystal as being an ordered and oriented structure with molecules precisely and unambiguously arranged in space. Liquid crystals also have these characteristics, but their molecules can also move and are mobile even though they have, let’s say, a partially-preserved crystalline structure, for they are in the so-called mesophase. To conceptualize this, one could simply compare this to a system of straws floating in the same direction, because the molecules are arranged like this and are able to move when acted upon by an external impulse (e.g. electric or magnetic field or light). A structure can be rearranged so that it occupies a new, lowest energy arrangement. Liquid crystals are used, for example, in optoelectric devices, and we’re all surrounded by liquid crystals, which are not just used in monitors, televisions, and watch displays, but also in soap or cell membranes. One doesn’t even realize how many liquid crystalline substances and systems actually are all around us in the real world.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/VSCHT_Pokousime_se_uspet_se_spin_off_firmou_Galochrom_profesor_Michal_Kohout_e7be735e35.jpg" alt="UCT Prague: We are trying to succeed with the spin-off company Galochrom"/></p>
<h5><strong>Is there anything else you’re up to that you’d like to tell us about?</strong></h5>
<p>I’d put liquid chromatography into the background a bit. Let me put it this way: the world of liquid crystals research is a kind of long-distance race, and we don’t know for sure if and when relatively complex molecules will be used. They certainly have the potential to be used in real life, but in the distant future. It is not easy to prepare and enhance them, so that they meet various requirements and do as many extra things as we would like.</p>
<p>On the other hand, if we look at the area of chromatography, the real world application of new research results happens relatively quickly. If we prepare a chromatography column that is significantly better than what’s currently on the market, it might be commercially interesting and result in a product that we’ll see on the market in our lifetimes.</p>
<p>But it bothers me and my colleagues that even the development of unique stationary phases is still too slow, that it takes too long for a chromatographic column to reach the market. In 2017, we tried to approach several large manufacturers with a unique system we patented. Unfortunately, there was no interest in this technology because manufactures would have to change their production technologies slightly. Because of this, my colleagues and I decided to found our own company, the Galochrom, UCT Prague spin-off. This way, we also wish to show younger colleagues and students that they don’t have to be afraid of starting a company and entering the business world.</p>
<h5><strong>How is Galochrom doing?</strong></h5>
<p>We are in the early phases. We founded the spin-off in September 2023, and since then, we placed second in a European start-up competition, which we consider to be a great success. We are currently trying to put the first products on the market and prove that they are better than those already available. Of course, we believe that our products will be adopted.</p>
<h5><strong>What practical value do you think your research and professional activities have for society and your field?</strong></h5>
<p>Our multimodal columns will have a wider range of applications and will thus be able to replace three to four standard columns. This will lead to dramatic savings for end users as well as lower solvent and solvent disposal costs.</p>
<p>As for liquid crystals, they’re a long shot, as I’ve already said, but we might, for example, use a system of so-called dopants. Let’s take a photosensitive material. We don’t have to use it as a single component; instead, we can add it to a cheaper commercial material and mix it (5-10%). Thanks to the dopant, an entire system is sensitive to light and we can not only switch individual parts (pixels) from the mesophase to the liquid phase, but also read this information with a laser. Such systems could serve as optical memories.</p>
<h5><strong>You recently became a full professor. What does this mean for you? What do you teach and what are your first impressions as professor?</strong></h5>
<p>Of course, I consider this a great personal success built upon the great work and support by my students, colleagues, and mentors. My original motivation for entering academia, a long time ago, was a promise I made to my dad: that I would graduate as one of the best in my class at UCT Prague. And I’ve made it this far, so I think he would be proud of me and that fills me with a certain sense of fulfilment. I teach organic chemistry. So for first impressions: I have more work, but it’s interesting for me, nevertheless, to teach students who are just starting their university programs. My lectures before this were for advanced students and doctoral candidates. All of a sudden, when you’re teaching core subjects, you start trying not to influence students’ mind sets too much while still conveying the information in some absorbable way, reassuring them that chemistry is actually cool and that chemists are pretty normal people.</p>
<h5><strong>It must be quite challenging to capture the attention of an auditorium full of people and to decide how much to go into detail. Do you struggle with this kind of thing? Do you have any tricks for engaging your audience?</strong></h5>
<p>It’s true, when you enter an auditorium and there are 180 people sitting there instead of 20, it’s really an incredible difference. It’s not so much about the number of people itself, because there are about the same number of people at conference presentations, but conference attendees are experts who aren’t really surprised by anything in the field. Teaching core subjects is rather about setting some level of comprehensibility for what you’re explaining.</p>
<p>Gradually, I’m getting faster at preparing my lectures, but at first, it was very time-consuming. Yet, during the class, sometimes I still get taken away a bit and then I get behind and play catch up. (He laughs.)</p>
<p>As for student engagement, I tell my colleagues that I’m trying to use my latent theatre talents, so hope that&#39;s at least somewhat successful. I’m going back in time a couple of decades and am going to use some simple, themed experiments from back then in my lectures.</p>
<h5><strong>You started studying at UCT Prague in 2000. What do you think has changed the most for students since then?</strong></h5>
<p>Definitely the quality of the food in the canteens. One hundred percent! But what has really changed for the better are the travel possibilities. That&#39;s the biggest shift I see. Today, it’s easy to go abroad for a semester, almost whenever you want. There are countless ways to do this. I remember how I tried to do an internship during my doctoral studies, but preparations were crazy. So instead, I arranged at a conference with my mentor’s colleague to join her laboratory within a European project because it was so much easier to go this way. Going abroad gives you a wealth of new experiences, and it’s great that this option is much more accessible than before.</p>
<h5><strong>Is there anything that you did not like as a student and managed to change—or are at least trying to change—as a professor?</strong></h5>
<p>For a relatively long time, my colleagues and I tried to appeal to UCT Prague’s management to provide competitive scholarships for doctoral students. Doctoral candidates are the UCT Prague’s main strength because without them, the university couldn’t do basic research. They should be valued accordingly. So I’m a supporter of finding ways to increase scholarships and I think we’ve been quite successful in this regard.</p>
<h5><strong>What would you like to achieve in your professional and personal lives?</strong></h5>
<p>As a professional, I would like that the things we’ve invented would actually be used. Research, whatever form, should aim at developing things that are useful for humanity. Basic research is great, but I like finding applications while striving to advance both science and society as a whole. Why else would we be here?</p>
<p>Personally, my highest priority is work-life balance. Achieving some reasonable balance with work, what I like to do, and time for family.</p>
<h5><strong>What does the future hold for your research group?</strong></h5>
<p>The long-term plan involves spin-off companies and connecting academia with the commercial sector. Engagement in basic research is necessary for finding usable materials and applications, yet connecting these applications directly to the industrial sector is crucial for us. The plan is to try to make a platform for quick and simple technology transfer at UCT Prague.</p>
<h5><strong>What do you do when you turn out the lights in your lab?</strong></h5>
<p>I have hobbies I’ve done for a long time. I’m either going to volleyball or trying to go fishing. Fishing is more time-consuming, so I mainly stick to volleyball. And, of course, I’m running home to spend time with my loved ones.</p>


We are trying to succeed with the spin-off company Galochrom

<p><strong>The biennial conference is organized by Department of Analytical Chemistry, Faculty of Science, Palacky University in cooperation with Czech Chemical Society and Czech Society for Mass Spectrometry. It is organized as a meeting of the scientists from the universities, academic institutes and experts from the routine analytical laboratories.</strong></p>
<p>The scientific program will deal with all areas of separation science from fundamental to applied reseach in chemistry, physics, biology, medicine, forensic science and related disciplines. Official languages of the conference are English, Czech and Slovak.</p>
<h3><strong>Registration</strong></h3>
<blockquote>
<p><a href="https://chiranal2024.upol.cz/index.php?link=registration"><strong>Registration</strong></a></p>
</blockquote>
<p>Registration fee is 5000,- CZK (200 EUR) + tax 21 %.</p>
<p>Registration covers printed Program and Book of Abstracts, access to all scientific and social events held in the framework of the conference and meals.</p>
<p>The invoice should be paid until <strong>May 26th, 2024</strong>.</p>
<h3><strong>Programme</strong></h3>
<h3><strong>Monday 17th June</strong></h3>
<h5><strong>14:00 – 17:30 Registration</strong></h5>
<ul>
<li>Fort Science (foyer, ground floor), individual arrival of participants and accommodation</li>
</ul>
<h5><strong>16:00 – 16:15 Opening of the conference (Fort Science, Laudon Hall, ground floor), presence of official representatives of the conference partners</strong></h5>
<ul>
<li>Foreword: Petr Bednář</li>
</ul>
<h5><strong>16:15 – 17:15 Plenary Lecture</strong></h5>
<ul>
<li>Fort Science, Laudon Hall, ground floor</li>
</ul>
<h5><strong>Toward unrivaled chromatographic resolving power: Design and development of comprehensive spatial three-dimensional liquid-phase separation technology</strong></h5>
<ul>
<li>Sebastiaan Eeltink</li>
</ul>
<h5><strong>17:30 – 17:45 Ceremonial launch of the book of Lucie Nováková, Michal Douša, Petr Česla and Jiří Urban: Modern HPLC separations in theory and practice</strong></h5>
<h5><strong>17:45 – ?? Welcome party + banquet</strong></h5>
<ul>
<li>Fort Science, Laudon Hall, ground floor</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/UPOL_Chiranal_cover_image_6d8ebc4308/UPOL-Chiranal-cover-image.jpg" alt="Chiranal: Advances in Chromatography and Electrophoresis &amp; Chiranal 2024"/></p>
<h3><strong>Tuesday 18th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
<li>installation of posters (Faculty of Science, seminary rooms, 2nd floor)</li>
</ul>
<h4><strong>09:00 – 11:20 Lectures I: Electromigration techniques, ion exchange chromatography</strong></h4>
<ul>
<li>chairmen: Michael Lämmerhofer, Petr Bednář</li>
<li>Faculty of Science, assembly hall, 2nd floor)</li>
</ul>
<h5><strong>09:00 – 09:30 Electrokinetic methodologies for diagnostics development</strong></h5>
<ul>
<li>Anne Varenne</li>
</ul>
<h5><strong>09:30 – 10:00 Advances in microchip electrophoresis for the analysis of environmental and biological samples</strong></h5>
<ul>
<li>Marián Masár</li>
</ul>
<h5><strong>10:00 – 10:20 Determination of effective charge and ionic mobilities of highly sulfated cyclodextrins by capillary isotachophoresis and zone electrophoresis</strong></h5>
<ul>
<li>Václav Kašička</li>
</ul>
<h5><strong>10:20 – 10:40 Capillary Zone Electrophoresis – Mass spectrometry for intact protein analysis in biological fluids – possibilities and limitations</strong></h5>
<ul>
<li>Katarína Maráková</li>
</ul>
<h5><strong>10:40 – 11:00 Microcontrollers and their application in constructing analytical instrumentation</strong></h5>
<ul>
<li>Petr Kubáň</li>
</ul>
<h5><strong>11:00 – 11:20 Adsorbable organic fluorine (AOF) – a sum parameter for non-targeted screening of per- and polyfluorinated alkyl substances (PFASs) in waters</strong></h5>
<ul>
<li>Jan Soukup (<a href="https://lcms.cz/companies/176"><strong>Metrohm</strong></a>)</li>
</ul>
<h5><strong>11:20 – 11:40 Coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h4><strong>11:40 – 13:40 Lectures II: Approaches to method development in LC and MS, capillary chromatography</strong></h4>
<ul>
<li>chairmen: Anne Varenne, Petr Barták</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>11:40 – 12:10 How to successfully analyse oligonucleotides with liquid chromatography</strong></h5>
<ul>
<li>Davy Guillarme</li>
</ul>
<h5><strong>12:10 – 12:40 Recent applications of nano-liquid chromatography for the characterization of plant and food products</strong></h5>
<ul>
<li>Zeineb Aturki</li>
</ul>
<h5><strong>12:40 – 13:00 Personal insights on the design of experiments</strong></h5>
<ul>
<li>Jiří Urban</li>
</ul>
<h5><strong>13:00 – 13:20 How to make chromatography more sustainable</strong></h5>
<ul>
<li>Egidijus Machtejevas (Merck)</li>
</ul>
<h5><strong>13:20 – 13:40 Automated workflow for derivatized analyte analysis in LC-TIMS-MS/MS 4D-metabolomics data: Incorporating in-silico</strong></h5>
<ul>
<li>Aiko Barsch (Bruker Daltonics)</li>
</ul>
<h5><strong>13:40 – 14:40 Lunch</strong></h5>
<ul>
<li>university cafeteria</li>
</ul>
<h5><strong>14:40 – 16:00 Poster session I (odd poster numbers) and coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, seminary rooms, 2nd floor</li>
</ul>
<h4><strong>16:00 – 18:00 Lectures III: Current trends in chromatography</strong></h4>
<ul>
<li>chairmen: Václav Kašička, Ondřej Kurka</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>16:00 – 16:30 Isomer separations in metabolomics and lipidomics</strong></h5>
<ul>
<li>Michael Lämmerhofer</li>
</ul>
<h5><strong>16:30 – 17:00 New Trends in the Lipidomic and Metabolomic Analysis</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Michal+Hol%C4%8Dapek"><strong>Michal Holčapek</strong></a></li>
</ul>
<h5><strong>17:00 – 17:20 Unveiling the Power of Deep UV LAESI/LAESCI Dual Ambient Ionization: Towards Comprehensive Molecular MSI</strong></h5>
<ul>
<li>Barbora Papoušková</li>
</ul>
<h5><strong>17:20 – 17:40 Advanced small molecule mass spectrometry</strong></h5>
<ul>
<li>Luka Milivojevic (<a href="https://lcms.cz/companies/42"><strong>Pragolab</strong></a>/Thermo Fisher Scientific)</li>
</ul>
<h5><strong>17:40 – 18:00 Employing ballistic gradients, vacuum jacketed columns and prototype benchtop multi reflecting time-of-flight (MRT) to increase lipidomic analytical throughput whilst maintainig highly confident identifications</strong></h5>
<ul>
<li>Alex Muck (<a href="https://lcms.cz/companies/50"><strong>Waters</strong></a>)</li>
</ul>
<h5><strong>18:00 – 19:00 Dinner</strong></h5>
<ul>
<li>university cafeteria</li>
</ul>
<h5><strong>19:15 – 21:00 Excursion to the Eureka University Brewery and Department of Analytical Chemistry</strong></h5>
<h3><strong>Wednesday 19th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h4><strong>09:00 – 11:20 Lectures IV: Advances in biomolecules analysis, heritage science</strong></h4>
<ul>
<li>chairmen: Sebastiaan Eeltink, Lukáš Kučera</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>09:00 – 09:30 Advances in chiral separation of carboranes – new phenyl ring isosteres for drug development</strong></h5>
<ul>
<li>Radim Kučera</li>
</ul>
<h5><strong>09:30 – 10:00 Analysis of selected drugs in whole blood collected with a volumetric absorptive microsampling device</strong></h5>
<ul>
<li>Petra Štěrbová-Kovaříková</li>
</ul>
<h5><strong>10:00 – 10:20 Translation of omics into clinics</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=David+Friedeck%C3%BD"><strong>David Friedecký</strong></a></li>
</ul>
<h5><strong>10:20 – 10:40 Py/GC-MS characterization of oil paintings to correlate museum environment and materials condition over time for SMARTMUS-e project</strong></h5>
<ul>
<li>Paola Lucero</li>
</ul>
<h5><strong>10:40 – 11:00 Recent chromatographic advances in plant hormone profiling methods</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Ond%C5%99ej+Nov%C3%A1k"><strong>Ondřej Novák</strong></a> (on behalf of <a href="https://lcms.cz/companies/37"><strong>Altium</strong></a>)</li>
</ul>
<h5><strong>11:20 – 13:00 Poster session II (even poster numbers) and coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, seminary rooms, 2nd floor</li>
</ul>
<h5><strong>13:00 – 14:00 Lunch</strong></h5>
<ul>
<li>university cafeteria</li>
</ul>
<h5><strong>15:00 – 17:00 Social/sport event</strong></h5>
<ul>
<li>Bowling at Bowland Olomouc</li>
<li>A tour around Olomouc, visit of Regional Museum in Olomouc</li>
</ul>
<h5><strong>18:00 – ?? Social dinner</strong></h5>
<ul>
<li>Faculty of Science, foyer, 6th floor</li>
</ul>
<h3><strong>Thursday 20th June</strong></h3>
<h5><strong>08:30 – 09:00 Breakfast</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>09:00 – 11:00 Lectures V: Matrix effects, chiral separation, advances in stationary phases, supercritical fluid chromatography</strong></h5>
<ul>
<li>chairmen: Zeineb Aturki, Petr Fryčák</li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>09:00 – 09:30 Matrix effects in LC-MS: importance of correct calculation approach</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Lucie+Nov%C3%A1kov%C3%A1"><strong>Lucie Nováková</strong></a></li>
</ul>
<h5><strong>09:30 – 10:00 New trends in ion exchange-type chiral stationary phases and their chiral recognition mechanisms</strong></h5>
<ul>
<li><a href="https://lcms.cz/article/3991"><strong>Michal Kohout</strong></a></li>
</ul>
<h5><strong>10:00 – 10:20 Development of an easy-to-set-up multiple heart-cutting achiral-chiral LC-LC method for the analysis of branched-chain amino acids in food supplements</strong></h5>
<ul>
<li>Ghaid W.A. Abualzulof</li>
</ul>
<h5><strong>10:20 – 10:40 Rapid profiling of bioactive compounds using supercritical fluid chromatography coupled with tandem mass spectrometry</strong></h5>
<ul>
<li>Ivan Petřík</li>
</ul>
<h5><strong>10:40 – 11:00 Symbiosis of core-shell and fully porous particles: Implementing both for better HPLC and UHPLC results</strong></h5>
<ul>
<li>Jan Vlasák (<a href="https://lcms.cz/companies/51"><strong>Phenomenex</strong></a>)</li>
</ul>
<h5><strong>11:00 – 11:20 Coffee break</strong></h5>
<ul>
<li>Faculty of Science, foyer, 2nd floor</li>
</ul>
<h5><strong>11:20 – 13:30 Lectures VI: Selectivity in chromatography and mass spectrometry and its optimization, analysis of natural products</strong></h5>
<ul>
<li>chairmen: Lucie Nováková, <a href="https://lcms.cz/articles?search=Tom%C3%A1%C5%A1+Pluh%C3%A1%C4%8Dek"><strong>Tomáš Pluháček</strong></a></li>
<li>Faculty of Science, assembly hall, 2nd floor</li>
</ul>
<h5><strong>11:20 – 11:50 Targeted and untargeted analysis of psilocybin mushrooms using LC-MS</strong></h5>
<ul>
<li>Kevin Schug</li>
</ul>
<h5><strong>11:50 – 12:10 On the potential of two-dimensional liquid chromatography for environmental analysis</strong></h5>
<ul>
<li>Deirdre Cabooter</li>
</ul>
<h5><strong>12:10 – 12:30 Leveraging ion mobility spectrometry-mass spectrometry data using machine learning reveals provenance of indigo dyes</strong></h5>
<ul>
<li>Volodymyr Pauk</li>
</ul>
<h5><strong>12:30 – 12:50 Numerical optimization of gradient separations in RP and HILIC using non-linear retention models</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Petr+%C4%8Cesla"><strong>Petr Česla</strong></a></li>
</ul>
<h5><strong>12:50 – 13:10 Do we need separations in clinical diagnostics?</strong></h5>
<ul>
<li>Rutuja Patil</li>
</ul>
<h5><strong>13:10 – 13:30 Metabolomics analysis of Moringa oleifera leaves: correlation between in vitro effect on C2C12 myotubes cell line and geographical origin</strong></h5>
<ul>
<li>Mariateresa Maldini (Amedis)</li>
</ul>
<h5><strong>13:30 – 14:10 Closing of the conference and farewell drink (Faculty of Science, foyer, 2nd floor)</strong></h5>
<h5><strong>14:10 – 15:10 Lunch (university cafeteria)</strong></h5>
<h3><strong>Partners and Sponsors</strong></h3>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Chiranal_2024_Partners_e3fc1710b0/Chiranal-2024-Partners.jpg" alt="Chiranal 2024: Partners"/></p>
<ul>
<li><p><strong>Faculty of Science, Palacký University, Olomouc</strong></p>
</li>
<li><p><strong>Department of Analytical Chemistry, Palacký University, Olomouc</strong></p>
</li>
<li><p><strong>Czech Chemical Society</strong></p>
</li>
<li><p><strong>Chromatography &amp; Electrophores Czech Chemical Society</strong></p>
</li>
<li><p><a href="https://lcms.cz/companies/21"><strong>Czech Society for Mass Spectrometry</strong></a></p>
</li>
<li><p><strong>Fort Science</strong></p>
</li>
<li><p><strong>ARTECA</strong></p>
</li>
<li><p><strong>TEVA</strong></p>
</li>
<li><p><a href="https://lcms.labrulez.com/companies/1"><strong>LabRulezLCMS</strong></a></p>
</li>
</ul>
<h3><strong>Sponsors Chiranal 2024</strong></h3>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Chiranal_Sponsors_2024_14389caaaf.jpg" alt="Chiranal: Sponsors 2024"/></p>
<ul>
<li><a href="https://lcms.cz/companies/37"><strong>Altium International</strong></a></li>
<li><strong>Alven Laboratories</strong></li>
<li><strong>AMEDIS</strong>/<strong>SCIEX</strong></li>
<li><strong>Bruker</strong></li>
<li><a href="https://lcms.labrulez.com/companies/168"><strong>ECOM</strong></a></li>
<li><strong>Chromservis</strong></li>
<li><strong>MERCK</strong></li>
<li><a href="https://lcms.cz/companies/176"><strong>Metrohm</strong></a></li>
<li><a href="https://lcms.cz/companies/51"><strong>Phenomenex</strong></a></li>
<li><a href="https://lcms.cz/companies/42"><strong>Pragolab</strong></a>/<strong>Thermo Scientific</strong></li>
<li><a href="https://lcms.cz/companies/43"><strong>SHIMADZU</strong></a></li>
<li><strong>Villa Labeco</strong></li>
<li><a href="https://lcms.cz/companies/50"><strong>Waters</strong></a></li>
</ul>
<h3><strong>Permanent scientific committee</strong></h3>
<ul>
<li><p><strong>Daniel W. Armstrong (US)</strong></p>
</li>
<li><p><strong>Petr Barták (CZ)</strong></p>
</li>
<li><p><strong>Petr Bednář (CZ)</strong></p>
</li>
<li><p><strong>Salvatore Fanali (IT)</strong></p>
</li>
<li><p><strong>Bohuslav Gaš (CZ)</strong></p>
</li>
<li><p><strong>Václav Kašička (CZ)</strong></p>
</li>
<li><p><a href="https://lcms.cz/articles?search=Karel+Lemr"><strong>Karel Lemr (CZ)</strong></a></p>
</li>
<li><p><strong>Wolfgang Lindner (AT)</strong></p>
</li>
<li><p><strong>Eva Smolková (CZ)</strong></p>
</li>
<li><p><strong>Zdeněk Stránský (CZ)</strong></p>
</li>
<li><p><strong>Juraj Ševčík (CZ)</strong></p>
</li>
<li><p><a href="https://lcms.cz/articles?search=Franti%C5%A1ek+%C5%A0vec"><strong>František Švec (CZ)</strong></a></p>
</li>
<li><p><strong>Eva Tesařová (CZ)</strong></p>
</li>
</ul>
<h5><strong>Contacts</strong></h5>
<ul>
<li><p><strong>Dr. Ondřej Kurka</strong> (phone: +420 585 634 450, email: ondrej.kurka@upol.cz)</p>
</li>
<li><p><strong>Assoc. Prof. Petr Bednář</strong> (phone: +420 585 634 403, email: petr.bednar@upol.cz)</p>
</li>
</ul>


Advances in Chromatography and Electrophoresis & Chiranal 2024 (Programme)

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 16th September 2024? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring to you applications, technical notes, posters, and other documents by <strong>Agilent Technologies</strong>, <strong>Shimadzu</strong>, <strong>Thermo Fisher Scientific,</strong> <strong>Waters Corporation</strong>,<strong> Metrohm</strong>, and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/313"><strong>Galochrom</strong></a>!</p><h4><strong>1. Galochrom: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Chiral_ION_QN_and_Chiral_ION_QD_Brochure_10c44e1448.pdf"><strong>Galochrom Chiral ION-QN &amp; Chiral ION-QD</strong></a></h4><ul><li>Brochure</li></ul><h5><strong>Weak Anion-Exchanger (WAX) stationary phases for reliable chiral separation of acidic compounds</strong></h5><p><strong>Transform Your Chiral Analysis</strong></p><p>Weak Anion-Exchanger (WAX) stationary phases for reliable chiral separation of acidic compounds One of the main challenges of today’s liquid chromatography is chiral separation of ionised and ionisable analytes, e.g., organic acids, amino acids, N-protected amino acids, amino acid mimics, etc. The most efficient approach to purify and resolve such troublesome racemates utilises ion exchange-type stationary phases that facilitate strong interactions between the chiral selector and the analyte.</p><p>Galochrom introduces its own generic series of weak anion-exchangers, based on tert-butyl-carbamoylated Cinchona alkaloids quinine (QN) and quinidine (QD). These chiral phases have demonstrated excellent selectivity and improved peak resolution, resulting in highly accurate and replicable analyses. Our innovative manufacturing and strict quality control ensure that these generic columns not only outperform commercial alternatives, but also meet your technical requirements.</p><p><strong>Chiral </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1241"><strong>ION-QN</strong></a><strong> &amp; Chiral </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1240"><strong>ION-QD</strong></a><strong> represent the most efficient approach to purify and resolve even the most challenging mixtures.</strong></p><h5><strong>Applications</strong></h5><p>Chiral ION-QN &amp; Chiral ION-QD are ideal for enantioseparations of:</p><ul><li><strong>N-protected amino acids</strong></li><li><strong>Aminophosphonic &amp; Aminosulfonic acids</strong></li><li><strong>Lactic &amp; Thiolactic acids</strong></li><li><strong>Clenbuterol &amp; Thyroxine</strong></li></ul><h4><strong>2. Metrohm: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_eis_004_download_4e13c9c1a9.pdf"><strong>Electrochemical Impedance Spectroscopy - Equivalent circuit models</strong></a></h4><ul><li>Technical note</li></ul><h5><strong>Electrochemical Impedance Spectroscopy</strong></h5><p><strong>Part 4 – Equivalent circuit models</strong></p><p>The circuit elements which are described in Application Note AN-EIS-003 can be combined in series and in parallel to build equivalent circuit models which can then be used to model the various phenomena occurring at the electrochemical interface.</p><p>This seven-part series introduces EIS and covers basic theory, experimental setups, common equivalent circuits used for fitting data, and tips for improving the quality of the measured data and fitting. This Application Note (part 4) provides example equivalent circuit models produced from the elements discussed in part 3. These models are among the most commonly encountered within electrochemistry research. The associated Nyquist plot is also shown for each model discussed.</p><p><strong>CONCLUSIONS</strong></p><p>This Application Note shows how electrical elements can be arranged in order to build both simple and more complex equivalent circuits that can be used for fitting EIS data. The resulting Nyquist plots for all the equivalent circuits are shown as well.</p><h4><strong>3. Thermo Fisher Scientific: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/wp_002641_ic_dionex_inuvion_ionic_small_polar_compounds_wp002641_na_en_9c0213a96b.pdf"><strong>Developing an ion chromatography system</strong></a></h4><ul><li>Technical note</li></ul><p><strong>Introduction</strong></p><p>Ion chromatography (IC) has become essential for analyzing ionic and small polar compounds. It has been applied in many areas, ranging from the analysis of drinking water, food and beverages, and cooling water to lithium-ion battery research, process quality assurance, and the presence of corrosives in liquified petroleum gas.</p><p>IC can trace its origins to work done at what would become Dionex Corporation in the mid-1970s with the introduction of the first ion chromatograph in 1975, the Dionex™ Model 10 IC system. This system had the essential elements of an IC system: a pump, separating column, eluent suppressor, conductivity detector, and data recorder (Figure 1).</p><p>This basic system provided adequate data, but several factors restricted sample throughput and required a considerable investment in labor and significant expertise to maintain. Column capacity and packed bed suppressor capacity were limited, and the instrument configuration was inflexible, constraining the type of samples that could be analyzed. Offline suppressor column regeneration was needed, the pressure tolerance was low, and the background conductivity was comparatively high. Furthermore, manual peak integration was required, eluent usage was significant, and there was substantial run-to-run variability. Since the Dionex Model 10 IC system, many innovations have been developed and incorporated into new systems to address these deficiencies. Additional advancements have enhanced robustness, reliability, user-friendliness, functional flexibility, and integrated data analysis. Ideally, an ion chromatograph will meet current customer demands while being adaptable to address potential future analytical needs.</p><p><strong>Conclusion</strong></p><p>The progression from the Dionex Model 10 IC system to the sophisticated Dionex Inuvion IC system highlights the significant advancements that have been made in the field of IC. These advancements have not only addressed previous limitations but also introduced new features and capabilities that significantly enhance the performance, reliability, and user-friendliness of IC systems. As a result, the Dionex Inuvion IC system represents the current pinnacle in the ongoing development of IC technology, setting a new standard for what can be achieved in the analysis of ionic and small polar compounds.</p><p>The Dionex Inuvion IC system is the result of state-of-the-art techniques and processes used to manufacture, design, and optimize essential hardware components such as pumps, instrument electronics, range-free detectors, and conductivity cells. This integration of advanced technology has resulted in a system that is not only reliable and robust but also flexible and user-friendly.</p><p>As we look to the future, the field of ion chromatography will continue to evolve with new challenges and opportunities on the horizon. The Dionex Inuvion IC system is well-positioned to meet these challenges, providing laboratories with a reliable and efficient solution for their analytical needs. In conclusion, the Dionex Inuvion IC system represents a significant achievement in ion chromatography, solidifying Thermo Fisher Scientific's position as a leader in the industry.</p><h4><strong>4. Waters Corporation: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/2024_asms_alelyunas_cellculturemediabioprocessing_27380a2953.pdf"><strong>A High Throughput LC-MS Method for Cell Culture Media Nutrient and Metabolite Analysis Supporting Upstream Bioprocessing</strong></a></h4><ul><li>poster / <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/296"><strong>ASMS</strong></a></li></ul><p><strong>INTRODUCTION</strong></p><p>A 9-minute rapid and direct liquid chromatography and mass spectrometry (LC-MS) analysis method for cell culture media (CCM) nutrients and metabolites covering 220+ compounds is described. The method is based on reversed phase chromatography where amino acids are directly detected without derivatization. This rapid method reduces turn-around time for monitoring and decision making using previously established user-friendly data review workflows and access to multivariate data analytics (MVDA). The method has been applied for the qualitative and quantitative determination of cell culture media nutrient and metabolite analysis in commercial cell culture media and spent media from process optimization in cNISTmAb production using NISTCHO cells. Monitoring of glucose directly from media using this method was also investigated and described.</p><p><strong>CONCLUSION</strong></p><ul><li>A rapid throughput method based on 9 min data acquisition is described for cell culture media nutrient and metabolite monitoring.</li><li>The method uses less than 10 μL of spent media sample and has coverage of 220 compounds.</li><li>Comparison with the previous 20 min method suggests the same high-quality data and method robustness, even when the analysis time is shortened by 50%.</li><li>Combination of the rapid throughput method with Waters bioprocess walk-up solution<sup>2</sup> can enable analytical scientist and bioprocess engineers to obtain high quality data easily and routinely for upstream bioprocess optimization.</li></ul><h4><strong>5. Shimadzu: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_00767_en_135939ebe6.pdf"><strong>Automatic Optimization of Gradient Conditions by AI Algorithm for Impurity Analysis</strong></a></h4><ul><li>Application</li></ul><p><strong>User Benefits</strong></p><ul><li>The AI algorithm of LabSolutions MD can automatically optimize gradient conditions to greatly reduce labor of LC method<br>development.</li><li>Anyone can optimize gradient conditions, regardless of their experience in chromatography.</li><li>Gradient conditions that meet the resolution criteria for specified peaks are automatically searched (e.g., principal component and its related impurities).</li></ul><p><strong>Introduction</strong></p><p>In the typical LC method development, the process begins with “preparation” which includes mobile phase preparation, column installation, and creation of analysis schedules, then the analysis is started. After that, the acquired data is analyzed and “preparation” for the subsequent analysis is carried out, followed by starting the next analysis again. The method development progresses by repeating these processes, but in addition to the significant time required to repeatedly create analysis schedules, expertise in chromatography is necessary to explore optimal conditions based on data analysis. In other words, typical method development requires “human intervention”. Therefore, eliminating human involvement and automating such method development processes would be desirable to improve labor efficiency. This article introduces an example of automatic optimization of gradient conditions to separate montelukast (a small molecule drug) and its related impurities using LabSolutions MD (<a target="_blank" rel="noopener noreferrer" href="https://www.shimadzu.com/an/sites/shimadzu.com.an/files/pim/pim_document_file/technical/technical_reports/22105/c190-e309.pdf">Technical Report C190-E309</a>), a dedicated software for supporting method development.</p><p><strong>Conclusion</strong></p><p>Automatic optimization of gradient conditions using AI algorithm of LabSolutions MD was applied to montelukast and its related impurity. As a result, gradient conditions that met the criteria (“resolution for montelukast and related impurity &gt; 3.0” and “elution time of the last peak &lt; 15 minutes”) were successfully explored. This result indicates that significant labor saving in method development can be expected by LabSolutions MD. This article introduces an automatic optimization of gradient conditions in method development while LabSolutions MD also supports a series of workflow of method development, including the screening phase and robustness evaluation phase. For details, please refer to the Technical Report “<a target="_blank" rel="noopener noreferrer" href="https://www.shimadzu.com/an/sites/shimadzu.com.an/files/pim/pim_document_file/technical/technical_reports/14629/c190-e284.pdf">Efficient Method Development Based on Analytical Quality by Design with LabSolutions MD Software (C190-E284)</a>”.</p><h4><strong>6. Agilent Technologies: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_polar_nitrosamines_1260_infinity_ii_multisampler_5994_7589en_agilent_22cb1563ee.pdf"><strong>Improved LC/MS Performance to Determine Polar Nitrosamines Using the Agilent 1260 Infinity II Hybrid Multisampler</strong></a></h4><ul><li>Application</li></ul><p><strong>Abstract</strong></p><p>This application note demonstrates the use of the Agilent 1260 Infinity II Hybrid Multisampler for the analysis of polar nitrosamines injected from organic solvents of high elution strength. The 1260 Infinity II Hybrid Multisampler is used under optimized feed speed conditions to trap and enrich the polar nitrosamines on the column. This avoids breakthrough and provides better peak shapes with more reliable quantification and improved detection limits.</p><p><strong>Introduction</strong></p><p>With the chemical synthesis of active pharmaceutical ingredients (APIs), impurities occur during chemical reactions. Genotoxic impurities (GTIs) are especially important because of their carcinogenic potential. To control the amount of GTIs, the U.S. Food and Drug Administration and the European Medicine Agency released guidelines for the maximum intake of GTIs with the daily dose of a drug depending upon the duration of application. According to these guidelines, the exposure to an individual genotoxic impurity must be below 1.5 μg/day for an application time longer than ten years.<sup>1</sup> In 2018, the drug Diovan was withdrawn from the market due to an increased amount of the nitrosamine impurity N-nitrosodimethylamine (NDMA) in the included API valsartan.<sup>2,3</sup> This application note describes the results achieved by the Agilent 1260 Infinity II hybrid injector for the analysis of nitrosamines injected from high organic solvents. The peak performance, like area precision, linearity, and sensitivity, will be discussed.</p><p><strong>Conclusion</strong></p><p>This application note describes the comparative measurement of nitrosamines using the Agilent 1260 Infinity II Hybrid Multisampler in feed injection mode and classical flow through injection mode. With the application of an optimized feed injection method, highly polar nitrosamines in a solvent of high eluting strength can be separated with good peak shapes. With classical flow through injection, massive peak broadening or even breakthrough were observed. Peak area RSD and LOQs are typically lower by a factor of 3 to 5 with feed injection.</p>

News from LabRulezLCMS Library - Week 38, 2024

Synthesis and absolute configuration of cyclic synthetic cathinones derived from α-tetralone

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The Faculty of Pharmacy, with its seat in the town of Hradec Králové, was established by a government decree in 1969. This Faculty of Pharmacy has continued in the old and long-time tradition of the education of pharmacy at Charles University.

Faculty of Pharmacy, Charles University

Symposium belongs to the periodical meetings of specialists working in the field of separation methods.

Advances in Chromatography and Electrophoresis & Chiranal

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Synthesis and absolute configuration of cyclic synthetic cathinones derived from α-tetralone

News from LabRulezLCMS Library - Week 38, 2024

Advances in Chromatography and Electrophoresis & Chiranal 2024 (Programme)

We are trying to succeed with the spin-off company Galochrom

6th STARSS Conference on Separation Science (Program)

Advances in Chromatography and Electrophoresis & Chiranal 2022 - Programme

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Understanding quantification algorithms

36th MassSpec Forum Vienna 2026

Analytical Strategies for Peptide & Protein Therapeutics

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High-throughput stability screening strategies for antibody formulation

Big Scale, Big Wins: Prep Chromatography Explained

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