A fast HPLC-UV method was optimized to quantify trigonelline, 5-CQA, and caffeine in multiple Arabica cultivars. The method proved robust and highlighted major cultivar and roasting-related changes.

Learn how to minimize peak tailing when analyzing basic compounds on type A silicas. Explore pH adjustment, mobile phase modifiers, and modern type B silica columns for sharper, more symmetrical peaks.

Learn how to develop robust HPLC methods for peptide analysis using systematic screening of column chemistry, pH, and gradients for optimal selectivity and reproducibility.

Column contamination is a leading cause of shortened HPLC column lifetime, leading to high back pressure. Learn why it happens and how to prevent blocked frits with simple precautions.

Struggling with peak tailing of basic compounds in RP-LC using type A silica columns? Discover how low-pH buffers and silanol suppressors can improve peak shape—plus why type B silica is a better choice.

New end-capping technologies can significantly improve the analysis of compounds that were once considered challenging.

Scientists at IOCB Prague created an eco-friendly method to recycle rare earths like neodymium from magnets, offering a sustainable alternative to mining and boosting EU & US raw material security.

How can capacity factors drive method robustness?

The effects of gradient on buffer capacity