Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 25th August 2025? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you poster by Agilent Technologies / ASMS, brochure by Shimadzu, other document by Thermo Fisher Scientific and application note by Waters Corporation!<h3>1. Agilent Technologies / ASMS: Highly Sensitive Quantitation of N-Nitroso Timolol Impurity in Timolol API using the Agilent <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=6495D+LC%2FTQ">6495D LC/TQ</a></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/ps_6495d_nitrosamines_asms_2025_mp_548_en_agilent_7c9b0b32ef.pdf">Full PDF for download</a></li></ul>Nitrosamines are of concern as most of them have been reported to be potent mutagens in rodents and are potential carcinogens. Therefore, it is important to control these impurities at or below the stipulated specification limits. API-derived complex nitrosamines are known as nitrosamine drug substance-related impurities (NDSRIs). Multiple recalls of pharmaceutical drug products due to the presence of NDSRIs have drawn the attention of regulators and manufacturers. By considering the complexity of the global pharmaceutical supply chain, manufacturers must be more diligent to protect consumers by screening their APIs and drug products for the presence of NDSRIs. NDSRIs can be formed from both secondary and tertiary amines. Secondary amines can easily undergo nitrosation in the presence of trace amounts of acid. Timolol is beta-blocker class of drug used for hypertension. N-nitroso timolol is an API specific nitrosamine impurity. For most NDSRIs, analytical methods are not yet available, so manufacturers will need to develop, qualify, and validate a method that is suitable for the sample matrix and specific for the nitrosamines in question.The analytical method developed must be sensitive enough to quantify the nitrosamines of interest down to a level that corresponds to 10% of specification limit.In this poster we highlight a highly specific, sensitive, and reproducible method that was developed for the quantitation of N-nitroso Timolol impurity in Timolol drug substance using the 6495D triple quadrupole LC/MS (LC/TQ) coupled to the 1290 Infinity II LC.<h4>Experimental</h4><h5>LC configurations and parameters:</h5><ul><li>Instrument: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=6495D+LC%2FTQ">Agilent 6495D LC/TQ</a> coupled to <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=10&amp;search=1290+Infinity+II+LC">1290 Infinity II LC</a></li><li>Analytical Column: Agilent Infinity Lab Poroshell PhenylHexyl, 150 × 3.0 mm, 2.7 µm, p/n, 693975-312</li></ul><h4>Conclusions</h4>A highly sensitive and robust multiple reaction monitoring (MRM) method was developed utilizing the Agilent 6495D LC/TQ to quantify N-nitroso timolol impurity in timolol API. &nbsp;<ul><li>The chromatographic method provided desirable separation between the impurity and the API to avoid interference.&nbsp;</li><li>The developed method demonstrated excellent linearity over the range of 5 to 1500 pg/mL (0.5 to 150 ppb with 100 mg/mL API load) with an R2 value greater than 0.9990&nbsp;</li><li>The LOD and LOQ values achieved were 2 and 5 pg/mL respectively for targeted NDSRI&nbsp;</li><li>The method provided recovery between 90 and 110%, which is within the acceptance criteria</li></ul><h3>2. Shimadzu: Liquid Chromatograph Mass Spectrometer <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=LCMS-8065XE">LCMS-8065XE&nbsp;</a></h3><ul><li>Brochure</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/c146_e500_526bbea8e9.pdf">Full PDF for download</a></li></ul><h5>Shimadzu LCMS-8065XE: Next-Generation Triple-Quadrupole Performance</h5>Shimadzu introduces the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=1&amp;search=LCMS-8065XE">LCMS-8065XE</a>, a triple-quadrupole liquid chromatograph mass spectrometer designed to set new standards in sensitivity, speed, and robustness. Built on Shimadzu’s philosophy of “Contributing to Society Through Science and Technology,” the system integrates advanced hardware with Analytical Intelligence software, ensuring both expert-level optimization and user-friendly operation. This balance of evolved, efficient, and exact capabilities makes the LCMS-8065XE a powerful solution for modern laboratories.<h5>Breakthrough Ionization and Detection Technologies</h5>At the heart of the system are innovations such as the StreamFocus ESI probe and IonFocus technology, which enhance ion intake, reduce gas consumption, and eliminate contamination without compromising sensitivity. Combined with the refined UFsweeper IV collision cell, the instrument achieves exceptional signal transmission and robust long-term stability. Demonstrated by ultra-trace PFAS detection at parts-per-trillion levels, the LCMS-8065XE ensures reliable results even under continuous high-throughput conditions.<h5>Ultra-Fast Mass Spectrometry with UF Technologies</h5>Shimadzu’s proprietary UF technologies—including UF-Qarray II, UF-Lens II, and UF-MRM—deliver unmatched speed and sensitivity. Capable of 5 msec polarity switching, up to 555 MRM channels per second, and scanning at 30,000 u/sec, the system supports complex multi-component analyses with precision. Intelligent maintenance features, such as quick desolvation line replacement without breaking vacuum, further minimize downtime and maximize laboratory productivity.<h5>Smart Software, Sustainability, and Seamless Integration</h5>The LCMS-8065XE is complemented by Peakintelligence AI algorithms, LabSolutions Connect MRM, and LabSolutions Insight, enabling automated tuning, optimized methods, and reliable peak integration with reduced manual review time. Sustainability is also central: ecology mode reduces power consumption by up to 31%, while integration with Shimadzu’s Nexera UHPLC system improves throughput and reduces resource use. Features like FlowPilot smart flow control and automatic bubble detection ensure uninterrupted performance, extending system and column lifetimes while cutting operational costs.<h3>3. Thermo Fisher Scientific: Accucore columns Resolve all your impurities without feeling the pressure</h3><ul><li>Other document</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/fl_002099_ccs_lc_columns_accucore_C18_HILIC_phenyl_fl002099_na_en_db7abbabd7.pdf">Full PDF for download</a></li></ul><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=48&amp;manufacturerItems=3&amp;search=accucore">Thermo Scientific™ Accucore™ columns </a>bring UHPLC-level performance to both HPLC and UHPLC systems, even when pressure capabilities are limited. With 14 distinct chemistries, they provide an extensive toolbox for successful method development and robust method transfer. Built on decades of innovation in stationary phase design, Accucore columns ensure reliable performance across diverse analytical challenges.<h5>Comprehensive Chemistry Portfolio for Any Separation Need</h5>The Accucore family includes multiple column chemistries tailored to different selectivity requirements. Hydrophobic options like <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/427">C18</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/428">C8</a>, and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1990">C4</a> deliver classic reversed-phase separations, while polar-modified C18 (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/430">Polar Premium</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/429">aQ</a>) enhances retention of acidic and basic compounds. Phenyl-based phases such as <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/424">Biphenyl</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/431">Phenyl-Hexyl</a>, and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1985">PFP</a> provide orthogonal selectivity for aromatic and polar analytes, and specialty hydrophobic columns (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/426">RP-MS</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1988">C30</a>) expand capabilities for structural isomer separation. For highly polar compounds, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1987">HILIC</a> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1989">Amide-HILIC</a> chemistries enable reliable retention and resolution.<h5>Optimized for Speed, Sensitivity, and Versatility</h5>Engineered with advanced particle design, Accucore columns offer rapid separations with high resolution and reproducibility. Their versatility makes them suitable for a wide range of applications, from pharmaceutical impurity profiling to food and environmental analysis. By offering both routine and specialty phases, the columns allow analysts to fine-tune selectivity and maximize productivity without compromising robustness.<h5>Trusted Innovation and Seamless Integration</h5>As part of Thermo Fisher Scientific’s portfolio, Accucore columns are backed by extensive expertise in HPLC method development and transfer. Their design supports high-throughput workflows, smooth integration into existing systems, and consistent results across laboratories. Whether the need is for routine testing or complex separations, Accucore columns deliver dependable performance with the flexibility to resolve impurities efficiently and confidently.<h3>4. Waters Corporation: Temperature Control Using Waters LC Purification Systems with Timberline's Column and Mobile Phase Heaters: Optimizing Preparative Performance&nbsp;</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720008874_en_6514d1a04e.pdf">Full PDF for download</a></li></ul><h4>Benefits&nbsp;</h4><ul><li>Minimizing method redevelopment at the prep scale improves process efficiency and reproducibility, ensuring predictable and reliable outcomes for isolating small and large molecules, such as oligonucleotides and peptides.&nbsp;</li><li>Elevated temperature reduces system backpressure and enables one to consider using of sub-5 µm Waters™ preparative Optimum Bed Density (OBD™) columns to increase productivity through faster separations, increased loading capacity, lower solvent consumption, shorter fraction dry-down times, and reduced waste disposal costs.&nbsp;</li><li>Enabling the substitution of acetonitrile with methanol provides a more environmentally sustainable and economically efficient purification process.</li></ul>Although temperature control is widely implemented in analytical HPLC to improve separations and ensure consistent and reliable outcomes, temperature is seldom controlled or used as a parameter for manipulating chromatography at the preparative scale for two reasons. First, high flow rate separations occur at the temperature of the incoming solvent and efficient heating of the mobile phase entering the column is challenging at these high flow rates. Second, large diameter columns cannot be effectively heated from the outside simply by using insulation, heated blankets or ovens. Ideally, analytical separations developed with temperature control should be scaled to prep at the same temperature to eliminate variability in the chromatography which can make the target identification and isolation ambiguous or lead to product loss.&nbsp;When used with the Waters AutoPurification System, Timberline’s column and mobile phase heaters provide precise and consistent temperature control for preparative separations, allowing easier scale-up from UPLC/UHPLC to preparative methods. This integrated approach minimizes the need for method redevelopment, improves reproducibility, enhances hydrophobic compound solubility and boosts operational efficiency, which is particularly valuable in small molecule isolations and biological applications such as oligonucleotide and peptide purification. Temperature control also enhances flexibility in purification workflows, supporting green chemistry initiatives by enabling the substitution of acetonitrile with methanol. Elevated temperature reduces system backpressure, allowing the usage of sub-5 µm particles in larger-dimension preparative columns (e.g., 10 mm ID), which may increase loading capacity, minimize run-times, reduce solvent consumption, shorten dry-down times and lower waste disposal costs. This technical brief illustrates that comparable purification results can be achieved using methanol with sub-5 µm 10 mm ID columns, an approach that is both environmentally sustainable and economically viable. It also illustrates the precision and stability of temperature control across various flow rates and mobile phase compositions, showcasing the reliability of this solution.<h4>Conclusion&nbsp;</h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=5&amp;search=Timberline%E2%80%99s+TL-105+D+column">Timberline’s TL-105 D column</a> oven configured with the Waters AutoPurification System provides precise and consistent temperature control when scaling from analytical to preparative methods. This significantly reduces the need for method redevelopment at the prep scale, enhances chromatographic efficiency and reproducibility and delivers predictable and reliable outcomes for various applications including small and large molecules such as oligonucleotides and peptides isolations.This combined solution supports the growing demand for green chemistry by enabling the substitution of acetonitrile with methanol. Enhanced temperature control also lowers system backpressure, allowing the use of smaller particle size, larger dimension Waters preparative OBD columns. This results in faster separations, increased loading capacity, reduced solvent consumption, shorter fraction dry-down times, and lower waste disposal costs. These benefits contribute to a more environmentally sustainable and economically efficient purification process.

News from LabRulezLCMS Library - Week 35, 2025

<h3>Smarter sample preparation for LC-MS: from simplicity to selectivity</h3>In any toxicology lab, sample preparation is more than a preliminary step—it’s the foundation for accuracy, reproducibility, and workflow efficiency. In a recent <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/3978">webinar</a>, we explored a range of sample preparation strategies—from minimal processing to highly selective techniques—with additional insights shared by Dr. William Clarke, PhD, MBA, DABCC, Professor of Pathology and Deputy Director of Quality and Regulatory Affairs at Johns Hopkins University School of Medicine. These discussions build on our latest white paper, “<a target="_blank" rel="noopener noreferrer" href="https://assets.thermofisher.com/TFS-Assets/CMD/Reference-Materials/wp-000861-lsms-clinical-tox-explorer-toxicology-wp000861-na-en.pdf">Context matters: selecting LC-MS sample preparation methods for LC-MS/MS in clinical research and toxicology applications</a>“, authored by Prof. Alain Verstraete (University Hospital Ghent). The paper highlights commonly used LC-MS sample preparation methods and provides practical guidance to help laboratories select the most appropriate approach for their needs.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Sample_Preparation_Strategies_Demystified_Choosing_the_Right_Fit_for_Your_Toxicology_Workflow_5_722a357221.jpg" alt="Thermo Fisher Scientific: Sample Preparation Strategies Demystified: Choosing the Right Fit for Your Toxicology Workflow: Thermo Scientific SOLAμ SPE Plates—Explore SOLA." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Sample_Preparation_Strategies_Demystified_Choosing_the_Right_Fit_for_Your_Toxicology_Workflow_5_722a357221.jpg 245w," sizes="100vw" width="700" height="340"><h3>The power of simplicity: dilute-and-shoot and protein precipitation</h3>For relatively clean matrices like urine, a dilute-and-shoot approach can be effective. This method involves minimal handling and is low-cost but generally best suited for low-protein samples to avoid ion suppression or instrument issues. For samples rich in proteins, such as plasma, serum, or whole blood, protein precipitation (PPT) is a common and straightforward method. It uses organic solvents—often methanol—to denature and precipitate proteins. After incubation at low temperatures and centrifugation, the supernatant is collected and may be further diluted. Additives like zinc sulfate can be added to enhance protein precipitation or aid cell lysis. While PPT is less selective, it effectively reduces matrix complexity and is compatible with <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/forensics/forensic-toxicology/tox-explorer-collection-lc-ms.html">robust LC-MS instrumentation</a>. As a plug-and-play approach, the <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/forensics/forensic-toxicology/tox-explorer-collection-lc-ms.html">Thermo Scientific Tox Explorer</a> method, with over 2,000 compounds in its library, includes sample preparation recommendations for varying matrices, including dilute-and-shoot and protein precipitation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Sample_Preparation_Strategies_Demystified_Choosing_the_Right_Fit_for_Your_Toxicology_Workflow_4_658266d072.jpg" alt="Thermo Fisher Scientific: Sample Preparation Strategies Demystified: Choosing the Right Fit for Your Toxicology Workflow: Tox Explorer compound library shown in mzVault." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Sample_Preparation_Strategies_Demystified_Choosing_the_Right_Fit_for_Your_Toxicology_Workflow_4_658266d072.jpg 245w," sizes="100vw" width="1024" height="480">Enhancing selectivity: Phospholipid Removal, Liquid-Liquid Extraction (LLE), Supported Liquid Extraction (SLE), and Solid-Phase Extraction (SPE)When higher selectivity and sensitivity are required, extraction-based methods are employed:<ul><li>Phospholipid Removal uses commercial solutions to reduce matrix components that can cause ion suppression. Though relatively simple, it requires some specialized materials and does not concentrate analytes.</li><li>Liquid-Liquid Extraction (LLE) separates analytes based on their solubility differences between aqueous biological fluids and immiscible organic solvents. This technique removes many matrix interferences but is labor-intensive and challenging to automate.</li><li>Supported Liquid Extraction (SLE)&nbsp;improves upon LLE by using a porous support to retain the aqueous phase, allowing nonpolar analytes to partition into an organic solvent, which can be collected more efficiently as flow-through. SLE offers cleaner extracts and better reproducibility, making it more compatible with higher-throughput workflows.</li><li>Solid-Phase Extraction (SPE) selectively retains analytes on sorbent phases based on chemical characteristics such as polarity or charge. Impurities are washed away, and analytes are eluted with strong organic solvents, often followed by drying and reconstitution. SPE improves consistency and reduces matrix effects, especially in complex samples or high-throughput environments. Various sorbents, including ion exchange types, can be selected, or even combined, depending on analyte chemistry.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Sample_Preparation_Strategies_Demystified_Choosing_the_Right_Fit_for_Your_Toxicology_Workflow_2_fee3c7bdd8.jpg" alt="Thermo Fisher Scientific: Sample Preparation Strategies Demystified: Choosing the Right Fit for Your Toxicology Workflow: Common sample preparation methods for LC-MS featured in table 1 of white paper 000861." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Sample_Preparation_Strategies_Demystified_Choosing_the_Right_Fit_for_Your_Toxicology_Workflow_2_fee3c7bdd8.jpg 245w," sizes="100vw" width="1000" height="548"><h3>Unlocking efficiency: how automation is transforming sample preparation</h3>As labs face staffing shortages and increasing sample volumes, automation is becoming essential. Solutions from providers like Hamilton and Tecan help streamline sample preparation tasks, including pipetting, plate transfers, and offline SPE. This can dramatically reduce hands-on time and standardize processes. In the webinar, Dr. Clarke shares that one lab, for example, was able to cut total sample preparation time in half—from six hours to three—and reduce hands-on analyst time from three hours to just 10 minutes by using a Tecan platform with SPE plates. Each of the extraction methods discussed can be readily programmed onto these liquid handling robots. Still, automation brings new challenges—like programming robots, accounting for sample variability, managing liquid classes (e.g., whole blood vs. serum), and replicating the precision of skilled human pipetting, which remains difficult to fully automate and underscores the continued importance of human expertise<h3>Emerging trends: dried blood spots and microsampling</h3>Dried blood spots (DBS) and microsampling techniques promise minimally invasive collection and improved sample logistics. Fixed-volume devices with or without pre-punched filter cards can simplify automation and downstream analysis. However, challenges remain: hematocrit effects, uneven sample spread, storage issues, and potential for contamination or hemolysis.Capillary sampling from fingersticks may seem like a step toward decentralized testing, but it’s not without challenges—sample quality can be affected by peripheral blood differences or inconsistent collection techniques.To address some of these limitations, particularly the need for extensive sample prep for screening purposes, direct analysis methods are gaining traction. The <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-accessories/verispray-paperspray-ion-source-mass-spectrometry.html">Thermo Scientific VeriSpray PaperSpray ion source</a> offers a direct MS-based ionization approach for analyzing dried samples, eliminating the need for time-consuming sample preparation and chromatographic separation. This can be especially useful for complex matrices like whole blood, which often require more intensive extraction to remove proteins.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Sample_Preparation_Strategies_Demystified_Choosing_the_Right_Fit_for_Your_Toxicology_Workflow_3_cb6f7e0282.jpg" alt="Thermo Fisher Scientific: Sample Preparation Strategies Demystified: Choosing the Right Fit for Your Toxicology Workflow: VeriSpray paper spray ionization is a rapid and user-friendly sample introduction technique that delivers samples directly to the mass spectrometer using electrospray ionization. The process is straightforward—just add the sample and solvent to the paper." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Sample_Preparation_Strategies_Demystified_Choosing_the_Right_Fit_for_Your_Toxicology_Workflow_3_cb6f7e0282.jpg 245w," sizes="100vw" width="1024" height="271"><h3>Final takeaway: clean samples, better results</h3>The overarching message: cleaner samples drive better assay performance. Whether you’re processing high-throughput toxicology screens or quantifying small molecules and peptides, aligning your sample preparation strategy with both analytical and operational goals is essential. From simple dilute-and-shoot methods to advanced approaches or fully automated workflows, thoughtful preparation can enhance instrument uptime, improve assay accuracy, and reduce long-term operational costs. A recent technical note—linked under the SPE section in the supporting documents below—demonstrates this effectively using a large urine drug panel. Solid-phase extraction plates improved both assay robustness and accuracy. By extracting just 200 µL of sample and minimizing solvent use, the workflow successfully reduced reagent costs and waste without sacrificing data quality. As mass spectrometry continues to expand into more complex and decentralized applications, laboratories that invest in fit-for-purpose sample prep strategies will be best positioned to deliver confident, high-quality results at scale. To support these efforts, Thermo Fisher Scientific offers a variety of aligned resources, including those discussed in the webinar and this blog:<ul><li>Dilute-and-shoot: See the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_000794_lsms_tox_explorer_orbitrap_exploris_120_drugs_of_abuse_tn000794_na_en_3286411f97/tn-000794-lsms-tox-explorer-orbitrap-exploris-120-drugs-of-abuse-tn000794-na-en.pdf">Thermo Scientific Tox Explorer method for Orbitrap-based screening of 120 drugs of abuse</a> and <a target="_blank" rel="noopener noreferrer" href="https://assets.thermofisher.com/TFS-Assets/CMD/Reference-Materials/wp-000861-lsms-clinical-tox-explorer-toxicology-wp000861-na-en.pdf">this white paper highlighting the comparison of sample prep methods.</a></li><li>Protein Precipitation: <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_73168_lc_ms_drugs_of_abuse_tn73168_en_6ff028434e/tn-73168-lc-ms-drugs-of-abuse-tn73168-en.pdf">Quantification of 78 drugs of abuse in human blood by liquid chromatography – HRAM Orbitrap mass spectrometry for clinical research</a>.</li><li>Solid phase extraction (SPE): Learn more through our tox drugs of abuse <a target="_blank" rel="noopener noreferrer" href="https://assets.thermofisher.com/TFS-Assets/CMD/Technical-Notes/tn-002363-clinical-lc-ms-homestead-tn002363-na-en.pdf">Thermo Scientific Orbitrap Exploris 120 MS</a> and <a target="_blank" rel="noopener noreferrer" href="https://assets.thermofisher.com/TFS-Assets/CMD/Technical-Notes/tn-002606-lsms-clinical-toxicology-doa-tn002606-na-en.pdf">Thermo Scientific TSQ Quantis Plus MS method for clinical toxicology</a> technical notes.</li><li>Automation: See how oral fluid extraction is streamlined using automation in <a target="_blank" rel="noopener noreferrer" href="https://assets.thermofisher.com/TFS-Assets/CMD/Technical-Notes/tn-003851-cl-lcms-stellar-oral-fluid-tn003851-na-en.pdf">this technical note featuring Thermo Scientific Stellar MS.</a></li><li>Dried sample workflows: Explore simplified prep using the Thermo Scientific VeriSpray PaperSpray Ion Source in <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_73467_ps_ms_paperspray_tandem_ms_drugs_of_abuse_urine_tn73467_en_d81464d205/tn-73467-ps-ms-paperspray-tandem-ms-drugs-of-abuse-urine-tn73467-en.pdf">this technical note on urine drug analysis.</a></li></ul><blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#SamplePreparation, #ForensicToxicology, #toxicology, #lcms</blockquote><blockquote><h4>Ema Ruzic</h4>Vertical Marketing Manager, Chromatography and Mass Spectrometry Division (CMD)</blockquote>

Sample Preparation Strategies Demystified: Choosing the Right Fit for Your Toxicology Workflow

In today’s forensic and clinical toxicology labs, productivity is more than just speed—it’s about accuracy, flexibility, and the ability to keep pace with an ever-expanding universe of analytes. Whether processing high-throughput urine samples or evaluating oral fluid, labs are under pressure to deliver reliable results faster, with fewer resources, and across a wide range of complex drug panels. Thermo Fisher Scientific is addressing this challenge head-on, delivering complete LC-MS/MS workflows, solution guides, and databases that prioritize throughput and robustness without sacrificing sensitivity or selectivity. Workflows that include over 100 to 2000 drugs allow for single injection assays that mitigate the need to extract and analyze each drug class individually. The fast speed of the mass spectrometers gives users the ability to speed up gradients, leading to shorter run times.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Boosting_Productivity_in_Toxicology_Labs_7d80cfd388.jpg" alt="Thermo Fisher Scientific: Boosting Productivity in Toxicology Labs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Boosting_Productivity_in_Toxicology_Labs_7d80cfd388.jpg 245w," sizes="100vw" width="1024" height="512"><h3>A unified workflow for broad drug coverage in urine</h3>Urine remains the cornerstone matrix for drug testing, offering a long detection window for many commonly abused substances. However, with over 100 frequently tested drugs and a growing list of synthetic analogs, labs need streamlined methods capable of handling this analytical diversity. A recent method developed by application scientist Courtney Patterson using <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-sample-preparation/sample-preparation-consumables/solid-phase-extraction-consumables/sola-spe-plates-cartridges.html">Thermo Scientific SOLAµ SPE plates</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=37&amp;manufacturerItems=3&amp;search=vanquish">Thermo Scientific Vanquish UHPLC</a>, and both <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=22&amp;manufacturerItems=3&amp;search=orbitrap">Thermo Scientific HRAM Orbitrap</a> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=20&amp;manufacturerItems=3&amp;search=tsq">Thermo Scientific TSQ triple quadrupole</a> mass spectrometry platforms, delivers a compelling solution. This workflow (linked in the resources section below), utilizes both HRAM Orbitrap and triple quadrupole mass spectrometers, quantified 106 drugs—including 15 isomers—across 10 drug classes, including drugs in both positive and negative polarities, in just 7 minutes per sample. Notably, it demonstrated strong chromatographic resolution for challenging isomeric pairs like codeine/hydrocodone and methamphetamine/phentermine. Lower limits of quantitation (LOQs) ranged from 0.1 to 100 ng/mL, meeting stringent ANSI and forensic standards. Wide dynamic ranges, such as 0.5 to 5,000 ng/mL for norbuprenorphine, enabled labs to confidently report both trace and high-concentration results with minimal rework.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Boosting_Productivity_in_Toxicology_Labs_2_0e2f7432b4.jpg" alt="Thermo Fisher Scientific: Boosting Productivity in Toxicology Labs: Figure 1 Extracted ion chromatogram of 106 drugs of abuse in urine from TN002363." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Boosting_Productivity_in_Toxicology_Labs_2_0e2f7432b4.jpg 220w," sizes="100vw" width="672" height="476">What makes this method stand out is its dual-platform compatibility. The same sample preparation and chromatography approach has been validated on both high-resolution Orbitrap and triple quadrupole systems, giving laboratories the freedom to select instrumentation that best aligns with their throughput needs and budget constraints.And speaking of budgets… Be sure to explore our latest promotions, including <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/o/b/clinical-lcms-discounts.html">complete LC-MS toxicology configurations</a> and <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/mass-spectrometry/mass-spectrometer-price-affordability-programs.html">affordability programs</a>. You can aim high without overspending by:<ul><li>Investing in certified refurbished instruments at a fraction of the cost</li><li>Trading in older instruments for credit toward the latest models, regardless of manufacturer</li><li>Taking advantage of flexible leasing and financing options, including 0% interest and deferred payment plans</li></ul><h3>Meeting new demands in oral fluid testing with Stellar MS</h3>As oral fluid gains traction for workplace drug testing, roadside evaluations, and rapid screening, labs face a different set of challenges—chief among them, the need for highly sensitive and selective methods to meet SAMHSA detection thresholds. Patterson’s oral fluid workflow, powered by the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1149">Thermo Scientific Stellar mass spectrometer</a>, addresses these needs with precision in a 5-minute method. Using DPX Technology’s INTip™ SCX/WAX SPE tips for extraction and an<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/424"> Accucore Biphenyl column</a> for separation, this method quantified 31 drugs—including THC, opioids, stimulants, and cocaine—at LOQs as low as 0.25 ng/mL. The Stellar system’s targeted-MS2 (tMS2) acquisition mode delivered confident identification with full-scan MS2 spectra, resolving issues like matrix suppression common in oral fluid analysis.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Boosting_Productivity_in_Toxicology_Labs_3_146d0fde1c.jpg" alt="Thermo Fisher Scientific: Boosting Productivity in Toxicology Labs: Figure 2 Schematic of XTR tip extraction protocol with mixed mode SCX/WAX chemistry for INTip dispersive solid phase extraction followed by analysis using the Vanquish Horizon UHPLC system and Stellar Mass Spectrometer from TN003851. Artwork provided by DPX Technologies." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Boosting_Productivity_in_Toxicology_Labs_3_146d0fde1c.jpg 245w," sizes="100vw" width="867" height="375">Importantly, the method achieved baseline separation of structural isomers and provided flexibility in fragmentation, using either HCD or CID based on compound-specific needs. Ten real-world case study samples were analyzed successfully, confirming all compounds with robust retention times and fragment ion ratios.<h3>Productivity without compromise</h3>Together, these two workflows—one for urine and one for oral fluid—showcase how Thermo Fisher Scientific is redefining productivity in toxicology. By combining smart sample preparation, fast chromatography, and high-resolution mass spectrometry platforms, these solutions enable:<ul><li>Rapid turnaround times with sub-10-minute methods</li><li>High-confidence results for complex drug panels, including isomers</li><li>Flexible instrumentation to meet the needs of both high-resolution and triple quadrupole users</li><li>Regulatory readiness with LOQs meeting ANSI and SAMHSA guidelines</li></ul>In a world where toxicology labs must do more with less, Thermo Fisher’s comprehensive solutions empower teams to work faster, smarter, and with greater confidence.Learn more about how Thermo Fisher Scientific’s toxicology solutions can help your lab stay ahead: <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/forensics/forensic-toxicology.html">Forensic Toxicology</a><h3>Resources</h3><ul><li>Past blogs in this series<ul><li>Sample prep – <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6189">Sample Preparation Strategies Demystified: Choosing the Right Fit for Your Toxicology Workflow</a></li><li>Tox Explorer – <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6587">Beyond Blood: Sheffield Teaching Hospitals NHS Foundation Trust Develops HRAM-Powered Method for Post-Mortem Toxicology Analysis</a></li><li>VeriSpray – <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/6860">Advancing Drug Analysis with PaperSpray MS</a></li></ul></li><li>On-demand workshop – <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4735">Next-Generation Workflows for Small Molecule Quantitation Using the Thermo Scientific Stellar Mass Spectrometer</a></li><li>Technical note – <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/32266">Quantitation of an oral fluid drug panel including THC using the new Stellar mass spectrometer</a></li><li>Technical note – <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/32265">Utilizing HRAM Orbitrap mass spectrometry to quantitate 106 drugs of abuse in urine</a></li><li>Technical note – <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/32265">Utilizing triple quadrupole mass spectrometry to quantitate 106 drugs of abuse in urine</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/32265">Drug screening and confirmation workflows</a></li><li>Promotions<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/o/b/clinical-lcms-discounts.html">Save up to 40% on complete LC-MS toxicology configurations</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/mass-spectrometry/mass-spectrometer-price-affordability-programs.html">Mass spectrometer affordability programs</a><ul><li>Refurbished MS</li><li>Trade-in</li><li>Financing/ leasing</li></ul></li></ul></li></ul>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#ForensicToxicology #MassSpectrometry #LabProductivity<blockquote><h5>Ema Ruzic</h5><div>Vertical Marketing Manager, Chromatography and Mass Spectrometry Division (CMD)</div></blockquote>

Boosting Productivity in Toxicology Labs

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News from LabRulezLCMS Library - Week 11, 2026

Boosting Productivity in Toxicology Labs

Sample Preparation Strategies Demystified: Choosing the Right Fit for Your Toxicology Workflow

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