We are pleased to invite you to our upcoming free online webinar on June 11, 2026 focused on one of the most pressing challenges in environmental monitoring and water quality management — PFAS (Per- and Polyfluoroalkyl Substances).As regulatory frameworks continue to evolve and compliance deadlines approach, laboratories, water utilities, and environmental professionals need robust, reliable, and cutting-edge analytical solutions to stay ahead.<blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://events.teams.microsoft.com/event/91d97003-fe33-4dca-b80b-160d9f8a5655@485ca04e-6f74-4050-9764-cdb4bfa89c25">Registration HERE</a></blockquote><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/ALS_analyzy_PFAS_a9e4a87fbc.jpg" alt="ALS: Advanced PFAS Analytical Services " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_ALS_analyzy_PFAS_a9e4a87fbc.jpg 245w," sizes="100vw" width="600" height="336"><h4>🎤 Our Speaker</h4>The webinar will be hosted by Jana Kováčová, Ph.D., PFAS Regional Lead for Continental Europe Environmental Division, who has dedicated the last several years to the field of PFAS analysis and determination in ALS laboratories. Jana is a recognized expert in PFAS environmental analytics and will share practical insights and the latest developments in analytical strategies to help your organization achieve and maintain compliance.<h4>🎯 What You Will Learn</h4><ul><li>Latest regulatory developments and compliance requirements for PFAS in water, including EU Drinking Water Directive 2020/2184 and its implications for water suppliers and laboratories.</li><li>Regulatory updates for surface water and groundwater — what's new and what to expect next.</li><li>Overview of advanced analytical strategies for PFAS detection and quantification in water matrices and how we analyse PFAS samples.</li><li>Key analytical challenges associated with PFAS testing — from ultra-trace detection to matrix interferences and contamination control.</li><li>The critical importance of using tested sampling containers and sample preservation to ensure reliable results.</li><li>Illustrative examples of results from real-world PFAS water analyses.</li><li>How ALS can support your needs with accredited PFAS testing services.</li></ul><h4>👥 Who Should Attend</h4>This webinar is designed for our clients, professionals, laboratory and technical staff, scientists, as well as anyone with an interest in analytical chemistry who wants to better understand the occurrence of these "forever chemicals" in the environment. &nbsp;<h4>💬 Q&amp;A Session</h4>There will be a live Q&amp;A session at the end of the webinar. You are welcome to submit your questions in advance during registration or ask them live on the day.<h4>📅 Additional Webinar Info:</h4><ul><li>Platform: Microsoft Teams (online)</li><li>Time: 10:00 CEST</li><li>Language: English</li><li>Duration: approx. 45 - 60 minutes (incl. Q&amp;A) Extras: If you are unable to attend on the day, please register and we will provide you with the recording of the webinar.</li></ul><h4>🔬 About ALS Environmental</h4>ALS is a global leader in environmental testing and analytical services, providing accredited PFAS analysis to clients across Europe and beyond. Our dedicated PFAS experts help clients navigate the complex regulatory landscape with confidence.<blockquote>For further information, please contact us on: marketing.cz@alsglobal.com</blockquote>We look forward to welcoming you to this important discussion on PFAS compliance in water.

Invitation to the ALS Webinar PFAS in Water 2026: Achieving Compliance through Advanced Analytical Strategies

Why are switchable 2D-LC workflows fit for mAb manufacturing? Discover a practical AQbD approach for monitoring charge and size variants in one hour.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Monitoring_m_Ab_Manufacturing_the_Easy_Way_with_Switchable_2_D_LC_UV_MS_689c6925a2.jpeg" alt="Thermo Fisher Scientific: Monitoring mAb Manufacturing the Easy Way with Switchable 2D‑LC-UV-MS." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Monitoring_m_Ab_Manufacturing_the_Easy_Way_with_Switchable_2_D_LC_UV_MS_689c6925a2.jpeg 245w," sizes="100vw" width="1024" height="512">Lost material. Delayed production timelines. Reworks across teams.If you’re working with monoclonal antibodies (mAbs) or other biopharmaceuticals, you already know the challenge: biological reactions are variable.Unlike traditional small-molecule drugs, with simpler and more predictable chemical structures, biotherapeutics exist in a dynamic state of microheterogeneity — no two batches are ever truly identical.Whether undergoing production, processing, or storage, these compounds often undergo unwanted side reactions like glycation, deamidation, charge variation, and aggregation, which can compromise product safety and efficacy.Finding robust and dependable ways to monitor these critical quality attributes (CQA) and protect therapeutic integrity is even more pressing, as regulatory agencies demand comprehensive characterizations.While analytical techniques like liquid chromatography (LC) and mass spectrometry (MS) play a fundamental role in CQA analysis, the increasing complexity of analytical tasks is forcing the requirements of instruments to grow.The good news? <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/blog/analyteguru/biopharma-boost-how-2d-lc-went-from-expert-only-to-routine/">Targeted two-dimensional LC (2D-LC)</a> workflows that can handle analytical complexity and support straightforward method development without any manual fluidic changes are already here.Let’s explore how your lab can use 2D-LC coupled with ultraviolet detection (UVD) or high-resolution accurate mass spectrometry (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=22&amp;manufacturerItems=3">HRAM-MS</a>) to monitor the CQAs of intact mAbs in real time (Figure 1).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Monitoring_m_Ab_Manufacturing_the_Easy_Way_with_Switchable_2_D_LC_UV_MS_2_8ee6b79638.jpeg" alt="Thermo Fisher Scientific: Monitoring mAb Manufacturing the Easy Way with Switchable 2D‑LC-UV-MS: Figure 1. Fluidic scheme of a 2D-LC-MS setup that supports multiple CQA analysis as well as method development without any manual replumbing." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Monitoring_m_Ab_Manufacturing_the_Easy_Way_with_Switchable_2_D_LC_UV_MS_2_8ee6b79638.jpeg 224w," sizes="100vw" width="800" height="559"><div><h4>Key takeaways:</h4><ul><li>Why mAb variants matter</li></ul>Physiochemical modifications generate diverse mAb variants — such as deamidated and aggregated species — that require monitoring to protect therapeutic efficacy and safety.<ul><li>What switchable 2D-LC brings you</li></ul>Switchable heart-cut 2D-LC workflows allow direct upstream monitoring of multiple CQAs during the production cycle from cell culture samples.<ul><li>How the separation works</li></ul>The mAb is purified from cell culture samples by protein A (ProA) affinity in the first dimension (1D). A high-performance strong cation exchange (SCX) column applied in the second dimension (2D) facilitates the separation of mAb charge variants or serves as a trap column to focus the purified mAb fraction prior to transfer to size exclusion chromatography (SEC).<ul><li>Speed vs. depth: choosing the right detector</li></ul>UVD monitoring provides quick and simple trendlines, while the direct coupling of 2D with HRAM-MS allows deep characterizations and comprehensive insights into glycan profiles, modifications, and aggregates at the intact level.<ul><li>Why at-line monitoring matters</li></ul>Near real-time analysis of bioreactor samples enables earlier intervention, helping control material costs, improve yield, reduce off-target variants, and avoid costly downtime from rework or restarting production.</div><h3>How 2D-LC workflows help streamline mAb manufacturing and prevent costly issues</h3>Near real-time monitoring enables early intervention of issues, helping maintain product cost and quality, protect yield, and more consistent downstream performance.As best practice, you should begin monitoring product quality attributes (PQAs) from the start of the cell culture and continue daily throughout the process until your reaction reaches the target yield (Figure 2).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Monitoring_m_Ab_Manufacturing_the_Easy_Way_with_Switchable_2_D_LC_UV_MS_3_bb3e594d4a.jpeg" alt="Thermo Fisher Scientific: Monitoring mAb Manufacturing the Easy Way with Switchable 2D‑LC-UV-MS: Figure 2. mAb titer calibration curve (left) and titer determination of cell culture samples from 1D ProA-UV (right). Samples were drawn from the bioreactors over 10 days." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Monitoring_m_Ab_Manufacturing_the_Easy_Way_with_Switchable_2_D_LC_UV_MS_3_bb3e594d4a.jpeg 245w," sizes="100vw" width="768" height="282">For established reactions, you can track changes in titer, charge, or size variant profiles based on UV spectral patterns quickly adjust feeds or cell harvest timing to prevent further issues during downstream processing.Trends like the formation of new shoulders, slight peak shifts, or loss in peak intensity often signal changes in media composition, nutrient availability, pH, or process stress (Figure 3).For example:<ul><li>Emerging acidic or basic species may indicate chemical modifications, like glycation, deamidation, or other stress‑related changes.</li><li>Size variants can reflect fragmentation or aggregation driven by temperature, agitation, or hold times.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Monitoring_m_Ab_Manufacturing_the_Easy_Way_with_Switchable_2_D_LC_UV_MS_4_44dd018404.jpeg" alt="Thermo Fisher Scientific: Monitoring mAb Manufacturing the Easy Way with Switchable 2D‑LC-UV-MS: Figure 3. 2D chromatogram of ProA-SCX-SEC analysis of harvested cell culture samples drawn on days 2 and 9." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Monitoring_m_Ab_Manufacturing_the_Easy_Way_with_Switchable_2_D_LC_UV_MS_4_44dd018404.jpeg 204w," sizes="100vw" width="768" height="587"><h3>Why switchable workflows provide a simpler path to fewer surprises, faster course corrections, and more consistent mAb production</h3>Fortunately,&nbsp;you&nbsp;don’t&nbsp;need a complicated,&nbsp;manual&nbsp;multistep&nbsp;analysis pipeline to keep your mAb production schedule on track. A streamlined, switchable 2D-LC-UV-MS workflow gives you fast, actionable insights—straight from the bioreactor.&nbsp;The setup includes a <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/liquid-chromatography-lc/hplc-uhplc-systems/vanquish-simple-switch-2d-lc.html">switchable fluidic design</a> that lets you toggle between charge (SCX) and size variant analysis (SEC) without replumbing the hardware, providing complementary information.This convenient approach <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=xOzYpxWbvQw">combines cleanup and analysis in one system</a>, eliminating manual sample preparation and enabling direct analysis of cell culture supernatants from the bioreactor (Figure 4).<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Monitoring_m_Ab_Manufacturing_the_Easy_Way_with_Switchable_2_D_LC_UV_MS_5_d6dc6b0059.jpeg" alt="Thermo Fisher Scientific: Monitoring mAb Manufacturing the Easy Way with Switchable 2D‑LC-UV-MS: Figure 4. Quality attribute monitoring of mAb biosynthesis by switchable 2D-LC-UV or MS workflow." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Monitoring_m_Ab_Manufacturing_the_Easy_Way_with_Switchable_2_D_LC_UV_MS_5_d6dc6b0059.jpeg 245w," sizes="100vw" width="768" height="215"><h4>How switchable 2D-LC technology works:</h4><ul><li>1D. ProA affinity chromatography for titer determination and isolation of the mAb from host cell material and media components. The purified fraction is then sent via automated heart‑cut transfer straight into the 2D. The 1D elution conditions facilitate optimal mAb capture at the SCX column in the 2D.</li><li>ProA‑SCX to see charge drifts. Gradient elution of the SCX column in the 2D to separate acidic and basic variants from the main peak.</li><li>ProA‑SCX‑SEC to see aggregation risks. The SCX column is switched in line with the SEC column to resolve the monomer, low‑molecular‑weight (LMW), and high‑molecular‑weight (HMW) fractions. The SCX column here acts as a trap and refocusing stage, releasing the mAb into SEC in a narrow band by a step increase to high ionic strength. The trap/elute procedure is critical to yield sharp peaks downstream, as SEC cannot refocus bands, broadened by the loop volume.</li></ul>Together, these workflows support both early risk detection and informed corrective action without adding analytical complexity.<h3>UVD or MS? How to pick the right tool for the job</h3>With switchable 2D-LC workflows, you can select different detection options in the 2D: UVD or HRAM-MS (Figure 5). The fluidic setups differ slightly, but you must always use a fully volatile buffer system with the MS option.<h4>Here’s how to choose the right setup:</h4><ul><li>UVD is ideal for routine, in‑time process control of established reactions. Provides <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_004276_hplc_vanquish_heartcut_2dlc_antibody_bioproduction_processes_an004276_na_en_201cb3360f.pdf">quick and simple</a> trendlines when you need clear thresholds to act on, as well as straightforward decisions when you need day-to-day control of established processes. Like the “traffic light” for your production.</li><li>HRAM-MS is best when you need detailed native intact‑level confirmation, glycoform profiling, or deeper characterization of modifications <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_003310_hplc_vanquish_2dlc_quality_attributes_mabs_an003310_na_en_8caddf8c25.pdf">during the development stage</a> (Figure 5).</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Monitoring_m_Ab_Manufacturing_the_Easy_Way_with_Switchable_2_D_LC_UV_MS_6_d039a60ac0.jpeg" alt="Thermo Fisher Scientific: Monitoring mAb Manufacturing the Easy Way with Switchable 2D‑LC-UV-MS: Figure 5. Coupling to UVD or HRAM MS in 2D charge variant analysis. Left: quick and straightforward batch comparison with the ProA-SCX-UV. Right: ProA-SCX-HRAM MS provides deep insights into glycoforms and modifications at the intact mass level." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Monitoring_m_Ab_Manufacturing_the_Easy_Way_with_Switchable_2_D_LC_UV_MS_6_d039a60ac0.jpeg 245w," sizes="100vw" width="768" height="232"><h3>Operational benefits your entire team can trust</h3>Real-time monitoring of mAb CQAs reveals when you need to intervene and implement a bioprocess change, protecting you from the most expensive surprises: scrap costs, poor yield, off-target product, and the downtime it takes to clean and start over.From an operations perspective, the benefits of switchable 2D-LC workflows are clear and practical (Figure 6).<ul><li>A single system performs at-line cleanup and analysis, reducing manual handling and process variability.</li><li>Heart-cut transfer minimizes sample loss and contamination risk, while the switchable design provides fit-for-purpose flexibility and delivers comprehensive insight into charge and size variants within one hour.</li><li>Regular daily sampling of crude cell culture supernatants directly from the bioreactor builds reliable trendlines over time.</li><li>Operators can quickly identify batch-divergent behavior using UVD to support timely process adjustments or perform deeper molecular characterization with HRAM MS.</li><li>A standardized, switchable workflow also supports method transfer across teams and sites, reducing variability during scale-up and method transfer.</li></ul>Fewer surprises, faster course corrections, and scaling operations without expert guidance are something analysts, engineers, quality control, finance, and regulatory bodies can all get behind.<h5>Interested in using switchable 2D-LC workflows for your mAb manufacturing processes?</h5>See real bioreactor data and step-by-step setup details in our application notes today.<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_004276_hplc_vanquish_heartcut_2dlc_antibody_bioproduction_processes_an004276_na_en_201cb3360f.pdf">Switchable 2D-LC-UV workflows for routine monitoring</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_003310_hplc_vanquish_2dlc_quality_attributes_mabs_an003310_na_en_8caddf8c25.pdf">Switchable 2D-LC-MS workflows for deeper characterizations</a></li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Monitoring_m_Ab_Manufacturing_the_Easy_Way_with_Switchable_2_D_LC_UV_MS_7_d8882ed8ae.jpeg" alt="Thermo Fisher Scientific: Monitoring mAb Manufacturing the Easy Way with Switchable 2D‑LC-UV-MS: Figure 6. Illustration showing how switchable 2D-LC works." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Monitoring_m_Ab_Manufacturing_the_Easy_Way_with_Switchable_2_D_LC_UV_MS_7_d8882ed8ae.jpeg 245w," sizes="100vw" width="768" height="384"><h3>Frequently asked questions</h3><div><div><h5>1. What mAB quality control attributes should I monitor my reaction for regulatory?</h5>Some of the most important critical quality attributes of cell culture derived samples you should monitor include mAb titer, charge and size variants, and glycosylation profiles.</div><div><h5>2. What is 2D-LC?</h5>2D-LC&nbsp;is a technique that successively uses two complementary LC modes for a multi-dimensional separation. Traditionally such systems are used for additional selectivity to help resolve complex separations or to deplete interfering matrices.</div><div><h5>3. What is the benefit of using a switchable 2D-LC workflow for mAb manufacturing?</h5>Switchable heart-cut 2D-LC workflows facilitate toggling&nbsp;between size and charge variant&nbsp;analysis of the intact mAb under native conditions — directly from cell culture samples — without reconfiguring&nbsp;the&nbsp;hardware.</div><div><h5>4. Should I use UVD or MS during mAb manufacturing?</h5>UVD supports quick, efficient decisions during production progression and is ideal for monitoring well-established biosynthetic processes. HRAM-MS allows for a deep characterization and comprehensive insights into glycan profiles, modifications, and aggregates at the intact level.</div></div><blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#ChargeVariantAnalysis, #HRAM, #LiquidChromatography</blockquote>

Monitoring mAb Manufacturing the Easy Way with Switchable 2D‑LC-UV-MS

Time is running out to take advantage of discounted registration fees for the 19th Czech-Slovak Spectroscopic Conference (19th CSSC), one of the leading scientific events dedicated to spectroscopy and spectrometry in Central Europe. Researchers, students, instrument specialists, and industry professionals are invited to join the conference at the Faculty of Science, Palacký University Olomouc. The Early Bird registration period and the deadline for lecture and poster abstract submission both end on June 15, 2026.&nbsp;<h3>Registration</h3><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://cssc2026.spektroskopie.cz/registration_online">Online registration form</a></blockquote><h4>Registration Fees</h4><h5>Early (before June 15, 2026)</h5><ul><li>Participant (Member of IMMSS and SSS): 10 000 CZK&nbsp;&nbsp;</li><li>Participant (Non-member of IMMSS and SSS): 14 000 CZK&nbsp;&nbsp;&nbsp;</li><li>Students: 7 000 CZK&nbsp;</li><li>1-day registration: 5 000 CZK</li><li>Accompanying person*: 5 000 CZK</li></ul><h5>Regular (after June 15, 2026)</h5><ul><li>Participant (Member of IMMSS and SSS): 13 000 CZK&nbsp;&nbsp;</li><li>Participant (Non-member of IMMSS and SSS): 18 200 CZK&nbsp;&nbsp;&nbsp;</li><li>Students: 9 100 CZK&nbsp;</li><li>1-day registration: 6 500 CZK</li><li>Accompanying person*: 6 500 CZK</li></ul>* The accompanying person does not have access to the scientific programmeThe conference fee is calculated with VAT and includes:<ul><li>attendance to lectures and poster session</li><li>book of abstracts (electronic version)</li><li>booklet</li><li>lunches, refreshments (coffee/tea breaks)</li><li>excursion</li><li>attendance at Welcome Party and Gala Dinner.&nbsp;</li></ul>For an accompanying person, the conference fee includes the Welcome Party, the excursion and the Conference Dinner. The Conference Dinner is not included in the 1-day registration. This payment should be arranged with the Organising Committee.Accommodation is not included in the conference fee.<h3>Abstract submission</h3><blockquote>👉<a target="_blank" rel="noopener noreferrer" href="https://cssc2026.spektroskopie.cz/abstract_submission_online">ABSTRACT SUBMISSION FORM</a>Deadline for lecture/poster abstract submission is June 15, 2026</blockquote><h4>Posters</h4>Prepare your poster in English in the format A0 vertically orientated (portrait). The presence of one of the authors at the poster is requested during the poster session. Materials for mounting posters will be available on the poster panels.<h4>Lectures</h4>Prepare your presentation in English in PowerPoint of Portable Document Format (pdf), both in format 16:9 (widescreen). Invited and oral lectures takes 30 and 20 minutes (including discussion), respectively.&nbsp;<h3>TOPICS &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;</h3><h4>Czech - Slovak Spectroscopic Conference</h4><ul><li>Atomic spectrometry (AAS, ICP-MS, AFS, OES, speciation analysis, etc.)</li><li>Molecular spectrometry/spectroscopy (UV-Vis, NMR, IR, Raman, circular dichroism, etc.)</li><li>New techniques and data processing methods in IR and Raman spectroscopy, Special methods of Raman spectroscopy (resonance Raman, SERS, SERRS, TERS)</li><li>XRF spectrometry (EDS, WDS, XRF, PIXE, etc.)</li><li>Mass spectrometry &amp; hyphenated techniques</li><li>Instrumental radioanalytical methods (Gamma spectroscopy, NAA, etc.)</li><li>Laser spectroscopy and special spectroscopy techniques</li><li>Sample preparation and introduction techniques</li><li>Applications &nbsp;of atomic and molecular spectroscopy</li><li>Statistics and metrology in spectroscopy</li></ul><h4>Mössbauer Spectroscopy in Material Science</h4><ul><li>Environmental, catalytic, biologic, medical applications</li><li>Magnetism, magnetic nanoparticles, molecular magnets</li><li>Nanomaterials, nanostructures, nanocomposites, core-shell nanoparticles, thin films</li><li>Nanomaterials in biomedicine</li><li>Nuclear forward scattering in materials science</li><li>Solid-state chemistry and physics</li><li>Industrial applications, corrosion</li><li>Instrumentation, software</li><li>Earth Science, Mineralogy and Archaeology</li></ul><h4>Venue</h4><h5>Faculty of Science</h5><ul><li>Palacky University Olomouc</li><li>17. listopadu 1192/12, &nbsp;Olomouc, Czech Republic</li></ul>

19th CSSC 2026: Early Bird Registration and Abstract Submission Deadline Approaching

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