Simple Method Transfer using i-Series (LC-2050/LC-2060)

Importance of Method Transfer

Effective transfer of HPLC methods between instruments is critical for maintaining data quality and reproducibility in pharmaceutical, food, and industrial analysis. Variations in system volume, pump characteristics, and gradient delay can lead to shifts in retention time and peak resolution. A robust transfer approach saves time, reduces revalidation effort, and ensures compliance with regulatory testing protocols.

Objectives and Study Overview

This report illustrates how Shimadzu’s new i-Series integrated LC systems (LC-2050/LC-2060) and the ACTO software module can achieve seamless method transfer. Two main strategies are demonstrated:

• Correction of system gradient delay volume by matching tubing and mixers or using compatibility kits
• Automatic adjustment of the gradient start time via ACTO in LabSolutions software

Methodology and Instrumentation

A theoretical review explains gradient delay volume as the plumbing volume between solvent mixing and column inlet. Two transfer examples are presented:

• Manual volume correction between an LC-2030 Plus and LC-2050, and between an external vendor’s system, an LC-2010C HT, and the LC-2050 using a delay-volume compatibility kit
• Automatic gradient start time adjustment between an LC-2060C 3D MT and another vendor’s system using ACTO software without hardware changes

Results and Discussion

Matching system volumes yielded nearly identical chromatograms when manually correcting gradient delay. Using ACTO’s gradient start time adjustment reduced retention time errors for all sunscreen mixture peaks to below 0.5%. These results confirm that both hardware and software approaches can compensate for differences in system volume and pump delivery, ensuring consistent separations.

Benefits and Practical Applications

Key advantages of the proposed approach include:

• High reproducibility across multiple instruments and laboratories
• Time savings by avoiding method reoptimization
• Compliance with strict regulatory requirements that prohibit altering gradient programs
• Improved reliability for high-throughput and ultrafast analyses

Future Trends and Opportunities

Continued development may focus on deeper integration of software tools for automatic parameter correction, application of machine learning to predict optimal transfer settings, and expanded compatibility with third-party instruments. Real-time monitoring and digital-twin models of HPLC systems could further streamline method transfer and maintenance.

Conclusion

Shimadzu’s i-Series integrated LC systems combined with the ACTO gradient adjustment function offer a comprehensive solution for HPLC method transfer. By addressing gradient delay volume through both hardware compatibility kits and automated software corrections, laboratories can achieve consistent chromatographic performance and reduce the burden of method revalidation.