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Accurate and Sensitive LC-MS/MS Quantification of Adalimumab in Serum/Plasma: Impact of Sample Preparation on Method Performance

 

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Waters Application Notes ProteinWorks OUR SCIENTISTS Erin E. Chambers As a principal scientist, Erin has been working almost exclusively on peptide and protein bioanalysis for the last seven years, while managing small and large molecule bioanalysis and clinical research applications…
Key words
proteinworks, proteinworksdigest, digestexpress, expressarea, areapeptide, peptidekit, kittrastuzumab, trastuzumabgeneric, genericplasma, plasmadigestion, digestionftfsldtsk, ftfsldtskmurine, murineantibody, antibodyprotein, proteinsignature
[ TECHNOLOGY BRIEF ] High Sensitivity LC-MS/MS Quantification of Monoclonal Antibody Drugs in Rat Plasma Using a Standardized Sample Preparation Workflow Mary Lame, Caitlin Dunning, Steven Calciano, and Kelly Doering Waters Corporation, Milford, MA, USA Protein therapeutics, specifically monoclonal antibodies…
Key words
adalimumab, adalimumabnylawyqqkpgk, nylawyqqkpgkinfliximab, infliximabsinsathyaesvk, sinsathyaesvkquantification, quantificationtrastuzumab, trastuzumabiyptngytr, iyptngytrlloq, lloqinitial, initiallliyaastlqsgvpsr, lliyaastlqsgvpsrasqfvgssihwyqqr, asqfvgssihwyqqrglewvsaitwnsghidyadsvegr, glewvsaitwnsghidyadsvegrbrief, briefleesggglvqpggsmk, leesggglvqpggsmklliysasflysgvpsr
[ APPLICATION NOTE ] Development of a Hybrid Immunoaffinity-LC-MS/MS Method for the Quantification of Active Biotherapeutics Targeting TNF-α in Serum Caitlin Dunning and Mary Lame Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■■ ■■ High analytical sensitivity Tumor Necrosis Factor…
Key words
infliximab, infliximabimmunoaffinity, immunoaffinitynylawyqqkpgk, nylawyqqkpgkadalimumab, adalimumabbiotherapeutics, biotherapeuticssinsathyaesvk, sinsathyaesvketanercept, etanercepttnf, tnfictcrpgwycalsk, ictcrpgwycalskquantification, quantificationserum, serumhybrid, hybridapytfgqgtk, apytfgqgtkactive, activedilltqspailsvspger
A Generic Kit-Based Approach for Quantifying Monoclonal Antibody Drugs Through Direct Digestion of Discovery Study Samples Mary Lame, Hua Yang, Sherri Naughton, and Erin Chambers Waters Corporation, Milford, MA, USA A P P L I C AT I O N…
Key words
generic, genericsignature, signaturehrough, hroughkit, kitapytfgqgtk, apytfgqgtkbevacizumab, bevacizumabsinsathyaesvk, sinsathyaesvkftfsldtsk, ftfsldtskmonoclonal, monoclonalantibody, antibodyftisadtsk, ftisadtskquantifying, quantifyingproteinworks, proteinworksdirect, directdigestion
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