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Improving the Recovery of Intact Antibodies from Reversed-Phase Separations Using a BioResolve RP mAb Polyphenyl Column

 

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[ APPLICATION NOTE ] A Novel Phenyl Bonded Phase for Improved Reversed-Phase Separations of Proteins Jennifer M. Nguyen, Lavelay Kizekai, Dan Walsh, Jim Cook, and Matthew A. Lauber Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■■ ■■ Polyphenyl bonded phase…
Key words
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HIGH SENSITIVITY LC-MS PROFILING OF ANTIBODY DRUG CONJUGATES WITH DIFLUOROACETIC ACID ION PAIRING AND A HIGHCOVERAGE PHENYL-BONDED STATIONARY PHASE Jennifer Nguyen1,2, Jacquelynn Smith3, Olga V. Friese3, Jason C. Rouse4, Daniel P. Walsh1, and Matthew A. Lauber1 1 Waters Corporation, Milford,…
Key words
dfa, dfasubunit, subunitdifluoroacetic, difluoroaceticpairing, pairingabsorbance, absorbanceantibody, antibodypercent, percentadcs, adcsbeen, beentfa, tfacapaci, capacihighcoverage, highcoveragedared, dareddrug, druggradien
[ APPLICATION NOTE ] Protein Quantification in Formulation Buffer Using a BioResolve RP mAb Polyphenyl Column Hua Yang and Stephan Koza Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■■ ■■ ■■ INTRODUCTION Simple, fast separation of proteins Protein quantification is…
Key words
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[ TECHNOLOGY BRIEF ] LC-MS Profiling of IgG2 Isoforms Using Difluoroacetic Acid and Reversed-Phase Chromatography Martin De Cecco,1 Marina Solé Espeso,1 Jennifer Nguyen, 2 and Matthew Lauber 2 Sartorius Stedim BioOutsource, Glasgow, Scotland, UK; 2Waters Corporation, Milford, MA, USA 1…
Key words
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