Agilent G5523A/G Bravo Platform – Site Preparation Checklist

Significance of the topic

Proper site preparation is crucial for ensuring the Agilent G5523A/G Bravo Automated Liquid Handling Platform operates reliably, safely, and accurately throughout its service life. Adequate bench space, environmental control, utilities, and safety measures minimize installation delays, performance issues, and maintenance concerns.

Objectives and overview of the document

This checklist details all customer responsibilities and requirements before installation, including workspace dimensions, environmental specifications, power needs, laboratory supplies, and safety provisions. The goal is to guide laboratories in achieving a seamless setup and optimal performance from day one.

Methodology and instrumentation

The preparation process involves verifying four categories:

• Workspace and bench specifications: adequate height, depth, weight support, and stability.
• Environmental conditions: controlled temperature (4–40 °C), humidity (10–90% RH operating), low vibration, indoor use only.
• Utility requirements: 100–240 V AC, 50/60 Hz power, surge-protected outlets, and grounding.
• Safety equipment: emergency-stop pendant, interlocks, optional light curtain, shields, and compliance documentation.

The primary instruments are the Bravo platform itself (standard or SRT versions), optional pump module, pipette heads or tip heads (96ST, 384ST, 96LT, fixed-tip variants), and an Agilent-supplied HP mobile workstation running VWorks software.

Main details and discussion

Bench space must accommodate the Bravo footprint (~52 kg, ~70 lbs) plus accessories and computer. Tables require a level, non-warping surface with locking feet or casters, supporting total system weight without flexing. Environmental stability avoids heat sources, drafts, or vibrations that degrade pipetting precision, while indoor temperature and humidity ranges preserve system integrity.
Power outlets should be located behind the instrument with capacity for additional computer peripherals. The recommended computer features an Intel Core i7 CPU, 8 GB RAM, Windows 7 64-bit, and dual-band wireless network. Consumables such as pipette tips and labware must be stocked in advance based on experimental design.

Benefits and practical applications

• Reduces installation delays and avoids rescheduling costs.
• Ensures accurate liquid handling by maintaining stable operating conditions.
• Improves operator safety through proper interlock and emergency-stop provisions.
• Facilitates routine maintenance and access for service by respecting clearance requirements.

Future trends and potential applications

Integrating remote monitoring and diagnostics will further enhance uptime. Automated calibration routines and machine-learning-driven error detection could refine pipetting accuracy. Modular expansions, such as on-deck heating/cooling or plate reading, will widen application scopes in genomics, drug discovery, and high-throughput screening.

Conclusion

A comprehensive site preparation according to this checklist forms the foundation for dependable and efficient operation of the Agilent Bravo platform. Following these guidelines minimizes installation risks, optimizes performance, and protects personnel and investment.

References

• Agilent Technologies. Bravo Automated Liquid Handling Platform Safety and Installation Guide, Part Number G5409-90007.
• Agilent Technologies. Automation Solutions Products General Safety Guide, Part Number G5500-90015.