Waters XSelect HPLC Column
Brochures and specifications | 2012 | WatersInstrumentation
The growing complexity of sample matrices in pharmaceutical, environmental and industrial analysis demands chromatographic media with finely tuned selectivity and robust performance.
XSelect HPLC Columns combine advanced particle chemistries and ligand designs to address challenges in separation resolution, throughput and reproducibility.
This document introduces the design principles, phase technologies and performance attributes of the XSelect HPLC Column family.
Key objectives:
The XSelect portfolio relies on two core particle platforms and eight bonded phases:
XSelect phases exhibit broad selectivity differences quantified by S-values (selectivity factors) and retention characteristics across standard probe sets.
Key findings:
The XSelect column family offers:
Emerging directions include:
XSelect HPLC Columns represent a comprehensive solution for modern chromatographers, balancing diverse selectivity, operational stability and platform scalability.
By leveraging CSH and HSS particle innovations, analysts can achieve reproducible, high-throughput separations from method development through preparative isolation.
Consumables, LC columns
IndustriesManufacturerWaters
Summary
Importance of the Topic
The growing complexity of sample matrices in pharmaceutical, environmental and industrial analysis demands chromatographic media with finely tuned selectivity and robust performance.
XSelect HPLC Columns combine advanced particle chemistries and ligand designs to address challenges in separation resolution, throughput and reproducibility.
Objectives and Overview
This document introduces the design principles, phase technologies and performance attributes of the XSelect HPLC Column family.
Key objectives:
- Present the range of bonded phases built on Charged Surface Hybrid (CSH) and High Strength Silica (HSS) particles.
- Demonstrate reproducibility, scalability and selectivity across UPLC, HPLC and preparative formats.
- Illustrate practical method transfer strategies and preparative purification workflows.
Methodology and Instrumentation
The XSelect portfolio relies on two core particle platforms and eight bonded phases:
- Charged Surface Hybrid (CSH) technology: hybrid silica particles with controlled surface charge for improved peak shape under low-ionic-strength mobile phases.
- High Strength Silica (HSS) technology: 100% silica particles offering enhanced retention of polar analytes and mechanical strength for UPLC pressures.
- Ligand chemistries: C18, T3, Phenyl-Hexyl, Fluoro-Phenyl, Pentafluorophenyl (PFP) and Cyano phases providing diverse retention mechanisms.
- ACQUITY UPLC H-Class system with PDA detection.
- Conventional HPLC systems compatible with 2.5, 3.5 and 5 µm columns.
- Preparative HPLC setups for high-load isolations using OBD packing technology.
Main Results and Discussion
XSelect phases exhibit broad selectivity differences quantified by S-values (selectivity factors) and retention characteristics across standard probe sets.
Key findings:
- Reproducible α values between UPLC (1.7–1.8 µm) and HPLC (2.5–5 µm) formats facilitate seamless method transfer across platforms.
- Fluorinated phenyl and PFP phases deliver unique pi–pi and Lewis-acid interactions, enhancing separation of aromatic and basic compounds.
- HSS T3 columns maintain retention under 100% aqueous conditions, ideal for polar analytes.
- CSH OBD preparative columns demonstrate stable peak shape and high mass loading with volatile formic acid, eliminating TFA carryover issues.
Benefits and Practical Applications
The XSelect column family offers:
- High resolution and reproducibility for complex sample assays in QA/QC and research laboratories.
- Scalability from analytical to preparative separations, reducing development time and solvent consumption.
- Robust performance under a wide pH range (1–11 for CSH, 2–8 for HSS) and temperature conditions.
- Rapid method development and straightforward transfer between global sites and varied LC platforms.
Future Trends and Possibilities
Emerging directions include:
- Next-generation hybrid particles with tailored pore structures for biomolecule separations.
- Integration of machine-learning algorithms for predictive column selection and method optimization.
- Expansion of volatile mobile-phase additives compatible with mass-directed purification.
- Green chromatography: reducing solvent use through ultrafast and high-efficiency column formats.
Conclusion
XSelect HPLC Columns represent a comprehensive solution for modern chromatographers, balancing diverse selectivity, operational stability and platform scalability.
By leveraging CSH and HSS particle innovations, analysts can achieve reproducible, high-throughput separations from method development through preparative isolation.
Reference
- Waters Corporation. XSelect HPLC Columns Technical Overview, Form No. 720004178EN, 2012.
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