Defining the Optimum Parameters for Efficient Size Separations of ProteinsApplications | 2011 | Agilent TechnologiesInstrumentationGPC/SECIndustriesPharma & BiopharmaManufacturerAgilent TechnologiesKey wordsigg, ovalb, mins, iga, myo, mouse, plates, salt, mau, vendor, polyclonal, rad, plots, protein, time, after, separation, eluent, bio, study, hydrophobicity, keeley, mapp, peak, efficiency, before, interactions, lloyd, chymotrypsinogen, optimum, linda, particle, identification, stationary, separations, dependency, completing, ovalbumin, retention, effect, also, lysozyme, myoglobin, pink, evaluations, non, size, defining, aggregates, specific
Mobile Phase Optimization in SEC Method Development
Application NotePharma & BiopharmaMobile Phase Optimization in SECMethod DevelopmentAuthorRichard HurteauAgilent Technologies, Inc.,Wilmington, DE, USAAbstractAggregation of monoclonal antibody (mAb) products has an adverse effect ontheir efficacy as therapeutic agents. Size exclusion chromatography (SEC) is ahighly effective method for the detection of...
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Fast, High-Resolution Size Exclusion Chromatography of Aggregates in Biotherapeutics
Fast, High-Resolution Size ExclusionChromatography of Aggregates inBiotherapeuticsApplication NoteBiologics and BiosimilarsAuthorIntroductionAndrew CoffeyProtein aggregation is a critical quality attribute for biotherapeutic proteins becauseaggregates can have significant impact on safety, and may result in an antigenicresponse . Aggregates may also reduce the efficacy...
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Does Tween 20 Affect Monoclonal Antibody Separation?
Does Tween 20 Affect MonoclonalAntibody Separation?Application NoteBiotherapeutics & BiosimilarsAuthorsIntroductionKeeley Mapp and Phu T DuongTween 20 is commonly used during the preparation of biological samples. Typically,its concentration ranges from 0.01 to 0.1%. Tween 20 has various beneficialproperties, such as stabilizing proteins,...
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Helpful ‘Hints’ for Protein SEC Success
Helpful ‘Hints’ for ProteinSEC SuccessJean LaneApplications EngineerLC columns and consumables technical supportMay 27, 20201Outline for TalkGetting started•SEC definition, common terminology, and why use it?•Considerations for selecting an SEC column- Pain Points/Challenges for SEC- Importance of Pore Size and Particle Size...
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