Defining the Optimum Parameters for Efficient Size Separations of ProteinsApplications | 2011 | Agilent Technologies InstrumentationGPC/SECIndustriesPharma & BiopharmaManufacturerAgilent TechnologiesKey wordsigg, ovalb, mins, iga, myo, mouse, plates, salt, mau, vendor, polyclonal, rad, plots, time, protein, after, separation, bio, eluent, study, hydrophobicity, peak, mapp, keeley, efficiency, before, lloyd, interactions, optimum, chymotrypsinogen, linda, identification, particle, stationary, completing, separations, dependency, retention, effect, also, ovalbumin, lysozyme, pink, myoglobin, evaluations, size, defining, non, recovered, specific
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Does Tween 20 Affect MonoclonalAntibody Separation?Application NoteBiotherapeutics & BiosimilarsAuthorsIntroductionKeeley Mapp and Phu T DuongTween 20 is commonly used during the preparation of biological samples. Typically,its concentration ranges from 0.01 to 0.1%. Tween 20 has various beneficialproperties, such as stabilizing proteins,...
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Helpful ‘Hints’ for ProteinSEC SuccessJean LaneApplications EngineerLC columns and consumables technical supportMay 27, 20201 Outline for TalkGetting started•SEC definition, common terminology, and why use it?•Considerations for selecting an SEC column- Pain Points/Challenges for SEC- Importance of Pore Size and Particle Size...
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Agilent BiocolumnsAggregate/Fragment AnalysisApplication Compendium ContentsBackground2Getting Started3How to Guide - Size Exclusion Chromatography for BiomoleculeAnalysis - 5991-3651EN 4Featured Application NotesElevate Your mAb Aggregate Analysis - 5994-2709EN12222High-Resolution, High-Throughput Size Exclusion ChromatographyAnalysis of Monoclonal Antibodies - 5994-0828EN29Sensitive Native Mass Spectrometry of MacromoleculesUsing Standard Flow LC/MS...
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Agilent “How to” Guide forSIZE EXCLUSION CHROMATOGRAPHYFOR BIOMOLECULE ANALYSIS A GUIDE TO SUCCESSFUL SECThe chromatographic separation of biomolecules based on their size in solution is knownas size exclusion chromatography (SEC). Unlike other modes of chromatography, it relieson the absence of any...
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