Defining the Optimum Parameters for Efficient Size Separations of Proteins | LabRulez LCMS
 

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Does Tween 20 Affect Monoclonal Antibody Separation?
2017|Agilent Technologies|Applications
Does Tween 20 Affect MonoclonalAntibody Separation?Application NoteBiotherapeutics & BiosimilarsAuthorsIntroductionKeeley Mapp and Phu T DuongTween 20 is commonly used during the preparation of biological samples. Typically,its concentration ranges from 0.01 to 0.1%. Tween 20 has various beneficialproperties, such as stabilizing proteins,...
Key words
dimer, dimermonomer, monomerigg, iggfactor, factorafter, afterratio, ratioaggregations, aggregationsperformance, performanceaggs, aggsaggregate, aggregatefactors, factorsmin, minmultiple, multipleinjections, injectionstest
Helpful ‘Hints’ for Protein SEC Success
2021|Agilent Technologies|Presentations
Helpful ‘Hints’ for ProteinSEC SuccessJean LaneApplications EngineerLC columns and consumables technical supportMay 27, 20201 Outline for TalkGetting started•SEC definition, common terminology, and why use it?•Considerations for selecting an SEC column- Pain Points/Challenges for SEC- Importance of Pore Size and Particle Size...
Key words
hints, hintshelpful, helpfulsec, secsuccess, successprotein, proteinpore, poresize, sizeadvancebio, advancebiobuffer, bufferexclusion, exclusionmobile, mobilephase, phasedimer, dimercolumn, columnvolume
Agilent BiocolumnsAggregate/Fragment AnalysisApplication Compendium ContentsBackground2Getting Started3How to Guide - Size Exclusion Chromatography for BiomoleculeAnalysis - 5991-3651EN 4Featured Application NotesElevate Your mAb Aggregate Analysis - 5994-2709EN12222High-Resolution, High-Throughput Size Exclusion ChromatographyAnalysis of Monoclonal Antibodies - 5994-0828EN29Sensitive Native Mass Spectrometry of MacromoleculesUsing Standard Flow LC/MS...
Key words
return, returnsection, sectioncontents, contentsmau, mausec, secinsulin, insulinmin, minsize, sizeprotein, proteinmonomer, monomeradvancebio, advancebiotime, timeexclusion, exclusionaggregates, aggregatesaggregate
Agilent “How to” Guide forSIZE EXCLUSION CHROMATOGRAPHYFOR BIOMOLECULE ANALYSIS A GUIDE TO SUCCESSFUL SECThe chromatographic separation of biomolecules based on their size in solution is knownas size exclusion chromatography (SEC). Unlike other modes of chromatography, it relieson the absence of any...
Key words
size, sizemau, mausec, secpore, porecolumn, columnproteins, proteinsmolecular, molecularweight, weightexclusion, exclusionpolypropylene, polypropylenecolumns, columnsshould, shouldsmaller, smalleragilent, agilentsample
 

Similar PDF

Toggle
Does Tween 20 Affect Monoclonal Antibody Separation?
2017|Agilent Technologies|Applications
Does Tween 20 Affect MonoclonalAntibody Separation?Application NoteBiotherapeutics & BiosimilarsAuthorsIntroductionKeeley Mapp and Phu T DuongTween 20 is commonly used during the preparation of biological samples. Typically,its concentration ranges from 0.01 to 0.1%. Tween 20 has various beneficialproperties, such as stabilizing proteins,...
Key words
dimer, dimermonomer, monomerigg, iggfactor, factorafter, afterratio, ratioaggregations, aggregationsperformance, performanceaggs, aggsaggregate, aggregatefactors, factorsmin, minmultiple, multipleinjections, injectionstest
Helpful ‘Hints’ for Protein SEC Success
2021|Agilent Technologies|Presentations
Helpful ‘Hints’ for ProteinSEC SuccessJean LaneApplications EngineerLC columns and consumables technical supportMay 27, 20201 Outline for TalkGetting started•SEC definition, common terminology, and why use it?•Considerations for selecting an SEC column- Pain Points/Challenges for SEC- Importance of Pore Size and Particle Size...
Key words
hints, hintshelpful, helpfulsec, secsuccess, successprotein, proteinpore, poresize, sizeadvancebio, advancebiobuffer, bufferexclusion, exclusionmobile, mobilephase, phasedimer, dimercolumn, columnvolume
Agilent BiocolumnsAggregate/Fragment AnalysisApplication Compendium ContentsBackground2Getting Started3How to Guide - Size Exclusion Chromatography for BiomoleculeAnalysis - 5991-3651EN 4Featured Application NotesElevate Your mAb Aggregate Analysis - 5994-2709EN12222High-Resolution, High-Throughput Size Exclusion ChromatographyAnalysis of Monoclonal Antibodies - 5994-0828EN29Sensitive Native Mass Spectrometry of MacromoleculesUsing Standard Flow LC/MS...
Key words
return, returnsection, sectioncontents, contentsmau, mausec, secinsulin, insulinmin, minsize, sizeprotein, proteinmonomer, monomeradvancebio, advancebiotime, timeexclusion, exclusionaggregates, aggregatesaggregate
Agilent “How to” Guide forSIZE EXCLUSION CHROMATOGRAPHYFOR BIOMOLECULE ANALYSIS A GUIDE TO SUCCESSFUL SECThe chromatographic separation of biomolecules based on their size in solution is knownas size exclusion chromatography (SEC). Unlike other modes of chromatography, it relieson the absence of any...
Key words
size, sizemau, mausec, secpore, porecolumn, columnproteins, proteinsmolecular, molecularweight, weightexclusion, exclusionpolypropylene, polypropylenecolumns, columnsshould, shouldsmaller, smalleragilent, agilentsample
 

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