Defining the Optimum Parameters for Efficient Size Separations of Proteins
Applications | 2011 | Agilent TechnologiesInstrumentation
Pharma & BiopharmaManufacturer
Agilent TechnologiesKey words
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Does Tween 20 Affect Monoclonal Antibody Separation?
Does Tween 20 Affect MonoclonalAntibody Separation?Application NoteBiotherapeutics & BiosimilarsAuthorsIntroductionKeeley Mapp and Phu T DuongTween 20 is commonly used during the preparation of biological samples. Typically,its concentration ranges from 0.01 to 0.1%. Tween 20 has various beneficialproperties, such as stabilizing proteins,...
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Helpful ‘Hints’ for Protein SEC Success
Helpful ‘Hints’ for ProteinSEC SuccessJean LaneApplications EngineerLC columns and consumables technical supportMay 27, 20201Outline for TalkGetting started•SEC definition, common terminology, and why use it?•Considerations for selecting an SEC column- Pain Points/Challenges for SEC- Importance of Pore Size and Particle Size...
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SIZE EXCLUSION CHROMATOGRAPHY FOR BIOMOLECULE ANALYSIS
Agilent “How to” Guide forSIZE EXCLUSION CHROMATOGRAPHYFOR BIOMOLECULE ANALYSISA GUIDE TO SUCCESSFUL SECThe chromatographic separation of biomolecules based on their size in solution is knownas size exclusion chromatography (SEC). Unlike other modes of chromatography, it relieson the absence of any...
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Aggregate Analysis of a Recombinant L-Asparaginase from E. coli
Application NoteBiopharmaAggregate Analysis of a RecombinantL-Asparaginase from E. coliAuthorsAbstractAndrew Coffey andAndrea Angelo P. Tripodi,Agilent Technologies, Inc.Quantification of the aggregation of biotherapeutic proteins is an essential part ofensuring quality and safety. Recombinant proteins encounter a range of conditionsthat can contribute to...
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