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Recombinant SARS-CoV-2 Receptor Binding Domain: Comprehensive Top-Down Sequence Confirmation, Curation and O-Glycosylation Site Determination

 

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Next-generation MALDI top-down sequencing of protein biotherapeutics – expanding the scope of timsTOF technology Next-generation MALDI top-down sequencing (MALDI-TDS) improves top-down sequence analysis of biopharmaceutical proteins, as demonstrated in this note for adalimumab subunits, recombinant SARS-CoV-2 S-glycoprotein RBD expression products,…
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Extensive LC-Top-Down MS Sequence Confirmation of the NISTmAb Reference Material 8671 Characterization of therapeutic antibodies aims on primary sequence validation, localization of modifications such as glycan profiles and the determination of disulfide bond status of the target molecule. Introduction Typically,…
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How to Determine Glycan Profiles of Biopharmaceuticals from Peptide Mapping Data A new and sensitive approach combining BioPharma Compass, PASEF and VIP-HESI Abstract Glycosylation is a common critical quality attribute (CQA) of therapeutic proteins and needs to be characterized during…
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Protein Clipping Variant Analysis of Vedolizumab using dedicated workflows in BioPharma Compass Proteolytic degradation of biopharmaceuticals during their life cycle can pose health risks and require dedicated analysis. Abstract In this work we used enzymatically introduced antibody truncation variants as…
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