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Application Note Analysis of siRNA Duplexes at NonDenaturing UPLC Conditions Using MaxPeak Premier Column Technology Pratheeba Yogendrarajah, Willy Verluyten, Evelien Dejaegere, Leslie Napoletano, Jean-Paul Boon, Mario Hellings , Martin Gilar Analytical Development, Janssen Pharmaceutical Companies of Johnson & Johnson, Waters…
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Application Note Improved Chromatographic Analysis of Oligonucleotides with ACQUITY PREMIER Oligonucleotide BEH C18 Columns Martin Gilar, Zhimin Li, Jason MacLean Waters Corporation Abstract This application note illustrates sources of oligonucleotide loss due to adsorption on metallic column hardware (frits). Solutions,…
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Improving Chromatographic Separations Of Biopharmaceuticals With MaxPeak High Performance Surfaces (HPS) Technology Analytical Solutions Using MaxPeak High Performance Surfaces (HPS) Technology for Biopharmaceutical Therapeutics Biopharmaceutical therapeutics have consistently been the fastest growing segment within the pharmaceutical space and require advances…
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[ APPLICATION NOTEBOOK ] APPLICATION SOLUTIONS FOR OLIGONUCLEOTIDES Table of Contents Introduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4 [ HPLC & UPLC Separations ] Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8 Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11 Oligonucleotide…
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