MassHunter Walkup System - Quick Start Guide

Significance of the Topic

MassHunter Walkup System streamlines sample submission and analysis on Agilent LC and LC/MS instruments, enabling nonexpert users to run complex methods in central analytical laboratories. It automates queuing, method selection, and result delivery, supporting high-throughput demands in pharmaceutical, environmental, petrochemical, and food testing.

Objectives and Overview

This Quick Start Guide for version C.03.01 introduces core workflows: launching Walkup via OpenLAB CDS or MassHunter Data Acquisition, submitting and reprocessing samples locally and remotely, managing sample queues and events, and accessing online help and administration utilities.

Methodology and Instrumentation

Walkup features a ribbon interface, status bar, sample queue table, plate-tray diagram, and optional fraction-collection pane. Administrators configure user access, workflows, data paths, injection volumes, email reporting preferences, and run-group protocols. Users submit samples through a three-page wizard, specifying methods, priorities, and sample metadata.

Used Instrumentation

• Agilent InfinityLab 1260/1290 Infinity II LC modules: Preparative Binary Pump, Autosampler, Valve-Based Collector, Column Valve Module, Active Splitter
• Agilent MSD and MSD XT detectors
• MassHunter Data Acquisition for Q-TOF (B.08.00 SP1, B.08.01 SP1)
• MassHunter BioConfirm B.09.00
• Fraction Collector models G7159B, G7166A, G1364A–G1364F

Key Results and Discussion

Version C.03.01 adds support for OpenLAB CDS A.02.03/A.02.2, ChemStation Edition C.01.08, new LC/MS modules, enhanced fraction collection features, customizable data organization, selective email of results, remote workload monitoring, and flexible run-group options including overlaps and equilibration.

Benefits and Practical Applications

By simplifying instrument operation, Walkup reduces user training requirements and errors. Self-service sample submission and centralized administration accelerate workflows, improve data consistency, and offer automated email reporting. Fraction collection and run-group management further expand preparative and analytical capabilities.

Future Trends and Opportunities

Integration with laboratory information management systems and cloud platforms can enhance sample tracking and data sharing. Advanced workload balancing, AI-driven scheduling, and mobile interfaces may further optimize throughput and accessibility across distributed facilities.

Conclusion

Agilent MassHunter Walkup System C.03.01 delivers an intuitive, secure, and scalable solution for core analytical labs. Its broad instrument compatibility, flexible configuration, and comprehensive management tools support efficient high-throughput chromatography and LC/MS operations.