ASMS 2021: Rapid Desalting of Proteins for Direct Infusion Intact Mass Analysis using an Optimized SEC Tip | LabRulez LCMS
 

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Application NotePharma & BiopharmaSensitive Native Mass Spectrometryof Macromolecules Using StandardFlow LC/MSAuthorDavid L. WongAgilent Technologies, Inc.AbstractNative mass spectrometry can be used for a variety of protein-based applications,such as protein-protein interaction, protein-ligand binding, protein complexstructures, protein folding and antibody-drug conjugates. Most of...
Key words
native, nativeprotein, proteincounts, countsintact, intactdeconvoluted, deconvolutedmyoglobin, myoglobinamu, amumass, masscharge, chargeapomyoglobin, apomyoglobincomplexes, complexesheme, hemeholomyoglobin, holomyoglobindenatured, denaturedtetramer
ProceduresAnalysis of Intact Monoclonal Antibodies Using an M3 MicroLCwith the TripleTOF® 6600Robust and Sensitive Workflow for Qualitative and Quantitative Analysis of Biotherapeutic IgGsKhatereh Motamedchaboki, Remco van Soest, Daniel Warren and Jenny AlbaneseSCIEX, USAOverviewKey Challenges:Who Should Read This: Senior Scientists, Lab...
Key words
intact, intactmabs, mabsdesalting, desaltingptms, ptmscharacterization, characterizationantibodies, antibodiesmonoclonal, monoclonalreconstruction, reconstructionglycans, glycansquantitation, quantitationmultiplicity, multiplicitysensitivity, sensitivitybiopharmaview, biopharmaviewprotein, proteinmicrolc
Agilent-NISTmAbIntact & Subunit AnalysisUsing Reversed PhaseAgilent BioHPLC ColumnsApplication Compendium ContentsAgilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted fromNISTmAb RM 8671 batch. Quality control (QC) testing is performed usingAgilent LC-MS system. QC batch release test includes aggregate profile, chargevariants and intact mass information....
Key words
counts, countsmab, mabherceptin, herceptinintact, intactpage, pagecontents, contentsbonds, bondsback, backdeglycosylated, deglycosylatedmass, massdeconvoluted, deconvolutedides, idesamu, amubravo, bravoassaymap
Precise Characterization of IntactMonoclonal Antibodies by theAgilent 6545XT AdvanceBio LC/Q-TOFApplication NoteAuthorIntroductionDavid L. WongMonoclonal antibodies (mAbs) are a very important class of biopharmaceuticalmolecules. As a protein drug, thorough characterization of the mAb is required in eachof the manufacturing steps. Intact mAb...
Key words
glcnac, glcnacintact, intactbioconfirm, bioconfirmdeconvoluted, deconvolutedmass, massdeconvolution, deconvolutionamu, amudar, darantibodies, antibodiesmab, mabglycoforms, glycoformscounts, countsmaxent, maxententropy, entropymonoclonal
 

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Application NotePharma & BiopharmaSensitive Native Mass Spectrometryof Macromolecules Using StandardFlow LC/MSAuthorDavid L. WongAgilent Technologies, Inc.AbstractNative mass spectrometry can be used for a variety of protein-based applications,such as protein-protein interaction, protein-ligand binding, protein complexstructures, protein folding and antibody-drug conjugates. Most of...
Key words
native, nativeprotein, proteincounts, countsintact, intactdeconvoluted, deconvolutedmyoglobin, myoglobinamu, amumass, masscharge, chargeapomyoglobin, apomyoglobincomplexes, complexesheme, hemeholomyoglobin, holomyoglobindenatured, denaturedtetramer
ProceduresAnalysis of Intact Monoclonal Antibodies Using an M3 MicroLCwith the TripleTOF® 6600Robust and Sensitive Workflow for Qualitative and Quantitative Analysis of Biotherapeutic IgGsKhatereh Motamedchaboki, Remco van Soest, Daniel Warren and Jenny AlbaneseSCIEX, USAOverviewKey Challenges:Who Should Read This: Senior Scientists, Lab...
Key words
intact, intactmabs, mabsdesalting, desaltingptms, ptmscharacterization, characterizationantibodies, antibodiesmonoclonal, monoclonalreconstruction, reconstructionglycans, glycansquantitation, quantitationmultiplicity, multiplicitysensitivity, sensitivitybiopharmaview, biopharmaviewprotein, proteinmicrolc
Agilent-NISTmAbIntact & Subunit AnalysisUsing Reversed PhaseAgilent BioHPLC ColumnsApplication Compendium ContentsAgilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted fromNISTmAb RM 8671 batch. Quality control (QC) testing is performed usingAgilent LC-MS system. QC batch release test includes aggregate profile, chargevariants and intact mass information....
Key words
counts, countsmab, mabherceptin, herceptinintact, intactpage, pagecontents, contentsbonds, bondsback, backdeglycosylated, deglycosylatedmass, massdeconvoluted, deconvolutedides, idesamu, amubravo, bravoassaymap
Precise Characterization of IntactMonoclonal Antibodies by theAgilent 6545XT AdvanceBio LC/Q-TOFApplication NoteAuthorIntroductionDavid L. WongMonoclonal antibodies (mAbs) are a very important class of biopharmaceuticalmolecules. As a protein drug, thorough characterization of the mAb is required in eachof the manufacturing steps. Intact mAb...
Key words
glcnac, glcnacintact, intactbioconfirm, bioconfirmdeconvoluted, deconvolutedmass, massdeconvolution, deconvolutionamu, amudar, darantibodies, antibodiesmab, mabglycoforms, glycoformscounts, countsmaxent, maxententropy, entropymonoclonal
 

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