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In Depth Characterization of mAb Charge Variants with iCIEF Fractionation Followed by Peptide Mapping Analysis

 

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Characterization of monoclonal antibodies and antibody drug conjugates using iCIEF-MS online coupling platform Xiaoxi Zhang1, Tiemin Huang2, Tony Chen2, Tao Bo3, Min Du4. 1 Thermo Fisher Scientific, Shanghai, China, 2 Advanced Electrophoresis Solutions Ltd., Cambridge, Ontario, Canada, 3 Thermo Fisher…
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Native ion exchange chromatography directly coupled to Orbitrap mass spectrometry allows surface charge discrimination and online detection of intact proteins Aaron O. Bailey1, Guanghui Han2, Wilson Phung2, Paul Gazis1, Jennifer Sutton1, Wendy Sandoval2, Jonathan L. Josephs1 1Thermo Fisher Scientific, 355…
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CASE STUDY 73683 Deploying the multi-attribute method (MAM) across sites at Pfizer Pfizer is building the MAM lab-of-the-future Pfizer is a leading research-based biopharmaceutical company. At Pfizer, scientific principles, innovation, and the company’s global resources are applied to bring therapies…
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Biopharmaceutical Characterisation Compendium A complete toolbox of techniques, workflows and technologies for comprehensive biopharmaceutical characterisation Please click the circles to navigate INTRODUCTION AGGREGATE ANALYSIS CHARGE VARIANT ANALYSIS PEPTIDE MAPPING GLYCANS INTACT AND SUBUNIT ANALYSIS INTRODUCTION The resources collected in this…
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