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Characterization of monoclonal antibodies and antibody drug conjugates using iCIEF-MS online coupling platform

Posters | 2022 | Thermo Fisher Scientific | ASMSInstrumentation
LC/HRMS, LC/MS, LC/MS/MS, LC/Orbitrap
Industries
Pharma & Biopharma
Manufacturer
Thermo Fisher Scientific
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Size Exclusion Chromatography/ Mass Spectrometry Analysis of Antibody Drug Conjugates Using the Agilent 1260 Infinity II Bio-Inert LC Application Note Biologics & Biosimilars Author Abstract Sonja Schneider Recombinant monoclonal antibodies (mAbs) have emerged as important Agilent Technologies, Inc. therapeutic agents…
Key words
adcs, adcsdeconvoluted, deconvolutedadc, adctrastuzumab, trastuzumabdeglycosylated, deglycosylatedmass, masscounts, countsdrug, drugantibody, antibodysec, seclinker, linkerspectrum, spectrumcharge, chargedar, darconjugates
Analysis of Antibody-Drug Conjugates Using Size Exclusion Chromatography and Mass Spectrometry Application Note Biologics and Biosimilars Author Abstract Sonja Schneider Recombinant monoclonal antibodies (mAbs) have emerged as important Agilent Technologies, Inc. therapeutic agents for the treatment of different diseases. This…
Key words
trastuzumab, trastuzumabadcs, adcsadc, adcemtansine, emtansinedeconvoluted, deconvolutedcounts, countsdrug, drugdeglycosylated, deglycosylatedamu, amusec, secmass, massmabs, mabsspectrum, spectrumbuffer, bufferantibody
APPLICATION NOTE 72511 Complete characterization of a lysine-linked antibody drug conjugate by native LC/MS intact mass analysis and peptide mapping Authors Aaron O. Bailey,1 Stephane Houel,1 Kai Scheffler,2 Eugen Damoc,3 Jennifer Sutton,1 Jonathan L. Josephs1 Thermo Fisher Scientific 1 San…
Key words
chain, chainheavy, heavypeptide, peptideadc, adcmass, massintact, intactnative, nativesetting, settingmapping, mappingconjugation, conjugationdrug, drugemtansine, emtansinelinker, linkerantibody, antibodylight
In Depth Characterization of mAb Charge Variants with iCIEF Fractionation Followed by Peptide Mapping Analysis Xiaoxi Zhang1, Zhigui Zhou2,Min Sang2, Tiemin Huang3, Tony Chen3, Tao Bo4 and Min Du5 Thermo Fisher Scientific, 1 Shanghai, China; 4 Beijing, China; 5 Massachusetts,…
Key words
icief, iciefpqas, pqasceinfinite, ceinfinitepeptide, peptidemapping, mappingcharge, chargevariants, variantsimaging, imagingwcid, wcidpreparative, preparativethermo, thermodepth, depthrevolutionized, revolutionizedcharacterization, characterizationisoelectric
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