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A novel 75 cm column size gives increased resolution and better sequence coverage

Applications | 2019 | Thermo Fisher ScientificInstrumentation
Consumables, LC/HRMS, LC/MS, LC/MS/MS, LC/Orbitrap, LC columns
Industries
Proteomics
Manufacturer
Thermo Fisher Scientific
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Bottom-up proteomic profiling with μPAC HPLC columns
2021|Thermo Fisher Scientific|Others
Product spotlight | 000475 Proteomic profiling Bottom-up proteomic profiling with μPAC HPLC columns Abstract Keywords Micro pillar array column, μPAC, microfabrication, capillary LC, low-flow LC, RPLC-MS/MS, gradient separation, column performance, peak capacity, bottom-up proteomics In this product spotlight, the performance…
Key words
μpac, μpacprtc, prtcpeptide, peptidemin, minrel, relfwhm, fwhmµpac, µpaccounts, countsretention, retentionduration, durationbed, bedabundance, abundancecolumn, columnpacked, packedtime
Daniel Lopez-Ferrer1, Michael Blank1, Stephan Meding2, Aran Paulus1, Romain Huguet1, Remco Swart2, Andreas FR Huhmer1 1 Thermo Fisher Scientific, San Jose, CA, USA; 2Thermo Fisher Scientific, Germering, Germany Key Words Orbitrap Fusion Lumos, EASY-nLC 1200, peptide, protein, high resolution, accurate…
Key words
peptide, peptideidentifications, identificationsamong, amongprotein, proteinpeptides, peptidesgiven, givenproteins, proteinsnumber, numberperformance, performanceincreased, increasedprtc, prtcgradients, gradientsproteomics, proteomicsseparation, separationlumos
TECHNICAL NOTE LC-MS Vanquish Neo UHPLC system-to-system reproducibility ensures consistent and reliable results in nanoLC-MS proteomics Authors We investigated the intra- and inter-system variability for typical nanoLC-MS applications using the new Vanquish Neo UHPLC Christopher Pynn , Gisela Noack ,…
Key words
neo, neopeptide, peptidevanquish, vanquishwash, washprtc, prtcsystem, systemuhplc, uhplcnanolc, nanolcdigest, digestreproducibility, reproducibilitycytochrome, cytochromeprotein, proteinouter, outerretention, retentionduration
APPLICATION NOTE 21744 Robust LC Separation Optimized MS Acquisition Comprehensive Data Informatics A highly sensitive and robust 150 µm column to enable high-throughput proteomics Authors Xin Zhang, Aaron Gajadhar, Xuefei Sun,1 Mike Baynham3 1 Thermo Fisher Scientific, Sunnyvale, CA, USA…
Key words
proteomics, proteomicsrobustness, robustnessthermo, thermohela, helacolumn, columntime, timescientific, scientificmin, minprotein, proteinpeptide, peptidelifetime, lifetimedda, ddaprtc, prtcexactive, exactiveshotgun
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