Analysis of Catecholamines Using a Core Enhanced Technology Accucore HPLC Column

Importance of the Topic

Catecholamines are critical biochemical messengers that regulate cardiovascular function, metabolism, and neurological activity. Accurate quantification of these compounds in biological samples supports clinical diagnostics, therapeutic monitoring, and research into stress responses and neuroendocrine disorders.

Objectives and Study Overview

This study aims to demonstrate a rapid, high-efficiency HPLC method for the simultaneous analysis of seven catecholamine-related analytes using a core-enhanced Accucore HILIC column.

Methodology

Standard solutions of catechol, 5-HIAA, DOPAC, serotonin, dopamine, L-DOPA, and L-tyrosine were prepared at defined concentrations in aqueous/organic mobile phase. Samples were diluted to a final concentration of 30 µg/mL per analyte. Separation was performed under isocratic conditions with a mobile phase composition of 85:15 acetonitrile to 100 mM ammonium formate (pH 3.2).

Instrumentation

• Column: Thermo Scientific Accucore HILIC, 2.6 µm, 150 × 4.6 mm (Part No. 17526-154630)
• HPLC System: Standard Thermo Scientific binary pump and UV detector
• Column Temperature: 40 °C
• Flow Rate: 2.0 mL/min
• Injection Volume: 5.0 µL
• Detection Wavelength: 280 nm

Key Results and Discussion

All seven catecholamine-related compounds eluted between 0.74 and 3.20 minutes, achieving baseline resolution and symmetrical peak shapes. Retention time reproducibility was demonstrated over six replicate injections, with negligible variation. The core-enhanced particles delivered high efficiency at moderate backpressures (~157 bar).

Benefits and Practical Applications

• Rapid cycle time – full separation in under four minutes.
• Enhanced throughput – suitable for clinical and high-volume testing.
• Compatibility with conventional HPLC systems – no ultra-high-pressure equipment required.

Future Trends and Potential Applications

Advancements in superficially porous particle technology will further reduce analysis times and improve resolution. Integration with mass spectrometric detection could extend applicability to complex matrices and ultra-trace level quantitation. Automation and inline sample preparation will drive higher throughput in clinical and industrial laboratories.

Conclusion

The Accucore HILIC column enables fast, robust separation of multiple catecholamine-related analytes with excellent peak performance on standard HPLC platforms, making it an effective tool for clinical diagnostics and neurochemical research.