HPLC-UV Method for the Determination of Linezolid Using a Core Enhanced Technology Accucore Column

Importance of the Topic

Linezolid is a synthetic oxazolidinone antibiotic used to treat serious infections caused by Gram-positive bacteria, including resistant strains such as MRSA. Rapid and accurate quantification of linezolid is critical for pharmaceutical quality control, therapeutic drug monitoring, and ensuring regulatory compliance.

Objectives and Study Overview

This application note evaluates the Thermo Scientific Accucore RP-MS column for high-speed HPLC-UV determination of linezolid. The study focuses on method optimization, peak efficiency, reproducibility, and robustness.

Methodology

The analysis employs reversed-phase HPLC with UV detection at 251 nm. An isocratic mobile phase of water/acetonitrile (80:20, v/v) is delivered at 0.4 mL/min. The column temperature is maintained at 30 °C and a 1 µL partial loop injection is used, with the same solvent mixture as the wash.

Instrumentation Used

• Thermo Scientific Accela UHPLC system
• Accucore RP-MS column, 2.6 µm, 100 × 2.1 mm
• UV detector set at 251 nm

Primary Results and Discussion

Linezolid eluted in under three minutes with an average retention time of 2.22 min. Six replicates showed a retention time RSD of 0.2% and peak asymmetry of 1.14 (RSD 0.9%), demonstrating high precision and excellent peak shape. The core-shell particle design provided efficient separation with minimal tailing and maintained backpressure around 300 bar, lower than typical sub-2 µm columns.

Benefits and Practical Applications

• High-throughput capability for rapid quality control turnaround
• Robust peak symmetry and low tailing enhance quantitation accuracy
• Lower backpressure extends column life and permits extended operation on standard UHPLC systems

Future Trends and Opportunities

Core-shell technologies are poised for broader application to other antibiotics and small molecules, accelerating method development. Coupling with mass spectrometry and adopting miniaturized, green-solvent protocols will further improve sensitivity, throughput, and sustainability.

Conclusion

The Accucore RP-MS column delivers a rapid, reliable, and reproducible HPLC-UV method for linezolid analysis. Its core enhanced design achieves high efficiency and low backpressure, making it ideal for routine pharmaceutical assays.

Reference

• MacRitchie Eilidh. HPLC-UV Method for the Determination of Linezolid Using a Core Enhanced Technology Accucore Column. Thermo Fisher Scientific Application Note ANCCSCETLINEZ, 2011.