HPLC-UV Method for the Determination of Telmisartan Using a Core Enhanced Technology Accucore Column

Importance of the topic

Telmisartan is a widely prescribed angiotensin II receptor antagonist for the management of hypertension. Rapid, reliable quantification of telmisartan in pharmaceutical formulations and biological samples is critical for quality control, regulatory compliance and therapeutic monitoring. High-throughput analytical methods that combine speed with excellent peak shape and reproducibility help laboratories increase sample throughput and maintain consistent data quality.

Study objectives and overview

This study aimed to develop and validate a fast HPLC-UV method for the determination of telmisartan, employing Thermo Scientific’s Core Enhanced Technology on an Accucore RP-MS column. Key goals included achieving sub-2-minute analysis time, minimal peak tailing, low system backpressure, and excellent method precision.

Methodology and chromatographic conditions

The method uses a 50 × 2.1 mm Accucore RP-MS column packed with 2.6 µm solid-core particles. Chromatographic separation was performed isocratically at 25 °C with a mobile phase of 20 mM ammonium acetate and acetonitrile (70:30, v/v). The flow rate was set to 0.4 mL/min. A 1 µL partial-loop injection was carried out, and telmisartan was detected by UV absorbance at 230 nm. The solid-core design reduces pressure compared to sub-2 µm materials, while delivering sharp, symmetrical peaks.

Used Instrumentation

• Thermo Scientific Accela UHPLC system
• Accucore RP-MS column, 2.6 µm, 50 × 2.1 mm
• UV detector set at 230 nm

Main results and discussion

Under the optimized conditions, telmisartan eluted at approximately 1.15 minutes. Six replicate injections yielded a retention time RSD of 0.2% and an asymmetry factor of 1.31 with RSD of 0.7%. The chromatogram demonstrated baseline separation and minimal tailing. System backpressure remained around 150 bar, confirming that Core Enhanced Technology permits high efficiency at moderate pressures.

Benefits and practical applications

• Sub-2-minute analysis accelerates sample throughput in QC environments.
• Low backpressure extends column and instrument lifespan.
• High peak efficiency and symmetry improve quantification accuracy.
• Method simplicity and robustness support routine pharmaceutical analysis.

Future trends and potential applications

Core-shell particle technology is poised to advance UHPLC methods for other small-molecule drugs, biomarkers and complex matrices. Coupling such columns with mass spectrometry or multiwavelength detection can extend their use to trace-level quantification and impurity profiling. Ongoing developments in stationary phase chemistry promise further reductions in analysis time and enhancements in selectivity.

Conclusion

The described HPLC-UV method using a Core Enhanced Technology Accucore RP-MS column delivers rapid, reproducible and efficient determination of telmisartan. Its combination of speed, low pressure and excellent peak quality makes it an attractive choice for pharmaceutical QC and research laboratories requiring high throughput and reliable performance.