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Assessing Oxidation in IgG1 Monoclonal Antibodies and Correlating at both Intact Protein and Peptide Levels

Posters | 2020 | Thermo Fisher Scientific | ASMSInstrumentation
LC/HRMS, LC/MS, LC/MS/MS, LC/Orbitrap
Industries
Proteomics
Manufacturer
Thermo Fisher Scientific
Key words
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APPLICATION BRIEF 73555 Characterization of IgG1 monoclonal antibody (mAb) oxidation variants at intact, subunit and peptide levels Authors: Sara Carillo and Jonathan Bones NIBRT – The National Institute for Bioprocessing Research and Training, Dublin, Ireland [email protected] Tom Buchanan, Kristina Srzentic…
Key words
ipilimumab, ipilimumaboxidation, oxidationhotspots, hotspotsstressed, stressedisr, isrdtlmisr, dtlmisrmass, massnibrt, nibrtthermo, thermocontrol, controlrelative, relativescientific, scientificpinpoint, pinpointlocalization, localizationunmodified
APPLICATION NOTE 73885 Complete characterization of monoclonal antibodies under native and denaturing conditions Kristina Srzentić1, Eugen Damoc2, Kai Scheffler3, Tom Buchanan4, Krisztina Radi4, Jennifer Sutton1 Thermo Fisher Scientific, 1 Cambridge, Massachusetts, US 2 Bremen, Germany 3 Germering, Germany 4 Hemel…
Key words
intact, intacttrastuzumab, trastuzumabnative, nativehcd, hcdmass, massmab, mabthermo, thermosubunit, subunitdown, downdeconvolution, deconvolutionscientific, scientificsubunits, subunitspeptide, peptidemiddle, middleheavy
APPLICATION NOTE 73672 Accelerating monoclonal antibody peptide mapping with high acquisition speed Orbitrap-based MS Authors: Phil Widdowson, Amy Claydon, Tom Buchanan Thermo Fisher Scientific, Hemel Hempstead, UK Keywords: Peptide mapping, monoclonal antibody, mAb, post-translational modification, PTM, high throughput analysis, golimumab,…
Key words
golimumab, golimumabterminal, terminaldeamidation, deamidationglycosylation, glycosylationpeptide, peptidelysine, lysinemodification, modificationmapping, mappingdigest, digestrelative, relativeoxidation, oxidationpeptides, peptidesmodifications, modificationssmart, smartglycopeptide
Technical note | 001260 Clinical research Monitoring the intact light chain of therapeutic monoclonal antibodies in human serum using an Orbitrap Exploris 240 mass spectrometer for clinical research Authors Application benefits Yvonne E. Song, Stephanie N. Samra, • Targeted quantitation…
Key words
light, lightcamrelizumab, camrelizumabbeads, beadschain, chaingolimumab, golimumabbevacizumab, bevacizumabdaratumumab, daratumumabpembrolizumab, pembrolizumabadalimumab, adalimumabmabs, mabsintact, intactnivolumab, nivolumababundane, abundanemab, mabrituximab
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