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Refinement of HR Multi-Attribute Method from Sample Preparation to Data Analysis

 

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APPLICATION NOTE 72916 A high-resolution accurate mass multi-attribute method for critical quality attribute monitoring and new peak detection Authors Haichuan Liu1, Royston Quintyn2, John Rontree3 Thermo Fisher Scientific 1 San Jose, CA 2 West Palm Beach, FL 3 Hemel Hempstead,…
Key words
intensity, intensitynistmab, nistmabmam, mamchromeleon, chromeleonpeptide, peptidestressed, stressedprtc, prtcratio, ratiothermo, thermoretention, retentionscientific, scientificfinder, findercounts, countsbiopharma, biopharmaintegration
APPLICATION NOTE 21782 Comparison of alternative approaches to trypsin protein digestion for reproducible and efficient peptide mapping analysis of monoclonal antibodies Authors Silvia Millán-Martín, Craig Jakes, Noemi Dorival-García, Nicola McGillicuddy, Sara Carillo, Amy Farrell, Jonathan Bones National Institute for Bioprocessing…
Key words
smart, smartdigest, digestadalimumab, adalimumabdigestion, digestionmagnetic, magneticmodifications, modificationsalternative, alternativepeptide, peptiderapid, rapidmapping, mappingdeamidation, deamidationrelative, relativenistmab, nistmabinduced, inducedsample
Customer note | 001731 Biopharmaceuticals Development of a systematic approach for improving tryptic digestion, recovery and chromatographic performance for mAb analysis Addressing missed cleavages and optimizing chromatographic performance of hydrophobic peptides in peptide mapping assays Authors Application benefits Dan B.…
Key words
cleavages, cleavagesmissed, misseddigest, digestguhcl, guhcldigestion, digestionsmart, smartkingfisher, kingfisherpeptides, peptideswash, washcomb, combhydrophobic, hydrophobicpeptide, peptideagc, agcpeak, peakrow
Application note | 001123 Biopharma Consistent results for peptide mapping and monitoring across three systems of the Vanquish UHPLC platform Authors Goal Craig Jakes1,2, Silvia Millán-Martín1, Assessing consistency, reproducibility of results, and method transferability across Sara Carillo , Dennis Köhler…
Key words
vanquish, vanquishduo, duohorizon, horizonflex, flexuhplc, uhplcnistmab, nistmabbsa, bsareconditioning, reconditioningpeptide, peptidesystems, systemspeptides, peptidesdeamidation, deamidationdata, datastill, stilltandem
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