Analysis of Non-Ionic Surfactant

Significance of the Topic

Monitoring trace levels of non-ionic surfactants and metal complexes is critical in environmental analysis and water quality control. A reliable method for detecting cobalt complexes ensures compliance with regulatory standards and supports industrial quality assurance.

Objectives and Study Overview

The study aims to establish a reversed-phase HPLC method for the selective separation and quantification of the cobalt complex with 4-(2-pyridylazo)-resorcinol (Co-PAR). This approach addresses the need for rapid and sensitive detection in water quality assessment.

Used Instrumentation

• HPLC system: Prominence HPLC system
• Column: Shim-pack GIST C18, 150 mm × 4.6 mm I.D., 5 μm
• Detector: SPD-20AV with tungsten lamp, detection at 510 nm
• Column heater: 40 °C

Methodology

• Mobile phase composition: Methanol and 10 mmol/L sodium tetraborate buffer (38:62, v/v)
• Flow rate: 1.0 mL/min
• Injection volume: 10 μL

Main Results and Discussion

The chromatogram exhibits a single, well-defined peak corresponding to the Co-PAR complex at a retention time of approximately 3.5 minutes. The peak shows excellent shape and baseline separation, confirming the method’s specificity. The rapid elution profile supports high sample throughput for routine analysis.

Practical Benefits and Applications

• Rapid quantification of cobalt complexes in environmental and industrial water samples
• Enhanced laboratory efficiency through short analysis time
• Improved data reliability for regulatory compliance and quality control

Future Trends and Potential Applications

Advancements may include coupling with mass spectrometric detection for lower detection limits, implementation of greener mobile phases, and integration into automated on-line monitoring systems for continuous water quality surveillance.

Conclusion

The developed reversed-phase HPLC method provides a fast, selective, and robust means of analyzing the Co-PAR complex, meeting the demands of water quality testing and industrial monitoring. Its straightforward setup and clear chromatographic performance make it suitable for routine use.

Reference

• Application News L527 (Japan/English), Shimadzu Corporation, First Edition December 2021, ERAS-1000-0125