Biotage Extrahera (User Manual)

Importance of the Topic

Automated sample preparation is a critical enabler for modern analytical laboratories, improving throughput, precision, and reproducibility. The Biotage Extrahera system integrates multiple extraction techniques—SPE, SLE, PLD, PPT and dual‐mode Hydro DME+—with programmable filtration and positive‐pressure operations. Its automated workflows reduce manual intervention, minimize cross‐contamination risks, and support GLP software features for full traceability and audit readiness.

Objectives and Study Overview

This manual provides a detailed description of the Extrahera system architecture, safe setup, maintenance procedures, and optional GLP software functions. Key aims are to guide users through installation, method development, routine operation, instrument calibration, troubleshooting, and remote monitoring capabilities.

Methodology and Instrumentation

The Extrahera platform comprises five solvent inlets feeding disposable reservoirs, a robotic pipette head with interchangeable nozzles, a lifter/carousel assembly for sample and collection plates, and a pressure unit for gas‐driven filtrations. The optical scanner monitors solvent levels and clog detection scans assess extraction bed flow. An adjustable touch screen with optional barcode/QR reader controls solvent and sample parameters. Optional Biotage GLP software adds user access levels, e-mail notifications, audit trails, and network integration. Instrumentation used:

• Biotage Extrahera automation platform
• Biotage Disposable Pipette Tips and Solvent Reservoirs
• 96-, 48-, and 24-position extraction plates and column racks
• Pressure unit (6 bar supply) with dual flow capability
• Vacuum pump and waste collector
• 12″ adjustable touchscreen, barcode/QR scanner (optional)

Key Results and Discussion

The manual demonstrates how to configure and execute SPE, SLE, PLD, PPT, Filtration, and Hydro DME+ methods, including pressure gradients, clog‐clear routines, and end‐of‐run reporting. Users can program solvent conditioning, equilibration, sample loading in aliquots, washing, elution, and plate drying steps with precise flow rates and air gaps. GLP features enforce method locking, user accountability, and e-mail alerts for pauses or errors. Remote monitoring via a web viewer replicates on‐instrument status in real time.

Benefits and Practical Applications

Extrahera’s versatile workflows support diverse sample matrices—biological fluids, food extracts, environmental samples—and reduce variability through reproducible tip conditioning, automatic solvent refills, and precise pressure control. GLP‐enabled audit trails and network backup ensure regulatory compliance for pharma and food safety testing. Throughput is increased by unattended runs, automated clog clearance, and integration with LIMS via network shares.

Future Trends and Applications

Advances may include integration with ultra‐high‐throughput plate readers, IoT‐enabled predictive maintenance, cloud‐based method sharing, and AI‐driven method optimization. Expansion to additional sample preparation chemistries, miniaturized consumables, and real‐time process analytics will further streamline workflows in clinical, environmental, and industrial QC laboratories.

Conclusion

The Biotage Extrahera system offers a flexible, automated solution for complex sample preparation tasks. Its modular hardware, programmable liquid‐handling, gas‐driven extraction, and comprehensive GLP software features address productivity, traceability, and compliance requirements. This manual equips users with the knowledge to deploy, operate, maintain, and troubleshoot Extrahera in routine and regulated environments.

References

1. Biotage Extrahera User Manual, Document P/N 414158-M, Biotage AB, 2020
2. ISO 8573-1:2010 Compressed Air–Particulate, Water, and Oil–Purity Classes
3. Biotage Application Notes on SLE, SPE, PLD, PPT, and Hydro DME+ techniques