Operating the Biotage Horizon 5000 Software

Significance of the topic

The efficient and reliable control of solid-phase extraction (SPE) systems is critical in modern analytical laboratories. User-friendly software that balances routine operation with method development enhances throughput, reproducibility and compliance with regulatory protocols.

Objectives and overview

This document presents the functionality of the Biotage Horizon 5000 Software (formerly SPE-DEX 5000), focusing on two primary workflows: simple execution of stored methods for routine analysts and flexible method creation or modification by administrators.

Instrumentation used

The software operates the Biotage SPE-DEX 5000 sample preparation system, managing up to three parallel SPE stations and associated solvent delivery, sensors and disk holders.

Methodology and instrumentation

Routine users access a streamlined interface protected by basic passwords. Methods such as “Prime Sample Lines” are selected via pull-down menus, assigned to stations, and sample identifiers entered via keyboard or barcode scanner. The software guides the user through hardware checks before initiating runs. Administrators unlock advanced features to build or edit methods step-by-step. Pull-down lists allow selection of solvent types and volumes, supporting protocols like US EPA 525.2 for drinking water analytes. Real-time process monitoring and status screens display progress for each station.

Main results and discussion

Operational testing demonstrates that routine users can start runs in only a few clicks, minimizing training time and errors. Administrator mode enables precise configuration of multi-step protocols, ensuring regulatory compliance. The system completes runs unattended once initiated, while built-in pause and alert functions notify users of issues such as disk overflows or flow interruptions, allowing rapid intervention.

Benefits and practical applications

• Streamlined routine workflows reduce operator errors and training requirements.
• Customizable method development supports regulatory and in-house protocols.
• Real-time alerts and pause features safeguard sample integrity and operator safety.
• Unattended operation increases lab efficiency and throughput.

Future trends and applications

Integration with laboratory information management systems (LIMS) and cloud-based data platforms could further streamline data handling. Incorporating advanced analytics or AI-driven method optimization may enhance extraction efficiency. Expansion of method libraries for challenging matrices and connectivity with remote monitoring tools represent promising developments.

Conclusion

The Biotage Horizon 5000 Software delivers a robust solution for SPE automation, combining simplicity for routine users with deep configurability for method developers. Its comprehensive monitoring, alerting and flexible method architecture make it well suited for diverse applications in environmental, pharmaceutical and quality-control laboratories.