Installation Instruction for ProfIC Vario 1 Amperometry

Význam tématu

The ProfIC Vario 1 Amperometry system combines ion chromatography with electrochemical detection to enable fully automated, reliable analysis of electroactive compounds. It addresses routine laboratory needs in environmental monitoring, food analysis, and quality control by offering high sensitivity, reproducibility, and ease of use.

Cíle a přehled studie / článku

This application bulletin provides step-by-step installation and configuration instructions for the ProfIC Vario 1 Amperometry system. It covers hardware setup, software installation, device interconnections, method adjustment in MagIC Net, system purging, and a demonstration of sugar analysis in water and apple juice.

Použitá metodika a instrumentace

The system comprises:

• 940 Professional IC Vario ONE with amperometric detector (Au/Pd Wall-Jet cell)
• 858 Professional Sample Processor with swing-head autosampler
• MagIC Net 3.0 software for instrument control and data evaluation
• Metrosep Carb 2 – 150/4.0 analytical column with guard column
• Eluent: 100 mmol/L NaOH + 10 mmol/L sodium acetate in ultrapure water
• Optional Liquid Handling Station for automated rinsing and dilution

Key steps include software installation, device naming in MagIC Net, defining rack positions and amperometric cell parameters, importing and customizing methods, and purging to remove air bubbles.

Hlavní výsledky a diskuse

An exemplary measurement demonstrates separation and quantification of sugar alcohols and sugars (inositol, arabitol, sorbitol, glucose, xylose, fructose, lactose, sucrose, maltose) in standards and diluted apple juice. Multipoint calibration over 0.25–60 mg/L yielded excellent linearity (r² ≥ 0.9999) and low relative standard deviations (<1.4 %). These results confirm the system’s precision and robustness for routine carbohydrate analysis.

Přínosy a praktické využití metody

The ProfIC Vario 1 Amperometry workflow offers:

• Automated sample handling and low carry-over via counter-current flow
• High sensitivity for electroactive analytes using pulsed amperometric detection
• Flexible method adjustment within MagIC Net software
• Scalable throughput for research, QA/QC, and industrial laboratories

Budoucí trendy a možnosti využití

Future developments may include integration of online sample preparation (e.g., Liquid Handling Station), expanded applications for trace analysis of pharmaceuticals and environmental contaminants, and advanced data analytics with AI-driven peak identification.

Závěr

This application bulletin delivers a comprehensive guide for installing and configuring the ProfIC Vario 1 Amperometry system, ensuring reliable chromatographic separation and amperometric detection of electroactive compounds. The demonstrated performance underscores its suitability for diverse analytical challenges.

Reference

• Application Bulletin 364: Installation Instruction for ProfIC Vario 1 Amperometry, Metrohm