Installation Instruction for ProfIC Vario 1 PCR-UV/VIS

Importance of Topic

Ion chromatography with post-column reaction UV/VIS detection delivers automated, sensitive analysis of UV/VIS-active analytes, crucial for routine quality control, environmental monitoring, and research laboratories.

Objectives and Overview

This bulletin provides step-by-step instructions for installing and configuring the ProfIC Vario 1 PCR-UV/VIS system, including hardware assembly, software setup in MagIC Net, and method initialization.

Methodology and Instrumentation

• Software Installation and Configuration
  • Install MagIC Net via CD, accept default directories, restart Windows.
  • Connect instruments via USB, assign names and device roles in configuration.
• Hardware Assembly
  • Unpack IC module, reactor, detector, sample processor, eluent module, and accessories.
  • Mount reactor and detector on or beneath IC tower, secure bottle holders, remove transport locks, connect leak sensor and drainage tubing.
  • Attach sample processor: connect controller and power cables, install peristaltic pump tubing and capillaries, position swing head, install rack.
  • Capillary Routing
    1. Eluent inlet to pump.
    2. Pump outlet to column inlet (0.25 mm ID).
    3. Column outlet to reactor Y-connector.
    4. PCR reagent via peristaltic pump to reactor second Y-port.
    5. Reactor outlet to detector inlet; detector outlet to waste collector.
  Instrumentation
  • 940 Professional IC Vario 1
  • 943 Professional Reactor Vario
  • 944 Professional UV/VIS Detector Vario
  • 858 Professional Sample Processor
  • Optional: High-pressure pump (HPG), Dosino, Liquid Handling Station (LQH), Eluent Production Module (941)
  
  

  Key Results and Discussion

  
  After equilibration and method import, chromate (CrVI) calibration shows linear response (R²≈0.9999), reproducible detection down to 0.05 µg/L, and stable baselines. Comparison of PCR delivery methods demonstrates that high-pressure pumping yields lowest noise and best long-term stability, while Dosino offers automated rinsing at the expense of refill delays.
  

  Benefits and Practical Applications

  
  
  • Fully automated sampling and derivatization reduce manual workload and contamination risk.
  • High sensitivity and reproducibility support trace analysis in water quality, industrial process control, and pharmaceutical QA/QC.
  • Modular configuration allows tailored reagent delivery and column selection.
  
  

  Future Trends and Possible Applications

  
  
  • Integration with advanced robotics and LIMS for high-throughput workflows.
  • Miniaturized microreactor designs for lower reagent consumption.
  • Implementation of real-time monitoring and AI-driven method optimization.
  
  

  Conclusion

  
  The ProfIC Vario 1 PCR-UV/VIS system offers a robust, versatile platform for automated post-column derivatization and UV/VIS detection, delivering reliable, high-sensitivity analyses adaptable to diverse laboratory needs.
  

  Reference

  
  
  • Application Bulletin 363: Installation Instruction for ProfIC Vario 1 PCR-UV/VIS