Sampling the human population – ultra-high- throughput plasma protein profiling (uHTPPP) sample preparation for translational proteomics
Applications | 2020 | Thermo Fisher ScientificInstrumentation
LC/HRMS, LC/MS, LC/MS/MS, LC/Orbitrap
IndustriesProteomics , Clinical Research
ManufacturerThermo Fisher Scientific
Key wordsuhtppp, peptide, serum, hamilton, easypep, workflow, plate, recovery, script, spin, thermo, plasma, pipetting, sample, lysis, pooled, scientific, preparation, protocol, patient, digestion, kit, automated, normal, protein, from, efficiency, starlet, fluorometric, automation, solution, peptides, wash, using, user, highthroughput, deck, manual, lung, samples, alkylation, microlab, layout, compared, averaged, fluorescent, pierce, cysteine, handling, dilution
Similar PDF
An Ultra High-throughput Plasma Protein Profiling (uHTPPP) Workflow Using a Modified Quadrupole-Orbitrap Mass Spectrometer
2020|Thermo Fisher Scientific|Posters
An Ultra High-throughput Plasma Protein Profiling (uHTPPP) Workflow Using a Modified Quadrupole-Orbitrap Mass Spectrometer Michelle L. Dubuke1, Sarah Trusiak1, Sitara Chauhan1, Ryan D. Bomgarden2, Sergei Snovida2, Bhavin Patel2, Emily I. Chen1. 1Thermo Fisher Scientific, Precision Medicine Science Center, Cambridge, MA.…
Key words
serum, serumgroups, groupsdepleted, depletedhamilton, hamiltonpmsc, pmscprotein, proteinspin, spinproteins, proteinsidentifications, identificationspeptide, peptideplasma, plasmaquantified, quantifiedhela, helaplate, platerecovery
High-throughput plasma proteomics: A standardized and scalable workflow for quantitative protein profiling in large sample cohorts
2019|Thermo Fisher Scientific|Applications
APPLICATION NOTE No. 73194 High-throughput plasma proteomics: A standardized and scalable workflow for quantitative protein profiling in large sample cohorts Keywords Q Exactive, Orbitrap, protein biomarker, plasma workflow, serum workflow, Evosep, high throughput proteomics workflow Authors Jing Wang1; Sarah Trusiak1;…
Key words
cancer, cancerhealthy, healthylung, lungplasma, plasmascaled, scaledevosep, evosepeasypep, easypepabundance, abundanceworkflow, workflowprotein, proteinpeptide, peptidegroups, groupsalt, altpatient, patientthroughput
Orbitrap Exploris 240 mass spectrometer delivers significantly increased lab productivity and reproducibility of human plasma proteome profiling from large human cohort studies
2021|Thermo Fisher Scientific|Others
CASE STUDY 66002 Orbitrap Exploris 240 mass spectrometer delivers significantly increased lab productivity and reproducibility of human plasma proteome profiling from large human cohort studies “For large clinical cohort studies where hundreds of plasma samples are to be analyzed, a…
Key words
plasma, plasmauncg, uncggreensboro, greensborocarolina, carolinapmsc, pmscuhtppp, uhtppphundreds, hundredscohort, cohorthuman, humansamples, samplesprofiling, profilingbiomedical, biomedicalreproducibility, reproducibilitylaboratory, laboratoryworkflow
Thermo Scientific™ Orbitrap Exploris™ 240 mass spectrometer - Plasma profiling–reliable large cohort analysis with a fully automated workflow
2020|Thermo Fisher Scientific|Technical notes
Proof of performance Orbitrap Exploris 240 mass spectrometer Plasma profiling–reliable large cohort analysis with a fully automated workflow Summary Here we present an Ultra-High Throughout Plasma Protein Profiling (uHTPPP) workflow (Figure 1), from automated sample preparation to high-resolution, accurate-mass (HRAM)…
Key words
evosep, evosepexploris, explorisorbitrap, orbitrapone, oneuhtppp, uhtpppworkflow, workflowpeptide, peptidecohort, cohortsample, sampleautomated, automatedprotien, protienstarlet, starletevotip, evotipprep, prepdipvpkpk