Konference CEEPC/IPM/CMSC 2022 - Sborník abstraktů

Importance of the topic

Analytical proteomics and mass spectrometry are pivotal disciplines for exploring the molecular underpinnings of biology, medicine and biotechnology. The 16th Central and Eastern European Proteomic Conference together with the 10th Czech Mass Spectrometry Conference showcased cutting-edge advances in quantitative proteomics, single-cell analysis, native mass spectrometry, multi-omics integration and novel instrument developments. Such methods are essential for biomarker discovery, drug development, structural biology, and precision medicine.

Objectives and overview of the conference

The meeting aimed to present the latest innovations in sample preparation, data acquisition and data analysis, bridging method development and applications in biomedicine, environmental research and biopharma. Over three days, plenary lectures, oral sessions and poster presentations covered topics from single-cell proteomics to large-scale metabolomics and immunoassays, with emphasis on high sensitivity, throughput, and novel fragmentation techniques.

Methodology and instrumentation used

Key methodologies included single-cell sample preparation with nanoliter volumes (cellenONE® / proteoCHIP), data-independent acquisition (dia-PASEF) on TIMS-TOF instruments, high-resolution ICR and Orbitrap MS for global metabolomics, cyclic ion mobility mass spectrometry for native complexes, and MALDI-TOF for rapid species or biomarker screening. Techniques such as HDX-MS for antibody structure, HILIC and RPLC for glycopeptide separation, and targeted SRM/SRM-MS panels for clinical markers were presented. The novel ZenoTOF 7600 QTOF demonstrated duty-cycle enhanced MS/MS and electron-activated dissociation.

Main results and discussion

Highlights included identification of >1,500 proteins per single cell without carriers, metabolic fingerprinting of Alzheimer’s disease models via ICR-MS, LUX-MS for mapping surface receptor nanoscale organization, and discovering novel cryoprotectant mixtures in freeze-tolerant insects. Proteogenomic tear fluid profiling and high-throughput µLC-MS lipidomics of 3D cellular models illustrated clinical and drug-development applications. Optimized sample prep workflows for membrane proteins, antibodies and intact proteins were shown to yield high coverage and quantitation reproducibility.

Benefits and practical applications

These advances enhance biomarker discovery in cancer, neurodegeneration and cardiovascular disease, improve quality control in biopharmaceuticals (antibodies, peptide therapeutics), and enable rapid pathogen or species identification. Native MS and ion mobility expand structural biology capabilities, while label-free and immuno-PCR hybrid immunoassays offer new clinical diagnostic tools.

Future trends and opportunities

Integration of multi-omics data (proteomics, metabolomics, glycomics) with AI and advanced bioinformatics will unlock complex disease mechanisms. Emerging methods in single-cell proteomics, cyclic ion mobility fragmentation, and real-time sampling (ASAP-MS) promise deeper insights at high throughput. Further miniaturization, automation and standardization will drive broader adoption in clinical laboratories and environmental monitoring.

Conclusion

The conference underscored a vibrant proteomics and mass spectrometry community delivering innovations from fundamental biophysics to translational research. Continued collaboration across disciplines and technology providers will accelerate discoveries and applications in health, biotechnology and environmental sciences.