BOOK OF ABSTRACTS: THIRTEENTH ANNUAL CONFERENCE OF THE CZECH SOCIETY FOR MASS SPECTROMETRY together with ELEVENTH INFORMAL PROTEOMIC MEETING

Importance of the Topic

The rapid evolution of mass spectrometry and proteomics drives breakthroughs in life sciences, enabling deep characterization of proteins, metabolites, and environmental chemicals. These tools support precision health, biomarker discovery, biopharmaceutical quality control, and exposome research across disciplines.

Conference Overview and Objectives

The 13th Annual Conference of the Czech Society for Mass Spectrometry, held in České Budějovice (November 19–21, 2025), united academic and industry experts to present advances in instrumentation, workflows, and applications. Sessions spanned fundamental MS developments, proteomics, metabolomics, structural MS, and exposomics, fostering collaboration and knowledge exchange.

Methodologies and Instrumentation

Highlights included novel analyzers (Orbitrap Astral, timsTOF SCP), fast photochemical labeling (FPOP), native and HDX-MS, advanced LC–MS/MS (nano, micro, high-flow), GC×GC-HRMS, MALDI imaging, and automated sample prep for large-scale exposome mapping. Artificial intelligence and ion‐mobility integration emerged as key enablers.

Main Outcomes and Discussions

• Orbitrap Astral demonstrated sub-nanogram sensitivity for single-cell proteomics
• Proteogenomic profiling refined triple-negative breast cancer subtypes and targets
• Automated liquid–liquid extraction with GC-Orbitrap enabled cohort-scale exposome screening
• HILIC-FLD-MS advanced simultaneous peptide and glycan QA of biopharmaceuticals
• Microflow LC–MS optimized via design of experiments doubled peptide identification
• MALDI and DESI imaging mapped hormones, metabolites, and TF binding in situ

Practical Applications and Benefits

The showcased methods improve throughput, sensitivity, and quantitative accuracy for clinical proteomics, environmental monitoring, biomarker discovery, and biopharmaceutical QC, reducing sample requirements and accelerating workflows.

Future Trends and Potential Uses

Next-generation trends include AI/ML-driven data analysis, integrated multi-omics, single-cell deep profiling, 4D metabolomics/imaging, expanded exposome networks, and novel fragmentation strategies to decode complex proteoforms.

Conclusions

This joint meeting underscored the vibrant progress and cross-disciplinary impact of mass spectrometry. Continued innovation in instrumentation, workflows, and informatics will sustain rapid advances across pharmaceutical, clinical, environmental, and fundamental research.