Using Agilent Fraction Collectors in Thermo Scientific Chromeleon with ICF Integration

Significance of the topic

Fraction collection is a key element in preparative liquid chromatography workflows, enabling targeted isolation of analytes for further analysis or use. Integrating Agilent fraction collectors into Thermo Scientific Chromeleon via ICF delivers enhanced automation, but poses specific requirements and limitations. Understanding these details is vital for routine laboratory applications to ensure reliable, contamination-free fractionation.

Objectives and overview

This technical note aims to describe how to configure and operate Agilent fraction collectors within Chromeleon using ICF integration. It outlines recommended system architecture, module compatibility, method setup, reporting capabilities, and cluster configurations. Key goals include identifying best practices, highlighting current limitations, and preparing users for upcoming driver enhancements.

Used methodology and instrumentation

Chromatography system requirements and integration approach:

• Agilent-only LC systems connected via CAN bus to leverage built-in intelligence among Agilent modules.
• ICF (Instrument Control Framework) integration is currently the sole supported method for fraction collection in Chromeleon.
• Supported fraction collector modules: G1364A/B/C/D, G1364E/F, G5664A, G7166A, G7158B, G7159B, and recovery collectors (G9322A) in clusters.
• Chromeleon ICF version 2.6 with LC Driver A.02.19 SR2 or higher; driver features above 3.0 require later software updates.

Main results and discussion

Key findings and operational specifics:

• Stoptime definition is mandatory for fraction collectors to synchronize runtime and enable internal calculations for time-based fractionation.
• Start locations must be assigned via the Home ePanel (Advanced tab) using absolute addressing (e.g., P1-B-1) or linear vial numbering; logical offsets and built-in pooling are not supported.
• Overfill handling is rudimentary: when a container’s capacity is exceeded, the system continues to the next location without aborting, risking cross-contamination.
• Manual fraction triggers are logged in the audit trail as informational entries; graphical chromatogram overlays and built-in Tube reports assist in post-run review, though trigger reasons (signal, manual, overfill) are not explicitly reported.
• Clusters support up to three main collectors with a recovery module; configuration requires careful naming (alphanumeric and underscores only) and manual entry of min/max sensor values.

Benefits and practical applications

The described setup suits routine fraction collection tasks where the number of fractions is predetermined. Benefits include:

• Automated, on-the-fly configuration changes through Chromeleon dashboard.
• Seamless application of driver settings without halting runs.
• Integration of fraction preview via reference chromatograms (CDS ICF 3.0 and above).
• Ability to cluster modules for high-throughput preparative workflows.

Future trends and possibilities

Upcoming developments will enhance user experience and expand capabilities:

• Agilent Drivers for Chromeleon are slated to natively support fraction collection, superseding ICF integration.
• LC Driver 3.x series enables advanced clustering, autoscaling between analytical and preparative modes, and flow-gradient support during fractionation.
• Potential for built-in pooling logic, dynamic start-location tracking, and robust overfill protection.

Conclusion

Integrating Agilent fraction collectors into Chromeleon via ICF provides a viable solution for controlled, automated fractionation in routine labs. While current limitations—such as basic overfill handling and lack of pooling logic—must be managed by users, forthcoming driver updates promise to streamline workflows and deliver advanced functionality.