Orbitrap Ascend Tribrid mass spectrometer - Product specifications

Thermo Scientific Orbitrap Ascend Tribrid Mass Spectrometer

Significance of the Topic

The ability to analyze complex biological and chemical samples with high sensitivity, resolution, and throughput is essential in proteomics, structural biology, and small molecule research. The Orbitrap Ascend Tribrid system integrates advanced hardware and intelligent controls to extend depth and accuracy in mass spectrometry analyses.

Objectives and Overview of the Instrument

This platform is engineered to deliver unmatched performance for full-proteome quantitation, complex peptide and protein characterization, small molecule profiling, and native protein complex analysis. Key design goals include improved labile compound capture, high-speed MSn acquisition, and flexible method development.

Methodology

The instrument employs a Tribrid architecture combining a quadrupole mass filter, dual-pressure linear ion trap, and ultra-high-field Orbitrap analyzer. Active Ion Management (AIM+) and dual Ion Routing Multipoles optimize ion transmission. Multiple fragmentation techniques (CID, HCD, ETD, EThcD, ETciD, UVPD) can be applied at any MSn stage. Intelligent features—such as real-time database and library search, Dynamic Maximum Injection Time, and Synchronous Precursor Selection—enhance acquisition speed, specificity, and quantitation accuracy.

Applied Instrumentation

• Ion sources: Ion Max NG for ESI and APCI, Auto-Ready for hands-free calibration
• Front and back Ion Routing Multipoles with variable pressure control and optional High Mass Range MSn (HMRn+)
• QR5 segmented quadrupole mass filter with hyperbolic surfaces for efficient precursor isolation
• Dual-pressure linear ion trap and next-generation ultra-high-field Orbitrap analyzers
• Optional modules: EASY-ETD/PTCR ion sources, EASY-IC calibrant source, UVPD laser, FAIMS Pro Duo interface
• Software: Xcalibur control, Proteome Discoverer, ProSight, Compound Discoverer, AcquireX, SureQuant workflows

Main Results and Discussion

Benchmark tests show up to 480,000 FWHM resolution at m/z 200, standard mass range m/z 40–2000 and extended range to m/z 16,000 with HMRn+. Acquisition rates reach 45 Hz (Orbitrap) and 50 Hz (ion trap). Mass accuracy is <1 ppm with internal calibration. Sensitivity tests demonstrate signal-to-noise >200 for femtogram-level analytes. Advanced workflows—TurboTMT, Real-Time Search, and dynamic scan management—enable high-throughput, high-precision quantitation in TMT experiments and targeted proteomics.

Benefits and Practical Applications

• Deep, quantitative proteomics with TMT labeling and multiplexed analyses
• Structural elucidation of intact proteins and native complexes
• Automated small molecule discovery and qualification workflows
• Flexible fragmentation strategies for comprehensive peptide mapping and PTM analysis
• Unattended calibration and remote diagnostics to maximize uptime

Future Trends and Opportunities

Emerging developments may include AI-driven adaptive acquisition, expanded ion mobility integration (e.g., FAIMS), deeper top-down proteomics coupling, real-time method optimization, and cloud-based remote monitoring. Extended mass range capabilities and advanced software analytics will further empower discovery in complex biological systems.

Conclusion

The Orbitrap Ascend Tribrid mass spectrometer offers a versatile, high-performance solution for the most demanding analytical challenges in proteomics, structural biology, and small molecule research. Its combination of leading-edge hardware, intelligent software, and modular options delivers the sensitivity, speed, and flexibility required for current and future mass spectrometry workflows.