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Identification of a Hydroxylysine-Containing Peptide Using AAA-Direct

 

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Technical Note 50 Determination of the Amino Acid Content of Peptides by AAA-Direct INTRODUCTION EQUIPMENT ™ The AAA-Direct system separates amino acids on a high performance anion-exchange column and directly detects them by integrated amperometry.1, 2 AAA-Direct has advantages over…
Key words
amino, aminohydrolysis, hydrolysisaaa, aaaacid, acidmsh, mshacids, acidstrp, trpreplicate, replicateblanks, blankspeptide, peptidetyr, tyrarg, argser, sergly, glydirect
Application Note 150 Determination of Amino Acids in Cell Cultures and Fermentation Broths INTRODUCTION Cell cultures and fermentation broths are used in the manufacture of biotherapeutics and many other biological materials produced using recombinant genetic technology. Broths are also used…
Key words
amino, aminobroths, brothsfermentation, fermentationacids, acidsbroth, brothsigma, sigmanaoh, naohcarbohydrates, carbohydratescultures, culturesaaa, aaaypd, ypdmedia, mediacell, cellchemical, chemicalculture
Determination of Protein Concentrations Using AAA-Direct
2016|Thermo Fisher Scientific|Applications
Application Note 163 Determination of Protein Concentrations Using AAA-Direct™ INTRODUCTION Dye-binding assays such as bicinchoninic acid (BCA), Lowry, and Bradford (Coomassie) are the most common methods to determine total protein. The BCA assay depends on Cu (II) reduction to Cu…
Key words
aaa, aaaprotein, proteinamino, aminohydrolysate, hydrolysatebsa, bsadirect, directundiluted, undilutedconcentrations, concentrationsacid, acidconcentration, concentrationwater, wateracids, acidshydrolysis, hydrolysistotal, totalusing
Determination of Tryptophan Using AAA-Direct
2001|Thermo Fisher Scientific|Applications
Application Note 142 Determination of Tryptophan Using AAA-Direct ™ INTRODUCTION Tryptophan (Trp) is a difficult amino acid to determine in proteins and peptides because it chemically decomposes during acid hydrolysis. Consequently, many procedures have been developed for the independent preservation…
Key words
trp, trptryptophan, tryptophanaaa, aaahydrolysis, hydrolysisamino, aminopeptide, peptideprotein, proteinhydrolysates, hydrolysatesbsa, bsadirect, directdetermination, determinationrecovery, recoveryusing, usingculture, cultureacids
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