Agilent InfinityLab 96-Well Plate, 0.33 mL, Round Wells, V Shape, Polypropylene, 14 mm

Significance of 96-Well Polypropylene Storage Plates

In modern analytical chemistry workflows, reliable sample storage and handling are critical for reproducible results in chromatography, LC–MS, and high-throughput screening. The design of a well plate can directly impact sample recovery, chemical compatibility, and automation efficiency, making the selection of an appropriate microplate essential for research, quality control, and clinical laboratories.

Objectives and Overview

This datasheet presents the Agilent InfinityLab 96-well plate with 0.33 mL round, V-shaped wells made from medical-grade polypropylene. The primary goal is to deliver a universal plate for sample collection, storage, precipitation, and centrifugation that is compatible with common autosamplers and sealing mats.

Methodology and Materials

The plate is manufactured from virgin polypropylene to ensure low biomolecular binding, broad chemical resistance (including DMSO, phenol, chloroform), and tolerance to temperatures ranging from –80 °C to 120 °C. The V-shaped bottom facilitates complete retrieval of small volumes, while the flat, clear top surface ensures uniform sealing with silicone mats. The footprint adheres to SBS standards for stackability and compatibility with automation platforms.

Main Technical Specifications

• Well count: 96 round wells
• Nominal volume: 330 µL; working volume: 300 µL
• Well diameter: 8.5 mm; depth: 11 mm
• Plate dimensions: 127.8 mm length × 85.5 mm width × 14.4 mm height
• Grid: alphanumeric numbering; skirted frame
• Material properties: autoclavable, RNase/DNase-free, nonsterile, clear polypropylene
• Temperature range: –80 to 120 °C
• Stackable SBS footprint; compatible with sealing mat (p/n 5042-1389)
• Pack sizes available: 25, 50, or 100 plates

Key Benefits and Discussion

The V-shaped wells maximize recovery of low-volume samples, reducing carryover and improving quantitation in sensitive assays. Low binding surfaces preserve sample integrity for proteomics and genomics workflows. The plate’s broad chemical resistance and temperature tolerance expand its use from cryogenic storage to high-temperature incubations. Standardized SBS dimensions and compatibility with sealing mats and autosamplers streamline integration into high-throughput and automated laboratories.

Practical Applications

• Chromatography sample preparation and fraction collection
• High-throughput screening in drug discovery
• Biomolecule storage for proteomic or genomic analysis
• Centrifugation-based precipitations and pelleting
• Automated liquid handling and robotic workflows

Future Trends and Potential Uses

Advances in microplate materials may further reduce nonspecific binding and enhance chemical compatibility. Integration with smart lab systems could allow real-time tracking of plate usage and environmental conditions. Development of next-generation plates with embedded barcodes or RFID tags will support sample traceability in regulated environments. Additionally, adoption of ultra-low dead-volume formats will address the growing demand for miniaturized assays in personalized medicine and single-cell analysis.

Conclusion

The Agilent InfinityLab 96-well polypropylene plate offers a robust solution for diverse analytical workflows, combining efficient sample recovery, chemical resilience, and automation compatibility. Its design supports reproducibility and throughput in research and industrial laboratories, making it a versatile tool for modern analytical chemistry.

Reference

Agilent InfinityLab 96-Well Plate, 0.33 mL, Round Wells, V Shape, Polypropylene, 14 mm (Agilent Technologies, 2023)