Sample Position Identification and Measurement Confirmation

Significance of the topic

High‐throughput analytical laboratories require robust sample identification and tracking to ensure data integrity, reduce human error, and accelerate workflows. Integrating barcode reading directly into an autosampler streamlines sample loading, minimizes manual inputs, and enhances traceability from vial to final report.

Objectives and study overview

This technical overview demonstrates how the Agilent 1290 Infinity III Multisampler equipped with the InfinityLab Sample ID Reader automatically recognizes vial barcodes and assigns sample positions without manual entry. The study uses parabens as model analytes to confirm identity and position, illustrating improved ease of use and reduced error rates.

Methodology and instrumentation

The workflow combines the following components and steps:

• Instruments: Agilent 1290 Infinity III pump, Multisampler with internal Sample ID Reader, MCT, diode array detector, and handheld USB barcode scanner.
• Software: Agilent OpenLab CDS (v2.8+) configured to read barcodes before injection.
• Column and solvents: ZORBAX Eclipse Plus C18 RRHD, water and acetonitrile gradient.
• Sample preparation: Methylparaben, ethylparaben, propylparaben, butylparaben dissolved at 100 mg/L in acetonitrile, each in vials bearing bottom QR‐code labels.
• Procedure: Barcodes scanned into the expected barcode field in the sequence table. Vial trays (40 positions) loaded randomly; the built‐in reader scans entire trays to identify sequence samples and assign drawer and position.

Main results and discussion

Randomly positioned paraben samples were successfully identified and linked to acquisition data. Corner and central tray locations, as well as front and back positions across two drawers, were detected consistently. Chromatograms overlaid by sample show correct annotation of drawer and position (e.g., D1F‐D1 for drawer 1, front position 1). The system confirmed expected barcodes post‐run in OpenLab Data Analysis, demonstrating reliable end‐to‐end sample tracking.

Benefits and practical applications

• Time savings by eliminating manual position entry.
• Reduced risk of sample mix‐ups and transcription errors.
• Enhanced regulatory compliance and audit readiness through automated tracking.
• Seamless integration into existing HPLC workflows.

Future trends and potential applications

Advances may include integration with laboratory information management systems (LIMS), use of RFID tags alongside barcodes, AI‐driven sample scheduling and method optimization, and expansion to other chromatographic and mass spectrometric platforms for fully automated, error‐proof workflows.

Conclusion

The Agilent 1290 Infinity III Multisampler with built‐in Sample ID Reader offers a streamlined, reliable solution for automatic sample position identification. By reading vial barcodes directly from the tray, the system minimizes manual input, reduces errors, and supports a fully traceable analytical workflow.

References

1. Confirmation of Sample Position – Using the Agilent 1290 Infinity III Multisampler with Agilent InfinityLab Sample ID Reader – Part 1 of 2. Agilent Technologies technical overview, publication 5994-7568EN, 2024.
2. Agilent Advanced Sample Linking – A Complete Workflow from Any Laboratory Information Management System to the Vial and Analytical Results. Agilent Technologies white paper, publication 5994-7570EN, 2024.