UV-1900i Plus UV-Vis Spectrophotometer

Importance of Topic

The UV-Vis spectrophotometric analysis remains a cornerstone technique in modern analytical chemistry, providing rapid, non-destructive measurements of molecular absorption across the ultraviolet and visible spectrum. It supports routine quality control, reaction monitoring, biomolecular quantitation and compliance with pharmacopeial standards.

Objectives and Overview

This document presents the UV-1900i Plus, a next-generation UV-Vis spectrophotometer designed to combine user-friendly operation with high-performance measurement. It highlights improvements in interface design, measurement modes, data management and regulatory validation support to meet diverse laboratory needs.

Methodology and Instrumentation

The UV-1900i Plus integrates:

• A capacitive touchscreen with stylus and multi-language menus for intuitive control
• Six measurement modes: photometric, spectral scan, quantitation, kinetics, time course and biomethod
• Low-stray-light diffraction gratings (<0.5% at 198 nm) and 1 nm wavelength resolution
• Ultra-fast scanning (up to 29 000 nm/min) for real-time spectral kinetics
• Integrated network and USB connectivity, barcode and keyboard entry
• Assist, shutdown/wakeup and automatic startup validation functions
• LabSolutions UV-Vis software suite (DB and CS versions) for data acquisition, spectral evaluation, report generation and ER/ES-compliant data management

Main Results and Discussion

Key performance highlights include:

• Linear calibration of acetic acid at 200 nm (R²=0.9998) maintaining accuracy up to 2 Abs due to low stray light
• Temporal tracking of silver-nanoparticle aggregation under ultra-fast scan, demonstrating clear absorbance shifts at 400 nm and 480 nm within seconds
• Photometric repeatability of 0.0002 Abs and a caffeine detection limit of 0.0051 mg/L at 273 nm
• Validation tests for wavelength accuracy, drift, noise, flatness and pharmacopeial compliance (JP/USP/EP) executed via touchscreen or PC software

Benefits and Practical Applications

The UV-1900i Plus delivers:

• Streamlined daily operation with assist prompts, minimal menu navigation and automated power management
• Enhanced productivity through simultaneous data measurement, real-time Excel export and automatic spectral pass/fail evaluation
• Robust data integrity via database storage, version control and multi-instrument integration (LC, GC, AA, FTIR, ICPMS, etc.)
• Application versatility in food dye screening, DNA quantitation with Nano Stick/TrayCell, color analysis and enzyme kinetics

Future Trends and Potential Uses

Emerging developments are likely to include:

• Integration of IoT and AI-driven self-diagnostics for predictive maintenance and unattended operation
• Expanded cloud-based data services for global collaboration and remote method deployment
• Advanced multi-parameter assays combining UV-Vis with complementary techniques in a unified digital ecosystem

Conclusion

The Shimadzu UV-1900i Plus represents a significant step forward in UV-Vis spectroscopy, addressing the evolving demands of analytical laboratories for speed, accuracy, ease of use and regulatory compliance. By uniting refined hardware, smart software and networked data management, it supports a broad range of research and QA/QC workflows.

Reference

Shimadzu Corporation. UV-1900i Plus UV-Vis Spectrophotometer Brochure, C101-E181, 2025.