Clarity Control Module GILSON 203B/204

Importance of Topic

Automated fraction collection in liquid chromatography workflows is essential for isolating and analyzing discrete sample portions with high precision. Integrating a fraction collector directly into chromatography software improves reproducibility, reduces manual intervention and supports demanding QA/QC and research applications.

Objectives and Overview

This document outlines the configuration and use of the Gilson 203B/204 fraction collector under the DataApex Clarity chromatography station. It covers hardware connections, software setup, method programming and operational monitoring to achieve reliable, software-driven fraction collection.

Instrumentation

• Gilson 203B/204 fraction collector
• DataApex Clarity chromatography station with LC Control license
• GSIOC-to-RS232 converter or equivalent cable adapter
• Available serial COM port or MultiCOM adapter

Methodology

The procedure begins by connecting the fraction collector via GSIOC protocol converted to RS232. In Clarity System Configuration, the UNI Ruby control module is added and the GILSON203BFC.RB script is selected. Communication parameters such as port, baud rate and GSIOC ID are set via the DataApex UNI Setup dialog. Chromatographic methods include two dedicated tabs: the Fraction Table for timed or signal-triggered collection and the Vial Numbers tab for vial sequencing, reset rules and end-of-run handling.

Main Results and Discussion

Key software features include:

• Flow rate and delay volume settings to prevent overflow and synchronize valve switching
• Flexible signal conditions (level, slope or combined) for precise triggering of start and stop events
• Data filtering controls to accommodate detector noise and sampling rates
• Vial management options including automatic waste during vial change, peak-top vial switching and continuous collection mode
• Device Monitor window for real-time control and manual overrides
• Graphical display of fraction events and expected delays in the Data Acquisition window
• Customizable reporting of all method parameters and fraction records
• Troubleshooting support via configurable communication logging in COMMDRV.INI

Benefits and Practical Applications

By fully integrating the Gilson 203B/204 with Clarity software, laboratories gain automated, reproducible fraction collection linked directly to chromatographic data. This facilitates downstream sample analysis, reduces errors and streamlines high-throughput workflows in pharmaceuticals, environmental monitoring and biochemical research.

Future Trends and Opportunities

Future advancements may include enhanced signal processing algorithms using machine learning for more robust peak detection, tighter integration with laboratory information management systems (LIMS) and remote or cloud-based monitoring. Development of universal drivers could further simplify multi-vendor instrument networks.

Conclusion

The outlined configuration enables reliable software control of the Gilson 203B/204 fraction collector, offering precise timing, comprehensive method programming and full integration with DataApex Clarity. This solution meets modern demands for automation and traceability in analytical laboratories.