Clarity Control Module R-BIOPHARM RIDACREST

Significance of the Topic

Automated sample preparation and synchronization of auxiliary devices are critical to achieving reproducible, high-throughput chromatographic analyses. The R-Biopharm RIDACREST control module allows direct integration of sample preparation equipment into the Clarity chromatography station, enabling fully managed workflows from sample exchange to injection.

Objectives and Overview of the Manual

This document explains how to install, configure and operate the RIDACREST control module for Clarity. It covers hardware requirements, serial and digital I/O wiring, Clarity system setup, method parameterization, real-time monitoring and troubleshooting procedures.

Instrumentation

• Spark HPD (High Pressure Dispenser)
• Spark ACE (Automatic Cartridge Exchanger)
• Spark Alias autosampler
• Clarity chromatography station with AS control module (p/n A26)
• Serial DB9F-DB9M cable (p/n SK02) and optional Digital Input Device (p/n DID01)

Methodology and Instrumentation Configuration

The module communicates with the RIDACREST system via RS-232, LAN or USB. Each device (HPD, ACE, Alias) is assigned a unique ID set on its back panel. Digital inputs/outputs on each instrument permit external start triggers or feedback to other lab instruments. In Clarity’s System Configuration dialog, the RIDACREST auxiliary device is added, communication parameters are auto-detected and mapped to Clarity’s external start and ready signals. In Method Setup, a new “Aux. Devices” tab generates time programs for ACE and HPD, defining actions (cartridge exchange, valve switching, aspiration/dispense cycles) and synchronization steps (Wait for, Delay Time, Inject Marker) within user methods.

Main Features and Discussion

The control module enables:

• Synchronized multi-step sample preparation across ACE, HPD and Alias
• Graphical or sequence-based cartridge selection with offset adjustment
• Programmable auxiliary outputs and digital I/O for custom triggers
• User Program table in the Alias method for full automation of vial handling, washing, valve control and injection signaling
• Real-time monitoring via Device Monitor panes showing device status, pressure, syringe volumes and manual control buttons for maintenance tasks
• Automated report generation with embedded instrument method and configuration details

Benefits and Practical Applications

The RIDACREST module streamlines complex sample workflows, reduces manual intervention and improves method traceability. Laboratories performing immunoaffinity cartridge clean-up, high-pressure dispensing or large-batch autosampling benefit from:

• Enhanced throughput by chaining sample preparation steps directly into the chromatographic sequence
• Improved reproducibility through tight synchronization and digital feedback
• Comprehensive audit trails and GLP-compliant reporting of each instrument action
• Flexible integration with existing detectors or digital input cards for external synchronization

Future Trends and Applications

Future developments may include additional RIDACREST modules (e.g., online filtration, automated derivatization) and enhanced software features such as digital I/O naming, advanced error recovery routines and cloud-based remote monitoring. Integration with emerging mass spectrometry and multidimensional chromatography platforms will further expand application scope.

Conclusion

The R-Biopharm RIDACREST control module provides a robust, unified interface for complex sample preparation devices within the Clarity environment. Its flexible time programming, real-time monitoring and comprehensive reporting enable seamless, automated workflows, boosting laboratory efficiency and data integrity.