Clarity Control Module SPARK MIDAS

Importance of the Topic

Automated sample preparation and injection are fundamental in modern chromatographic analysis, ensuring high reproducibility, reducing human error and enhancing laboratory throughput. Integration of an autosampler into the control software enables precise timing, consistent handling and full documentation of sample preparation parameters within chromatographic data files.

Objectives and Study Overview

This application note details the setup and operation of the Spark Holland Midas autosampler under the DataApex Clarity chromatography station. It covers hardware requirements, serial communication wiring, software configuration, method creation, real-time control during sequences and troubleshooting procedures. The goal is to provide a comprehensive, step-by-step guide for seamless integration of the autosampler into routine analyses.

Applied Methodology and Instrumentation

The manual prescribes a straightforward hardware installation:

• Ensure Clarity with AS Control Module (p/n A26) and firmware ≥2.05.
• Use a free PC serial COM port or a MultiCOM adapter.
• Select appropriate cable: straight DB9F-DB9M for Multi Link port versions or cross DB9F-DB25M for older units.
• Wire external start marker for LC synchronization.

Software configuration within Clarity involves:

• Adding the Midas control module under System Configuration, matching COM port and device ID (60–69).
• Assigning digital input/output lines for external start and ready signals.
• Enabling or disabling Sequence Mode to send complete injection sequences at once or individual injections on demand.

Instrument method development is organized in four tabs:

1. Mode, Time and Temp. – Defines injection mode (none, partial loopfill, flush loop, µl pick-up), analysis time, loop volume, wash routines and optional oven/tray cooling settings.
2. Timed Events – Configures auxiliary outputs and temperature setpoints as a function of runtime with up to four on/off events per channel.
3. Mix Table – Automates pre-injection derivatization or dilution by aspirating reagents, mixing cycles and timed waits, directing transfers to sample or destination vials.
4. System Settings – Specifies syringe and tubing volumes, tray and vial types, syringe speed, air segments, headspace pressure, alarm buzzer and missing-vial behavior.

Main Results and Discussion

Key features demonstrated include direct, real-time control of all autosampler functions from Clarity, with full parameter logging into chromatogram metadata. The Sequence Mode streamlines multi-injection series, while single-injection mode retains flexibility for unscheduled runs. The Mix Table and Timed Events extend the autosampler’s role beyond injection to complex sample preprocessing tasks.

Benefits and Practical Applications

The integrated solution delivers:

• Enhanced reproducibility through software-driven control of volumes, timings and temperatures.
• Reduced manual intervention and error in sample prep, derivatization and reagent handling.
• Comprehensive audit trails by embedding autosampler settings within chromatographic results.
• Flexible workflows—from routine injections to advanced dilutions or derivatizations—in a single platform.

Future Trends and Applications

Advances may include remote monitoring and control via networked interfaces, Internet of Things connectivity for real-time diagnostics, expanded reagent and vial handling modules and AI-driven method optimization. Integration with laboratory information management systems will further streamline data flow and compliance in high-throughput environments.

Conclusion

The Spark Holland Midas control module for Clarity provides a robust framework for automating complex sample preparation and injection protocols. By combining detailed method configuration, full sequence control and integrated reporting, the system enhances analytical performance and laboratory productivity.

Instrumentation Used

• DataApex Clarity Chromatography Station with AS Control Module (p/n A26)
• Spark Holland Midas Autosampler (firmware ≥2.05)
• Straight serial DB9F-DB9M cable (p/n SK02) or cross DB9F-DB25M cable (p/n SK03)
• Optional MultiCOM adapter for multiple COM ports (p/n MC01)
• PC with available serial COM port