Clarity Control Module TELEDYNE FOXY ISCO 200

Significance of the Topic

The integration of automated fraction collectors into modern chromatography workflows is essential for high-throughput purification, reliable sample handling and consistent data logging. Direct control of fraction collectors from chromatography software enhances precision, reduces manual intervention and supports rigorous QA/QC standards.

Study Objectives and Overview

This document presents the implementation and configuration of the Teledyne Foxy ISCO 200 fraction collector within the DataApex Clarity chromatography environment. It covers hardware requirements, software setup, method development and real-time monitoring, aiming to provide a seamless interface for fractionation tasks.

Methodology and Instrumentation

• Instrumental Setup
  • Teledyne Foxy ISCO 200 fraction collector configured with firmware 2135-061 or 2135-063
  • RS-232 serial connection via DB9F–DB25M straight cable and COM port or MultiCOM adapter
  • DataApex Clarity software with LC control license and UNI Ruby module script (TELEDYNEISCOFOXY200FC.RB)
• Software Configuration
  • Define serial port parameters (baud rate 9600, device ID 5)
  • Add control module in System Configuration, assign to LC instrument type
  • Select communication script, set custom device name and rack layouts in UNI Setup
• Method Development
  • Fraction Table defines flow rate, delay volumes, collection triggers (level, slope or combined) and filter settings
  • Vial Numbers tab configures start/stop vial indices, reset conditions and overflow handling (waste or shutdown)

Key Results and Discussion

The module enables precise timing of fraction start and stop events logged into chromatograms and Data Acquisition windows. Real-time Device Monitor displays current state, vial position and allows manual override (Next Vial, Start/Stop Collecting, Go To Position). The report generator captures all method parameters for archiving and audit trails.

Benefits and Practical Applications

• Automated fractionation synchronized with detector signals for preparative separations
• Reduced risk of vial overflow through programmable delay volumes and overflow safeguards
• Comprehensive logging of collection events for traceability
• Adaptable workflows: time-based, signal-triggered or continuous collection modes

Future Trends and Applications

Advances may include Ethernet or USB connectivity, enhanced graphical programming of collection logic, AI-driven peak detection, cloud-based monitoring, integration with laboratory information management systems and multi-channel collector arrays for parallel workflows.

Conclusion

The Teledyne Foxy ISCO 200 control module within Clarity streamlines preparative fractionation by unifying hardware control, method setup and data management. Its flexible trigger options and robust monitoring tools support diverse laboratory requirements.

Reference

• DataApex Ltd. Teledyne Foxy ISCO 200 Clarity Control Module Manual, Rev. M165/90B, November 2024.