Automated MHC-Associated Peptide Enrichment for Immunopeptidomics Analysis Using Agilent AssayMAP Bravo Large Capacity CartridgesApplications | 2020 | Agilent Technologies InstrumentationSample Preparation, LC/TOF, LC/HRMS, LC/MS, LC/MS/MSIndustriesPharma & Biopharma, Proteomics ManufacturerAgilent TechnologiesKey wordsmhc, peptide, peptides, cartridges, byologic, assaymap, crosslinked, antibody, paw, bravo, byonic, byos, motif, were, crosslinking, immunopeptidomics, nanodapter, hla, buffer, using, resin, protein, identified, purification, preference, automated, lysate, cleanup, anti, bed, binding, ctls, histocompatibility, immunoaffinity, complexes, ids, abundance, unique, loading, workflow, cartridge, distribution, affinity, identification, immobilization, counts, showed, associated, enrichment, three
Host Cell Protein Analysis Using Agilent AssayMAP Bravo and 6545XT AdvanceBio LC/Q-TOF
Application NoteBiotherapeutics andBiosimilarsHost Cell Protein Analysis UsingAgilent AssayMAP Bravo and 6545XTAdvanceBio LC/Q-TOFAuthorsLinfeng Wu, Shuai Wu andTe-Wei ChuAgilent Technologies, Inc.,Santa Clara, CA, USAIntroductionUse of biopharmaceuticals such as monoclonal antibodies (mAbs) has beengrowing rapidly. Since biopharmaceuticals are generated from biological sources,some of...
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Automation of Sample Preparation for Accurate and Scalable Quantification and Characterization of Biotherapeutic Proteins Using the Agilent AssayMAP Bravo Platform
Automation of Sample Preparationfor Accurate and ScalableQuantification and Characterizationof Biotherapeutic Proteins Using theAgilent AssayMAP Bravo PlatformApplication NoteAutomated Protein Sample Preparation for LC/MSAuthorsAbstractMichael Bovee, Jason Russell,The Agilent AssayMAP Bravo platform automates a wide variety of LC/MSSteve Murphysample prep workflows on a...
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Rapid Antibody Digestion Enabled by Automated Reversed-Phase Desalting on the Agilent AssayMAP Bravo Platform
Rapid Antibody Digestion Enabled byAutomated Reversed-Phase Desaltingon the Agilent AssayMAP BravoPlatformApplication NoteAuthorsIntroductionMichael Bovee, Adam Krahenbuhl, andMonoclonal antibodies are a rapidly growing class of biotherapeutics whose rigorouscharacterization by mass spectrometry requires reproducible denaturation and digestion.One of the major bottlenecks in this...
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Development of an automated MHC-associated peptide enrichment method for immunopeptidomics analysis using AssayMAP large capacity cartridges
Poster ReprintASMS 2020ThP 412Development of an automatedMHC-associated peptideenrichment method forimmunopeptidomics analysisusing AssayMAP large capacitycartridgesSamuel Pollock1, Shuai Wu2, Jerry Han2, SteveMurphy21Genentech, South San Francisco, CA, USAAgilent Technologies Inc., Santa Clara, CA,USA2IntroductionImmunopeptidomics is generally considered morechallenging than conventional proteomics workflowsfor a number...
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