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Application NoteBiotherapeutics andBiosimilarsHost Cell Protein Analysis UsingAgilent AssayMAP Bravo and 6545XTAdvanceBio LC/Q-TOFAuthorsLinfeng Wu, Shuai Wu andTe-Wei ChuAgilent Technologies, Inc.,Santa Clara, CA, USAIntroductionUse of biopharmaceuticals such as monoclonal antibodies (mAbs) has beengrowing rapidly. Since biopharmaceuticals are generated from biological sources,some of...
Key words
hprp, hprpiterative, iterativeprotein, proteinfractionation, fractionationassaymap, assaymapbravo, bravocounts, countspeptide, peptideunfractionated, unfractionatedxic, xicdynamic, dynamicwere, werehcps, hcpsusing, usingbyologic
Automation of Sample Preparationfor Accurate and ScalableQuantification and Characterizationof Biotherapeutic Proteins Using theAgilent AssayMAP Bravo PlatformApplication NoteAutomated Protein Sample Preparation for LC/MSAuthorsAbstractMichael Bovee, Jason Russell,The Agilent AssayMAP Bravo platform automates a wide variety of LC/MSSteve Murphysample prep workflows on a...
Key words
assaymap, assaymapbravo, bravocartridges, cartridgesaffinity, affinityantibody, antibodypurification, purificationcoverage, coveragechymotrypsin, chymotrypsinpeptide, peptideprotein, proteinplatform, platformeluted, elutedworkflow, workflowworkflows, workflowspeptides
Rapid Antibody Digestion Enabled byAutomated Reversed-Phase Desaltingon the Agilent AssayMAP BravoPlatformApplication NoteAuthorsIntroductionMichael Bovee, Adam Krahenbuhl, andMonoclonal antibodies are a rapidly growing class of biotherapeutics whose rigorouscharacterization by mass spectrometry requires reproducible denaturation and digestion.One of the major bottlenecks in this...
Key words
assaymap, assaymapcartridges, cartridgesdigestion, digestionload, loadantibodies, antibodiesbravo, bravoeluent, eluentwere, wereprotein, proteinrecovery, recoverywqqg, wqqgantibody, antibodycleanup, cleanupdesalted, desaltedeluate
Poster ReprintASMS 2020ThP 412Development of an automatedMHC-associated peptideenrichment method forimmunopeptidomics analysisusing AssayMAP large capacitycartridgesSamuel Pollock1, Shuai Wu2, Jerry Han2, SteveMurphy21Genentech, South San Francisco, CA, USAAgilent Technologies Inc., Santa Clara, CA,USA2 IntroductionImmunopeptidomics is generally considered morechallenging than conventional proteomics workflowsfor a number...
Key words
peptide, peptidemhc, mhcpaw, pawpeptides, peptidesimmunopeptidomics, immunopeptidomicslength, lengthbyonic, byoniclysate, lysateidentified, identifiednanoflow, nanoflowimmunoaffinity, immunoaffinitydistribution, distributionabundance, abundancegranta, grantahla
 

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Application NoteBiotherapeutics andBiosimilarsHost Cell Protein Analysis UsingAgilent AssayMAP Bravo and 6545XTAdvanceBio LC/Q-TOFAuthorsLinfeng Wu, Shuai Wu andTe-Wei ChuAgilent Technologies, Inc.,Santa Clara, CA, USAIntroductionUse of biopharmaceuticals such as monoclonal antibodies (mAbs) has beengrowing rapidly. Since biopharmaceuticals are generated from biological sources,some of...
Key words
hprp, hprpiterative, iterativeprotein, proteinfractionation, fractionationassaymap, assaymapbravo, bravocounts, countspeptide, peptideunfractionated, unfractionatedxic, xicdynamic, dynamicwere, werehcps, hcpsusing, usingbyologic
Automation of Sample Preparationfor Accurate and ScalableQuantification and Characterizationof Biotherapeutic Proteins Using theAgilent AssayMAP Bravo PlatformApplication NoteAutomated Protein Sample Preparation for LC/MSAuthorsAbstractMichael Bovee, Jason Russell,The Agilent AssayMAP Bravo platform automates a wide variety of LC/MSSteve Murphysample prep workflows on a...
Key words
assaymap, assaymapbravo, bravocartridges, cartridgesaffinity, affinityantibody, antibodypurification, purificationcoverage, coveragechymotrypsin, chymotrypsinpeptide, peptideprotein, proteinplatform, platformeluted, elutedworkflow, workflowworkflows, workflowspeptides
Rapid Antibody Digestion Enabled byAutomated Reversed-Phase Desaltingon the Agilent AssayMAP BravoPlatformApplication NoteAuthorsIntroductionMichael Bovee, Adam Krahenbuhl, andMonoclonal antibodies are a rapidly growing class of biotherapeutics whose rigorouscharacterization by mass spectrometry requires reproducible denaturation and digestion.One of the major bottlenecks in this...
Key words
assaymap, assaymapcartridges, cartridgesdigestion, digestionload, loadantibodies, antibodiesbravo, bravoeluent, eluentwere, wereprotein, proteinrecovery, recoverywqqg, wqqgantibody, antibodycleanup, cleanupdesalted, desaltedeluate
Poster ReprintASMS 2020ThP 412Development of an automatedMHC-associated peptideenrichment method forimmunopeptidomics analysisusing AssayMAP large capacitycartridgesSamuel Pollock1, Shuai Wu2, Jerry Han2, SteveMurphy21Genentech, South San Francisco, CA, USAAgilent Technologies Inc., Santa Clara, CA,USA2 IntroductionImmunopeptidomics is generally considered morechallenging than conventional proteomics workflowsfor a number...
Key words
peptide, peptidemhc, mhcpaw, pawpeptides, peptidesimmunopeptidomics, immunopeptidomicslength, lengthbyonic, byoniclysate, lysateidentified, identifiednanoflow, nanoflowimmunoaffinity, immunoaffinitydistribution, distributionabundance, abundancegranta, grantahla
 

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