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MALDI Mass Spectrometry Imaging Reveals Distinct Spatio-Molecular Phospholipid Distributions in Mouse Lungs

 

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Intensity (arb. unit) 132% 0% 100% PS 38:1 m/z 816.5747 ± 0.0122 1/K0 1.433 ± 0.01 Intensity (arb. unit) Mobility [1/K0] 136% 0% 100% PE 20:1_22:6 m/z 816.5517 ± 0.0122 1/K0 1.404 ± 0.01 m/z 1 mm CCS-aware SpatialOMx® enables…
Key words
ccs, ccsmaldi, maldispatialomx, spatialomximaging, imagingaware, awareannotation, annotationtims, timsannotated, annotatedmobility, mobilityannotations, annotationsbucket, bucketpasef, paseflipid, lipidsegmentation, segmentationimages
25 YEARS MALDI High-performance MALDI imaging analysis of on-tissue digested proteins in mammalian FFPE tissues using the Bruker rapifleX MALDI-TOF/TOF In this application note, we describe a workflow for MALDI Mass Spectrometry Imaging (MSI) of proteins from a variety of…
Key words
tissue, tissueffpe, ffpemaldi, maldiimage, imageimaging, imagingsegmentation, segmentationpeptides, peptidestof, tofrapiflex, rapiflexparaffin, paraffinsections, sectionsbruker, brukerscils, scilsstained, stainedwere
Lipid Visualization and Identification Through Collisional Cross Section Aided Correlation of MS Imaging and Ex Situ MS Data for MS-MS Identification Mark Towers and Emmanuelle Claude Waters Corporation, Wilmslow, UK G OA L CCS areas used to compare multiple datasets…
Key words
imaging, imaginglipid, lipidlipids, lipidsccs, ccslipi, lipican, cancollisional, collisionaletha, ethasection, sectionisobaric, isobaricareas, areastissue, tissuedata, datacross, crosssitu
High-speed MALDI-TOF/TOF imaging of mouse brain tissue performed on intact proteins and after on-tissue digestion The unique flexibility of MALDI-TOF/TOF systems facilitates tissue imaging of intact proteins as well as of tryptic peptides resulting from on-tissue digestion. While intact protein…
Key words
imaging, imagingmaldi, malditissue, tissuetof, tofcerebellum, cerebellumintact, intactcortex, cortexhistones, histonesspatial, spatialproteins, proteinspeptides, peptidesprotein, proteinrapiflex, rapiflexbrain, brainmouse
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