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Online 2D-nanoLC hyphenated with high-resolution accurate-mass Orbitrap mass spectrometry for comprehensive and robust proteome profiling

Applications | 2019 | Thermo Fisher ScientificInstrumentation
LC/HRMS, LC/MS, LC/MS/MS, LC/Orbitrap, 2D-LC
Industries
Proteomics
Manufacturer
Thermo Fisher Scientific
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Low-flow On-line 2D LCMS Hyphenated with HRAM MS for Comprehensive Proteome Profiling Oleksandr Boychenko, Christopher Pynn, Wim Decrop, Peter Jehle, Mauro De Pra, Thermo Fisher Scientific, Dornierstr. 4, Germering, Germany, 82205 ABSTRACT Purpose: Develop an automated on-line 2D setup and…
Key words
proteome, proteomeplasma, plasmafractions, fractionsprotein, proteindimension, dimensionloading, loadingpeptides, peptidesapproach, approachlow, lowacidify, acidifyhpg, hpgcrude, crudedigest, digestlcms, lcmscoverage
Comprehensive and Robust Proteome Profiling using Online-2D NanoLC coupled to the Orbitrap Exploris 480 MS Tabiwang N. Arrey1, Runsheng Zheng2, Alexander Boychenko2, Alexander Harder1 1Thermo Fisher Scientific, Bremen, Germany, 2 Thermo Fisher Scientific, Germering, Germany INTRODUCTION RESULTS Although high proteome…
Key words
orthogonality, orthogonalitypeptide, peptideproteome, proteomeseralab, seralabdimension, dimensioncoverages, coveragescarbamidomethylation, carbamidomethylationshotgun, shotgunmanipulation, manipulationdeamidation, deamidationgradually, graduallydeeper, deeperrplc, rplcscx, scxfeasibility
Precise Quantitative Serum LC-MS/MS Profiling: The Impact of Sample Preparation and Sample Source on Biomarker Discovery Studies Alexander Boychenko1, Natalia Govorukhina2, Runsheng Zheng1 1Thermo Fisher Scientific, Germering, Germany of Groningen, Groningen, The Netherlands 2University 1 For Research Use Only. Not…
Key words
seralab, seralabumcg, umcgvariability, variabilityblood, bloodbiomarkers, biomarkersprotein, proteinproteins, proteinsppbp, ppbpfgg, fgghbd, hbdppia, ppiaatlas, atlasgroups, groupslpa, lpasweden
TECHNICAL NOTE 72899 Tandem nanoLC-MS: maximum MS utilization for deep-dive proteomic analysis Table of Contents Authors Christopher Pynn , Alexander Boychenko1, Wim Decrop1, Mike Baynham2, Peter Jehle1, Martin Ruehl1 1 Thermo Fisher Scientific, Germering, Germany 1 Thermo Fisher Scientific, Runcorn,…
Key words
loading, loadingpump, pumpnanolc, nanolctandem, tandemloop, loopnano, nanowash, washcomment, commentvici, vicipeptide, peptidecolumn, columnsample, samplevalve, valveproteomics, proteomicsdive
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