Size-Exclusion Chromatography Analysis of Adeno-Associated Virus (AAV) Preparations Using a 450 Å Diol-Bonded BEH Column and Fluorescence Detection

Applications | 2020 | WatersInstrumentation
Consumables, HPLC, LC columns, GPC/SEC
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Clinical Research
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Waters
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Anion-Exchange Chromatography for Determining Emptyand Full Capsid Contents in Adeno-Associated VirusHua Yang, Stephan Koza, and Weibin ChenWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■Empty and full AAV capsid separationRecombinant adeno-associated virus (AAV) vector has been increasinglyand quantification by Protein-Pakused as a vehicle...
Key words
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Developing the analytics and analytical workflows supporting the analysisof the next generation of biotherapeutic and gene therapies.Scott J. Berger, Ph.D. and Ximo Zhang, Ph.D.Waters Lunch SeminarTuesday January 28, 2020WCBP 2020DiamondProgram Partner©2020 Waters Corporation. COMPANY CONFIDENTIAL1 Ralph Waldo Emersonb. 1803 - d.1882©2020...
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[ APPLICATION NOTEBOOK ]SIZE-EXCLUSIONCHROMATOGRAPHY (SEC)OF PEPTIDES, PROTEINS, AND AAVs [ SIZE-EXCLUSION CHROMATOGRAPHY ] [ SIZE-EXCLUSION CHROMATOGRAPHY ]IntroductionGel-permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel-filtrationchromatography (GFC) are commonly used terms to describe a liquid chromatographic techniquethat separates compounds based on their relative different...
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Application NoteImproved SizeExclusionChromatography ofAdeno-AssociatedViruses (AAVs) WithXBridge™ Premier GTxBEH™ SEC 450 Å 2.5µm ColumnsMateusz Imiolek, Szabolcs Fekete, Matthew A. LauberWaters CorporationAbstractThis application note discusses the use of XBridge™ PremierGTx BEH™ SEC 450 Å 2.5 µm Columns for improved sizeexclusion chromatography (SEC)...
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