Size-Exclusion Chromatography Analysis of Adeno-Associated Virus (AAV) Preparations Using a 450 Å Diol-Bonded BEH Column and Fluorescence DetectionApplications | 2020 | WatersInstrumentation Consumables, HPLC, LC columns, GPC/SECIndustriesClinical ResearchManufacturerWatersKey wordsaav, adeno, virus, flr, hmws, acquity, associated, exclusion, preparations, sec, thyroglo, lmws, chromatography, size, uplc, optimization, fluorescence, protein, bio, xbridge, note, molar, intrinsic, application, represented, low, scheme, detector, unless, minutes, otherwise, noted, bulin, myoglob, overlooking, selfassociated, tryptop, valency, waters, class, forms, persisting, subvisible, multimeric, fragments, dimeric, employed, manufacturing, serotype, serotypes
Anion-Exchange Chromatography for Determining Empty and Full Capsid Contents in Adeno-Associated Virus
Anion-Exchange Chromatography for Determining Emptyand Full Capsid Contents in Adeno-Associated VirusHua Yang, Stephan Koza, and Weibin ChenWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■Empty and full AAV capsid separationRecombinant adeno-associated virus (AAV) vector has been increasinglyand quantification by Protein-Pakused as a vehicle...
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Optimizing Adeno-Associated Virus (AAV) Capsid Protein Analysis Using UPLC and UPLC-MS
Optimizing Adeno-Associated Virus (AAV) Capsid Protein AnalysisUsing UPLC and UPLC-MSXimo Zhang, Stephan Koza, Ying Qing Yu, and Weibin ChenWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■INTRODUCTIONAn optimized LC-MS solution for theRecombinant adeno-associated viruses (rAAVs) are the most widelyseparation and intact mass analysisused...
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Developing the analytics and analytical workflows supporting the analysis of the next generation of biotherapeutic and gene therapies
Developing the analytics and analytical workflows supporting the analysisof the next generation of biotherapeutic and gene therapies.Scott J. Berger, Ph.D. and Ximo Zhang, Ph.D.Waters Lunch SeminarTuesday January 28, 2020WCBP 2020DiamondProgram Partner©2020 Waters Corporation. COMPANY CONFIDENTIAL1Ralph Waldo Emersonb. 1803 - d.1882©2020...
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Rapid AAV Concentration Determination Using Size-Exclusion Chromatography with Fluorescence and UV Dual Detection
Application NoteRapid AAV Concentration DeterminationUsing Size-Exclusion Chromatography withFluorescence and UV Dual DetectionStephan M. Koza, Weibin ChenWaters CorporationAbstractA method using an SEC guard column as an online buffer exchange device prior to intrinsic proteinfluorescence detection was demonstrated to be effective for...
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