Size-Exclusion Chromatography Analysis of Adeno-Associated Virus (AAV) Preparations Using a 450 Å Diol-Bonded BEH Column and Fluorescence Detection | LabRulez LCMS
 

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Anion-Exchange Chromatography for Determining Emptyand Full Capsid Contents in Adeno-Associated VirusHua Yang, Stephan Koza, and Weibin ChenWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■Empty and full AAV capsid separationRecombinant adeno-associated virus (AAV) vector has been increasinglyand quantification by Protein-Pakused as a vehicle...
Key words
empty, emptycapsid, capsidcapsids, capsidsaav, aavaex, aexfull, fullvirus, virusadeno, adenoserotypes, serotypesanion, anionassociated, associateddetermining, determiningminutes, minutesexchange, exchangeres
Optimizing Adeno-Associated Virus (AAV) Capsid Protein AnalysisUsing UPLC and UPLC-MSXimo Zhang, Stephan Koza, Ying Qing Yu, and Weibin ChenWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■INTRODUCTIONAn optimized LC-MS solution for theRecombinant adeno-associated viruses (rAAVs) are the most widelyseparation and intact mass analysisused...
Key words
capsid, capsidaav, aavadeno, adenouplc, uplcvirus, virusassociated, associatedproteins, proteinsoptimizing, optimizingraav, raavtherapy, therapymass, massflr, flrgene, geneacquity, acquityviral
Developing the analytics and analytical workflows supporting the analysisof the next generation of biotherapeutic and gene therapies.Scott J. Berger, Ph.D. and Ximo Zhang, Ph.D.Waters Lunch SeminarTuesday January 28, 2020WCBP 2020DiamondProgram Partner©2020 Waters Corporation. COMPANY CONFIDENTIAL1 Ralph Waldo Emersonb. 1803 - d.1882©2020...
Key words
capsid, capsidaav, aavempty, emptydevelopment, developmentgene, geneprotein, proteincapsids, capsidstherapy, therapyproteins, proteinsmeasure, measureiex, iexfull, fullrplc, rplcsec, secmonitoring
[ APPLICATION NOTEBOOK ]SIZE-EXCLUSIONCHROMATOGRAPHY (SEC)OF PEPTIDES, PROTEINS, AND AAVs [ SIZE-EXCLUSION CHROMATOGRAPHY ] [ SIZE-EXCLUSION CHROMATOGRAPHY ]IntroductionGel-permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel-filtrationchromatography (GFC) are commonly used terms to describe a liquid chromatographic techniquethat separates compounds based on their relative different...
Key words
sec, secexclusion, exclusionsize, sizeuplc, uplcacquity, acquitychromatography, chromatographyprotein, proteinaggregates, aggregateshplc, hplcdispersion, dispersionmonomer, monomerhmws, hmwscolumn, columnigg, iggantibody
 

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Anion-Exchange Chromatography for Determining Emptyand Full Capsid Contents in Adeno-Associated VirusHua Yang, Stephan Koza, and Weibin ChenWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■Empty and full AAV capsid separationRecombinant adeno-associated virus (AAV) vector has been increasinglyand quantification by Protein-Pakused as a vehicle...
Key words
empty, emptycapsid, capsidcapsids, capsidsaav, aavaex, aexfull, fullvirus, virusadeno, adenoserotypes, serotypesanion, anionassociated, associateddetermining, determiningminutes, minutesexchange, exchangeres
Optimizing Adeno-Associated Virus (AAV) Capsid Protein AnalysisUsing UPLC and UPLC-MSXimo Zhang, Stephan Koza, Ying Qing Yu, and Weibin ChenWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■INTRODUCTIONAn optimized LC-MS solution for theRecombinant adeno-associated viruses (rAAVs) are the most widelyseparation and intact mass analysisused...
Key words
capsid, capsidaav, aavadeno, adenouplc, uplcvirus, virusassociated, associatedproteins, proteinsoptimizing, optimizingraav, raavtherapy, therapymass, massflr, flrgene, geneacquity, acquityviral
Developing the analytics and analytical workflows supporting the analysisof the next generation of biotherapeutic and gene therapies.Scott J. Berger, Ph.D. and Ximo Zhang, Ph.D.Waters Lunch SeminarTuesday January 28, 2020WCBP 2020DiamondProgram Partner©2020 Waters Corporation. COMPANY CONFIDENTIAL1 Ralph Waldo Emersonb. 1803 - d.1882©2020...
Key words
capsid, capsidaav, aavempty, emptydevelopment, developmentgene, geneprotein, proteincapsids, capsidstherapy, therapyproteins, proteinsmeasure, measureiex, iexfull, fullrplc, rplcsec, secmonitoring
[ APPLICATION NOTEBOOK ]SIZE-EXCLUSIONCHROMATOGRAPHY (SEC)OF PEPTIDES, PROTEINS, AND AAVs [ SIZE-EXCLUSION CHROMATOGRAPHY ] [ SIZE-EXCLUSION CHROMATOGRAPHY ]IntroductionGel-permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel-filtrationchromatography (GFC) are commonly used terms to describe a liquid chromatographic techniquethat separates compounds based on their relative different...
Key words
sec, secexclusion, exclusionsize, sizeuplc, uplcacquity, acquitychromatography, chromatographyprotein, proteinaggregates, aggregateshplc, hplcdispersion, dispersionmonomer, monomerhmws, hmwscolumn, columnigg, iggantibody
 

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