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Anion-Exchange Chromatography for Determining Emptyand Full Capsid Contents in Adeno-Associated VirusHua Yang, Stephan Koza, and Weibin ChenWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■Empty and full AAV capsid separationRecombinant adeno-associated virus (AAV) vector has been increasinglyand quantification by Protein-Pakused as a vehicle...
Key words
empty, emptycapsid, capsidcapsids, capsidsaav, aavaex, aexvirus, virusfull, fulladeno, adenoserotypes, serotypesanion, anionassociated, associatedminutes, minutesdetermining, determiningexchange, exchangeres
Optimizing Adeno-Associated Virus (AAV) Capsid Protein AnalysisUsing UPLC and UPLC-MSXimo Zhang, Stephan Koza, Ying Qing Yu, and Weibin ChenWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■INTRODUCTIONAn optimized LC-MS solution for theRecombinant adeno-associated viruses (rAAVs) are the most widelyseparation and intact mass analysisused...
Key words
capsid, capsidaav, aavadeno, adenouplc, uplcvirus, virusassociated, associatedproteins, proteinsoptimizing, optimizingraav, raavtherapy, therapyflr, flrmass, massgene, geneacquity, acquityprotein
Developing the analytics and analytical workflows supporting the analysisof the next generation of biotherapeutic and gene therapies.Scott J. Berger, Ph.D. and Ximo Zhang, Ph.D.Waters Lunch SeminarTuesday January 28, 2020WCBP 2020DiamondProgram Partner©2020 Waters Corporation. COMPANY CONFIDENTIAL1 Ralph Waldo Emersonb. 1803 - d.1882©2020...
Key words
capsid, capsidaav, aavempty, emptydevelopment, developmentgene, geneprotein, proteincapsids, capsidstherapy, therapyproteins, proteinsiex, iexmeasure, measurerplc, rplcfull, fullmonitoring, monitoringcharacterization
Application NoteRapid AAV Concentration DeterminationUsing Size-Exclusion Chromatography withFluorescence and UV Dual DetectionStephan M. Koza, Weibin ChenWaters Corporation AbstractA method using an SEC guard column as an online buffer exchange device prior to intrinsic proteinfluorescence detection was demonstrated to be effective for...
Key words
aav, aavcapsid, capsidflr, flrχfull, χfullsec, secintrinsic, intrinsicconcentration, concentrationmay, mayempty, emptyfluorescence, fluorescenceuplc, uplcacquity, acquityssdna, ssdnaprotein, proteinversus
 

Similar PDF

Anion-Exchange Chromatography for Determining Emptyand Full Capsid Contents in Adeno-Associated VirusHua Yang, Stephan Koza, and Weibin ChenWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■Empty and full AAV capsid separationRecombinant adeno-associated virus (AAV) vector has been increasinglyand quantification by Protein-Pakused as a vehicle...
Key words
empty, emptycapsid, capsidcapsids, capsidsaav, aavaex, aexvirus, virusfull, fulladeno, adenoserotypes, serotypesanion, anionassociated, associatedminutes, minutesdetermining, determiningexchange, exchangeres
Optimizing Adeno-Associated Virus (AAV) Capsid Protein AnalysisUsing UPLC and UPLC-MSXimo Zhang, Stephan Koza, Ying Qing Yu, and Weibin ChenWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■INTRODUCTIONAn optimized LC-MS solution for theRecombinant adeno-associated viruses (rAAVs) are the most widelyseparation and intact mass analysisused...
Key words
capsid, capsidaav, aavadeno, adenouplc, uplcvirus, virusassociated, associatedproteins, proteinsoptimizing, optimizingraav, raavtherapy, therapyflr, flrmass, massgene, geneacquity, acquityprotein
Developing the analytics and analytical workflows supporting the analysisof the next generation of biotherapeutic and gene therapies.Scott J. Berger, Ph.D. and Ximo Zhang, Ph.D.Waters Lunch SeminarTuesday January 28, 2020WCBP 2020DiamondProgram Partner©2020 Waters Corporation. COMPANY CONFIDENTIAL1 Ralph Waldo Emersonb. 1803 - d.1882©2020...
Key words
capsid, capsidaav, aavempty, emptydevelopment, developmentgene, geneprotein, proteincapsids, capsidstherapy, therapyproteins, proteinsiex, iexmeasure, measurerplc, rplcfull, fullmonitoring, monitoringcharacterization
Application NoteRapid AAV Concentration DeterminationUsing Size-Exclusion Chromatography withFluorescence and UV Dual DetectionStephan M. Koza, Weibin ChenWaters Corporation AbstractA method using an SEC guard column as an online buffer exchange device prior to intrinsic proteinfluorescence detection was demonstrated to be effective for...
Key words
aav, aavcapsid, capsidflr, flrχfull, χfullsec, secintrinsic, intrinsicconcentration, concentrationmay, mayempty, emptyfluorescence, fluorescenceuplc, uplcacquity, acquityssdna, ssdnaprotein, proteinversus
 

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