Size Exclusion Chromatography Method Development of NIST mAb Using an Agilent AdvanceBio SEC 200 Å 1.9 μm ColumnApplications | 2019 | Agilent TechnologiesInstrumentation Consumables, LC columns, GPC/SECIndustriesPharma & BiopharmaManufacturerAgilent TechnologiesKey wordsbuffer, nacl, dimer, hwm, monomer, mobile, phase, compositions, resolution, quaternary, sec, hmw, phosphate, sodium, asymmetry, development, symmetry, advancebio, pump, different, screening, bio, stock, qin, veronica, peak, optimization, were, conditions, shape, inert, evaluated, monobasic, effect, dibasic, demonstrates, software, method, advisor, following, calculated, negligible, size, gradually, biologics, variants, continues, screened, combinations, filtered
Aggregate and Fragment Analysis - Agilent BioHPLC Columns Application Compendium
Agilent-NISTmAbAggregate andFragment AnalysisAgilent BioHPLC ColumnsApplication CompendiumContentsAgilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted fromNISTmAb RM 8671 batch. Quality control (QC) testing is performed usingAgilent LC-MS system. QC batch release test includes aggregate profile, chargevariants and intact mass information. A certificate of...
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Mobile Phase Optimization in SEC Method Development
Application NotePharma & BiopharmaMobile Phase Optimization in SECMethod DevelopmentAuthorRichard HurteauAgilent Technologies, Inc.,Wilmington, DE, USAAbstractAggregation of monoclonal antibody (mAb) products has an adverse effect ontheir efficacy as therapeutic agents. Size exclusion chromatography (SEC) is ahighly effective method for the detection of...
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Agilent BioHPLC Columns - Characterization of NIST Monoclonal Antibody Critical Quality Attributes - Application Compendium
Agilent BioHPLC ColumnsCharacterization of NIST MonoclonalAntibody Critical Quality AttributesApplication CompendiumContentsAgilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted fromNISTmAb RM 8671 batch. Quality control (QC) testing is performed usingAgilent LC-MS system. QC batch release test includes aggregate profile, chargevariants and intact mass...
mab, mabglycan, glycanadvancebio, advancebiomapping, mappingpeptide, peptidecounts, countsmonoclonal, monoclonalagilent, agilentglycans, glycansmin, minprotein, proteinnistmab, nistmabmass, masscolumn, columnantibody
A Comprehensive Workflow to Optimize and Execute Protein Aggregate Studies
A Comprehensive Workflow toOptimize and Execute ProteinAggregate StudiesCombining Size Exclusion Chromatography with MethodDevelopment and Light ScatteringApplication NoteBiotherapeutics and BiosimilarsAuthorsAbstractAndy Coffey and Matthew RainThis Application Note illustrates a comprehensive aggregate analysis workflow to:Agilent Technologies, Inc•Optimize mobile phase conditions for high-performance size...
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