Size Exclusion Chromatography Method Development of NIST mAb Using an Agilent AdvanceBio SEC 200 Å 1.9 μm ColumnApplications | 2019 | Agilent TechnologiesInstrumentation Consumables, LC columns, GPC/SECIndustriesPharma & BiopharmaManufacturerAgilent TechnologiesKey wordsbuffer, nacl, dimer, hwm, monomer, mobile, phase, compositions, resolution, quaternary, sec, hmw, phosphate, sodium, asymmetry, development, symmetry, advancebio, pump, different, screening, bio, stock, qin, veronica, peak, optimization, were, conditions, shape, inert, evaluated, monobasic, effect, dibasic, demonstrates, software, method, advisor, following, calculated, negligible, size, gradually, biologics, variants, continues, screened, combinations, filtered
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Aggregate and Fragment Analysis - Agilent BioHPLC Columns Application Compendium
2020|Agilent Technologies|Guides
Agilent-NISTmAbAggregate andFragment AnalysisAgilent BioHPLC ColumnsApplication Compendium ContentsAgilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted fromNISTmAb RM 8671 batch. Quality control (QC) testing is performed usingAgilent LC-MS system. QC batch release test includes aggregate profile, chargevariants and intact mass information. A certificate of...
Key words
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Application NotePharma & BiopharmaMobile Phase Optimization in SECMethod DevelopmentAuthorRichard HurteauAgilent Technologies, Inc.,Wilmington, DE, USAAbstractAggregation of monoclonal antibody (mAb) products has an adverse effect ontheir efficacy as therapeutic agents. Size exclusion chromatography (SEC) is ahighly effective method for the detection of...
Key words
mau, maumin, minigg, iggphosphate, phosphatekda, kdaaggregation, aggregationinsulin, insulinbuffer, bufferadc, adccytochrome, cytochromeproteins, proteinscolumns, columnstherapeutic, therapeuticnriu, nriupeak
Agilent BioHPLC Columns - Characterization of NIST Monoclonal Antibody Critical Quality Attributes - Application Compendium
2019|Agilent Technologies|Guides
Agilent BioHPLC ColumnsCharacterization of NIST MonoclonalAntibody Critical Quality AttributesApplication Compendium ContentsAgilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted fromNISTmAb RM 8671 batch. Quality control (QC) testing is performed usingAgilent LC-MS system. QC batch release test includes aggregate profile, chargevariants and intact mass...
Key words
mab, mabglycan, glycanadvancebio, advancebiomapping, mappingpeptide, peptidecounts, countsmonoclonal, monoclonalagilent, agilentglycans, glycansmin, minprotein, proteinnistmab, nistmabmass, masscolumn, columnantibody
A Comprehensive Workflow to Optimize and Execute Protein Aggregate Studies
2017|Agilent Technologies|Applications
A Comprehensive Workflow toOptimize and Execute ProteinAggregate StudiesCombining Size Exclusion Chromatography with MethodDevelopment and Light ScatteringApplication NoteBiotherapeutics and BiosimilarsAuthorsAbstractAndy Coffey and Matthew RainThis Application Note illustrates a comprehensive aggregate analysis workflow to:Agilent Technologies, Inc•Optimize mobile phase conditions for high-performance size...
Key words
rituximab, rituximabrituxumab, rituxumabsec, secbiosimilar, biosimilarmobile, mobileaggregate, aggregatebio, bioaggregates, aggregatesbuffer, bufferdetector, detectorresponse, responseinnovator, innovatortime, timemin, minphase
