Analysis of Pharmaceuticals and Personal Care Products in Groundwater by Online SPE and LC/MS/MS
Applications | 2017 | Agilent TechnologiesInstrumentation
Environmental monitoring of trace pharmaceuticals and personal care products in groundwater is critical for assessing water quality and public health risks. Low concentration pollutants require sensitive, efficient analytical approaches to detect ng/L levels.
This study aimed to develop and apply an automated online solid phase extraction (SPE) coupled with liquid chromatography-tandem mass spectrometry (LC/MS/MS) to identify and quantify seven target compounds in groundwater. Over 100 samples from alluvial wells in the South Platte River valley were analyzed to compare sampling techniques and evaluate method performance.
A 900 µL aliquot of filtered groundwater was preconcentrated on polymeric PLRP-S cartridges using the Agilent 1290 Infinity Flexible Cube system, followed by separation on an Agilent Poroshell 120 EC-C8 column and detection with an Agilent 6460 triple quadrupole MS. Sample draw, wash, and elution steps were fully automated with dual cartridges to minimize cycle time.
Recoveries for six analytes ranged from 86 to 164%, with method detection limits of 1–10 ng/L. Gabapentin showed lower recovery (34%) due to polarity issues. Analysis of well 009 detected all seven compounds, with sucralose at 3,360 ng/L and other analytes between 5 and 279 ng/L. Sampling variability (up to 100% concentration change) exceeded analytical variability (~10%), highlighting the impact of field collection.
Automated online SPE reduces solvent exposure, operator error, and analysis time (<20 min/sample) while requiring minimal sample volume (900 µL). The method supports high-throughput monitoring of trace contaminants in environmental water.
Integration of isotopically labeled surrogates for problematic analytes, exploration of alternative sorbents for polar compounds, and expansion to broader contaminant panels will enhance method robustness. Coupling with field-deployable sampling devices could facilitate real-time water quality assessment.
The automated online SPE–LC/MS/MS workflow delivers sensitive, rapid quantitation of pharmaceuticals and personal care products in groundwater using small sample volumes. It offers a cost-effective, reproducible solution for environmental monitoring programs.
No explicit literature cited; methodology and instrument specifications are based on manufacturer and standard practice.
Sample Preparation, LC/MS, LC/MS/MS, LC/QQQ
IndustriesEnvironmental
ManufacturerAgilent Technologies
Summary
Significance of the Topic
Environmental monitoring of trace pharmaceuticals and personal care products in groundwater is critical for assessing water quality and public health risks. Low concentration pollutants require sensitive, efficient analytical approaches to detect ng/L levels.
Study Objectives and Overview
This study aimed to develop and apply an automated online solid phase extraction (SPE) coupled with liquid chromatography-tandem mass spectrometry (LC/MS/MS) to identify and quantify seven target compounds in groundwater. Over 100 samples from alluvial wells in the South Platte River valley were analyzed to compare sampling techniques and evaluate method performance.
Methodology and Instrumentation
A 900 µL aliquot of filtered groundwater was preconcentrated on polymeric PLRP-S cartridges using the Agilent 1290 Infinity Flexible Cube system, followed by separation on an Agilent Poroshell 120 EC-C8 column and detection with an Agilent 6460 triple quadrupole MS. Sample draw, wash, and elution steps were fully automated with dual cartridges to minimize cycle time.
Main Results and Discussion
Recoveries for six analytes ranged from 86 to 164%, with method detection limits of 1–10 ng/L. Gabapentin showed lower recovery (34%) due to polarity issues. Analysis of well 009 detected all seven compounds, with sucralose at 3,360 ng/L and other analytes between 5 and 279 ng/L. Sampling variability (up to 100% concentration change) exceeded analytical variability (~10%), highlighting the impact of field collection.
Benefits and Practical Applications of the Method
Automated online SPE reduces solvent exposure, operator error, and analysis time (<20 min/sample) while requiring minimal sample volume (900 µL). The method supports high-throughput monitoring of trace contaminants in environmental water.
Future Trends and Applications
Integration of isotopically labeled surrogates for problematic analytes, exploration of alternative sorbents for polar compounds, and expansion to broader contaminant panels will enhance method robustness. Coupling with field-deployable sampling devices could facilitate real-time water quality assessment.
Conclusion
The automated online SPE–LC/MS/MS workflow delivers sensitive, rapid quantitation of pharmaceuticals and personal care products in groundwater using small sample volumes. It offers a cost-effective, reproducible solution for environmental monitoring programs.
References
No explicit literature cited; methodology and instrument specifications are based on manufacturer and standard practice.
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