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An Integrated Workflow for Intact and Subunits of Monoclonal Antibody Accurate Mass Measurements

Applications | 2018 | Agilent TechnologiesInstrumentation
Sample Preparation, LC/TOF, LC/HRMS, LC/MS, LC/MS/MS
Industries
Pharma & Biopharma
Manufacturer
Agilent Technologies
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Agilent-NISTmAb Intact & Subunit Analysis Using Reversed Phase Agilent BioHPLC Columns Application Compendium Contents Agilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted from NISTmAb RM 8671 batch. Quality control (QC) testing is performed using Agilent LC-MS system. QC batch release test…
Key words
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Agilent BioHPLC Columns Characterization of NIST Monoclonal Antibody Critical Quality Attributes Application Compendium Contents Agilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted from NISTmAb RM 8671 batch. Quality control (QC) testing is performed using Agilent LC-MS system. QC batch release test…
Key words
mab, mabglycan, glycanadvancebio, advancebiocounts, countsmapping, mappingpeptide, peptideagilent, agilentmonoclonal, monoclonalglycans, glycansmin, mincolumn, columnprotein, proteinmass, massnistmab, nistmabassaymap
Application Note Pharma & Biopharma A Comparative Study of the Intact Mass, Subunit Mass, and Released Glycans of Two Rituximab Biosimilars Using High-Resolution LC/MS Author Brian Liau Agilent Technologies, Inc. Introduction Recent years have witnessed a rapid increase in the…
Key words
innovator, innovatorglycan, glycanglycans, glycansmab, mabsubunits, subunitsreleased, releasedcounts, countsassaymap, assaymapides, idesintact, intactagilent, agilentbravo, bravodeconvoluted, deconvolutedmass, massadvancebio
Application Note Pharma & Biopharma Critical Quality Attribute Monitoring of mAbs at the Intact and Subunit Levels Using a Cost-Effective, Simple and Robust LC/MS Solution Authors Linfeng Wu, Lisa Zang, and Guannan Li Agilent Technologies, Inc. Abstract Recombinant monoclonal antibodies…
Key words
mass, massnistmab, nistmabcounts, countsdeglycosylated, deglycosylatedintact, intactmsd, msdprotein, proteincharge, chargespectrum, spectrumdeconvoluted, deconvolutedglycoforms, glycoformsmabs, mabsresponse, responsechemstation, chemstationnone
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